The nuclear localization signal sequence of porcine circovirus type 2 ORF2 enhances intracellular delivery of plasmid DNA

2011 ◽  
Vol 156 (5) ◽  
pp. 803-815 ◽  
Author(s):  
Hsu-Chung Gabriel Chen ◽  
Siao-Ting Chiou ◽  
Jin-Yi Zheng ◽  
Shu-Hsiang Yang ◽  
Shiow-Suey Lai ◽  
...  
Keyword(s):  
Nuclear Localization ◽  
Plasmid Dna ◽  
Nuclear Localization Signal ◽  
Signal Sequence ◽  
Intracellular Delivery ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Nuclear Localization Signal Sequence ◽  
Localization Signal

scholarly journals Properties of expression of protein capside porcine circovirus type 2 in bacterial cells

2021 ◽  
pp. 48-57
Author(s):  
Anastasia D. Titova ◽  
Kirill V. Kudzin ◽  
Vladimir A. Prokulevich
Keyword(s):  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Bacterial Cells ◽  
Reading Frame ◽  
E Coli ◽  
Localization Signal

To improve expression of the porcine circovirus type 2 (PCV2) capsid protein in E. coli cells, the corresponding gene was optimized and two variants of the open reading frame were constructed, which encoded the full-sized and shortened capsid proteins as part of the expression vector. Rare codons were replaced, and in the case of a shortened version of the gene, the region corresponding to the N-terminal domain of the protein was deleted. A comparison was made of the expression level of the studied proteins. It was established that the highest level of expression in bacterial cells is achieved by simultaneously optimizing the codons and removing the initial (N-terminal) 108 base pair (bp) portion of the gene, which contains the nuclear localization signal.

scholarly journals Human Cytomegalovirus UL84 Protein Contains Two Nuclear Export Signals and Shuttles between the Nucleus and the Cytoplasm

2006 ◽  
Vol 80 (20) ◽  
pp. 10274-10280 ◽  
Author(s):  
Peter Lischka ◽  
Claudia Rauh ◽  
Regina Mueller ◽  
Thomas Stamminger
Keyword(s):  
Human Cytomegalovirus ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Nuclear Export ◽  
Signal Sequence ◽  
Regulatory Protein ◽  
Nucleocytoplasmic Shuttling ◽  
Viral Dna ◽  
Nuclear Localization Signal Sequence ◽  
Localization Signal

ABSTRACT Previous studies defined pUL84 of human cytomegalovirus as an essential regulatory protein with nuclear localization that was proposed to act during initiation of viral-DNA synthesis. Recently, we demonstrated that a complex domain of 282 amino acids within pUL84 functions as a nonconventional nuclear localization signal. Sequence inspection of this domain revealed the presence of motifs with homology to leucine-rich nuclear export signals. Here, we report the identification of two functional, autonomous nuclear export signals and show that pUL84 acts as a CRM-1-dependent nucleocytoplasmic shuttling protein. This suggests an unexpected cytoplasmic role for this essential viral regulatory protein.

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