A sandwich electrochemical immunosensor for Salmonella pullorum and Salmonella gallinarum based on a screen-printed carbon electrode modified with an ionic liquid and electrodeposited gold nanoparticles

2015 ◽  
Vol 182 (13-14) ◽  
pp. 2267-2275 ◽  
Author(s):  
Jianfeng Fei ◽  
Wenchao Dou ◽  
Guangying Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Ionic Liquid ◽  
Electrochemical Immunosensor ◽  
Carbon Electrode ◽  
Salmonella Gallinarum ◽  
Screen Printed Carbon Electrode ◽  
Salmonella Pullorum ◽  
Electrodeposited Gold Nanoparticles ◽  
Screen Printed

A sandwich electrochemical immunoassay for Salmonella pullorum and Salmonella gallinarum based on a AuNPs/SiO2/Fe3O4adsorbing antibody and 4 channel screen printed carbon electrode electrodeposited gold nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (91) ◽  
pp. 74548-74556 ◽  
Author(s):  
Jianfeng Fei ◽  
Wenchao Dou ◽  
Guangying Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Detection ◽  
Carbon Electrode ◽  
Electrochemical Immunoassay ◽  
Salmonella Gallinarum ◽  
Screen Printed Carbon Electrode ◽  
Salmonella Pullorum ◽  
Electrodeposited Gold Nanoparticles ◽  
Screen Printed

A: The synthesis of Fe3O4/SiO2–SH; B: AuNPs and the Ab1link with Fe3O4/SiO2–SH; C: functions of IMB for antigen enrichment and separation from the sample, and HRP-antibody for the immunoreaction; D: the principle of the electrochemical detection.

A microfluidic device based on a screen-printed carbon electrode with electrodeposited gold nanoparticles for the detection of IgG anti-Trypanosoma cruzi antibodies

The Analyst ◽  
2011 ◽  
Vol 136 (22) ◽  
pp. 4745 ◽  
Author(s):  
Sirley V. Pereira ◽  
Franco A. Bertolino ◽  
Martín A. Fernández-Baldo ◽  
Germán A. Messina ◽  
Eloy Salinas ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Trypanosoma Cruzi ◽  
Microfluidic Device ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Electrodeposited Gold Nanoparticles ◽  
Screen Printed

scholarly journals Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman
Keyword(s):  
Graphene Oxide ◽  
Limit Of Detection ◽  
Food Product ◽  
Electrochemical Immunosensor ◽  
Growth Promoter ◽  
Carbon Electrode ◽  
Electrochemical Assay ◽  
Sensor Platform ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode

2020 ◽  
Vol 833 ◽  
pp. 171-175
Author(s):  
Nurul Azurin Badruzaman ◽  
Mohd Azraie Mohd Azmi ◽  
Nur Azura Mohd Said
Keyword(s):  
Incubation Time ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Graphene Nanoplatelets ◽  
Carbon Electrode ◽  
Functionalized Graphene ◽  
Working Electrode ◽  
Screen Printed Carbon Electrode ◽  
Rabbit Igg ◽  
Screen Printed

We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphene-nanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers.

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