Determination of the activity of uracil-DNA glycosylase by using two-tailed reverse transcription PCR and gold nanoparticle-mediated silver nanocluster fluorescence: a new method for gene therapy-related enzyme detection

Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55.7% of the samples; viruses of the G10 and G8 serotypes were found in 34.9 and 4.7% of the samples, respectively. The virus in one sample (0.7%) was not classified due to concomitant infection with G6 and G8 strains, whereas viruses in six samples (4.0%) could not be characterized with any of the three G serotype-specific primers selected for the present study. When examined for their P-serotype specificities, viruses in 55 and 42.3% of the samples were characterized as P[11] and P[5], respectively, no P[1] serotype was identified, and viruses in 2.7% of the samples could not be classified due to multiple reactivity with both P[5]- and P[11]-specific primers. Various combinations of G and P serotypes were observed, the most frequent being G6,P[5] (38.3%), G10,P[11] (31.5%), and G6,P[11] (15.4%). The results of the present study, while contributing to a better understanding of the epidemiology of bovine rotaviruses in Italy, address the relevance of serotype specificity with regard to the constancy of the quality of bovine rotavirus vaccines under different field conditions.

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Application of quantitative reverse-transcription PCR (qRT-PCR) for the determination of the total viable count (TVC) on meat samples

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2009.04636.x ◽

2009 ◽

Author(s):

A. Dolan ◽

C.M. Burgess ◽

S. Fanning ◽

G. Duffy

Keyword(s):

Reverse Transcription ◽

Viable Count ◽

Total Viable Count ◽

Qrt Pcr ◽

Quantitative Reverse Transcription Pcr ◽

Reverse Transcription Pcr ◽

Meat Samples

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Determination of Human Rotavirus VP6 Genogroups I and II by Reverse Transcription-PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.00432-08 ◽

2008 ◽

Vol 46 (10) ◽

pp. 3330-3337 ◽

Cited By ~ 11

Author(s):

Y.-P. Lin ◽

C.-L. Kao ◽

S.-Y. Chang ◽

K. Taniguchi ◽

P.-Y. Hung ◽

...

Keyword(s):

Reverse Transcription ◽

Reverse Transcription Pcr ◽

Human Rotavirus ◽

Rotavirus Vp6

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Gastrodia elata powder capsule enhances anti-epileptic effect of carbamazepine by decreasing P-gp expression

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i9.11 ◽

2021 ◽

Vol 18 (9) ◽

pp. 1859-1865

Author(s):

Xiangji Dang ◽

Pei Zhao ◽

Yan Liu ◽

Long Qin ◽

Haisheng Jiao

Keyword(s):

Drug Resistance ◽

Western Blot ◽

Reverse Transcription ◽

Normal Saline ◽

Control Group ◽

Behavioral Characteristics ◽

Gastrodia Elata ◽

Reverse Transcription Pcr ◽

Protein Expressions

Purpose: To investigate the influence of Gastrodia elata powder capsule (GC) or gastrodin (GTD) on the anti-epileptic effect of carbamazepine (CBZ) on penicillin (PG)-induced epilepsy in rats. Methods: A total 116 rats were used in this study. Rats in the control group (n = 8) were injected with normal saline (NS) in place PG. Epilepsy was induced in the remaining 108 rats on the first day via PG injection. The rats were then divided randomly into six groups (18 rats per group): PG group, CBZ group, CBZ + GC group, CBZ + GTD group, GC group, and GTD group, which were given (p.o.) NS, CBZ (100 mg/kg), CBZ (100 mg/kg.) + GC (350 mg/kg), CBZ (100 mg/kg) + GTD (100 mg/kg), GC (350 mg/kg), and GTD (100 mg/kg), respectively, once a day for 15 days. The behavioral characteristics of the rats were observed and used to assess the anti-epileptic effect of the test drugs. Real-time quantitative reverse transcription-PCR and Western blot assays were employed for the determination of the effect of CBZ, GC and GTD on the expression levels of P-gp. Results: CBZ significantly reduced the symptoms of epilepsy, while GC and GTD enhanced the antiepileptic effect of CBZ, and reversed the CBZ-induced increases in the protein expressions of mrd1a and P-gp (p < 0.05). Conclusion: GC reverses CBZ drug resistance, probably through downregulation of P-gp expression. This finding indicates that GC is a potential anti-epilepsy drug, but it merits further studies.

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