Detection of monoclonal or oligoclonal integration of bovine leukemia virus proviral DNA by inverse polymerase chain reaction for diagnosis of enzootic bovine leucosis

2021 ◽  
Author(s):  
Masaki Maezawa ◽  
Kana Sakaguchi ◽  
Yusuke Tanaka ◽  
Ken-ichi Watanabe ◽  
Yoshiyasu Kobayashi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Inverse Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Proviral Dna ◽  
Polymerase Chain ◽  
Enzootic Bovine Leucosis

scholarly journals Detection of Bovine Leukemia Virus by in Situ Polymerase Chain Reaction in Tissues from a Heifer Diagnosed with Sporadic Thymic Lymphosarcoma

2005 ◽  
Vol 17 (2) ◽  
pp. 190-194 ◽  
Author(s):  
Robert B. Duncan ◽  
William K. Scarratt ◽  
Gertrude C. Buehring
Keyword(s):  
Polymerase Chain Reaction ◽  
Epithelial Cells ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Sudden Onset ◽  
Chain Reaction ◽  
Liver And Kidney ◽  
Cellular Tropism ◽  
Polymerase Chain

An 18-month-old bovine heifer was presented for clinical evaluation after a sudden onset of ventral edema. Clinical and pathological evaluations were consistent with thymic lymphosarcoma, a sporadic form of lymphosarcoma in cattle, which is not generally considered to be associated with bovine leukemia virus (BLV). This heifer was seropositive for BLV at 6 and 18 months of age. Tissues obtained at necropsy were evaluated using in situ polymerase chain reaction. The BLV proviral DNA was detected in lymphocytes of the thymus as well as in epithelial cells of the liver and kidney. This report presents evidence that thymic lymphosarcomas can be associated with BLV infection and that BLV may have a broader cellular tropism than was supposed previously.

scholarly journals Detection of Bovine Leukemia Virus in Blood and Milk by Nested and Real-Time Polymerase Chain Reactions

2003 ◽  
Vol 15 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Christopher J. Kuckleburg ◽  
Christopher C. Chase ◽  
Eric A. Nelson ◽  
Salvatore A. E. Marras ◽  
Matthew A. Dammen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Real Time ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain

Concerns about retroviruses in livestock and products derived from them have necessitated the development of tests to detect the bovine leukemia virus (BLV) in blood and milk from cattle. Dairy cattle ( n = 101) from 5 different geographical areas were used for this study. A nested polymerase chain reaction (PCR) identified 98% of BLV seropositive cattle ( n = 80) from blood and 65% from milk, whereas real-time PCR detected 94% of BLV seropositive cattle from blood and 59% from milk. Bovine leukemia virus was also detected by PCR in approximately 10% of seronegative cattle ( n = 21), most likely because of early detection before seroconversion.

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