Nuclear localization signal regulates porcine circovirus type 2 capsid protein nuclear export through phosphorylation

2018 ◽  
Vol 246 ◽  
pp. 12-22 ◽  
Author(s):  
Qiang Hou ◽  
Shaohua Hou ◽  
Qing Chen ◽  
Hong Jia ◽  
Ting Xin ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Nuclear Export ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Localization Signal

scholarly journals Properties of expression of protein capside porcine circovirus type 2 in bacterial cells

2021 ◽  
pp. 48-57
Author(s):  
Anastasia D. Titova ◽  
Kirill V. Kudzin ◽  
Vladimir A. Prokulevich
Keyword(s):  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Bacterial Cells ◽  
Reading Frame ◽  
E Coli ◽  
Localization Signal

To improve expression of the porcine circovirus type 2 (PCV2) capsid protein in E. coli cells, the corresponding gene was optimized and two variants of the open reading frame were constructed, which encoded the full-sized and shortened capsid proteins as part of the expression vector. Rare codons were replaced, and in the case of a shortened version of the gene, the region corresponding to the N-terminal domain of the protein was deleted. A comparison was made of the expression level of the studied proteins. It was established that the highest level of expression in bacterial cells is achieved by simultaneously optimizing the codons and removing the initial (N-terminal) 108 base pair (bp) portion of the gene, which contains the nuclear localization signal.

Comparing the efficiency of different Escherichia coli strains in producing recombinant capsid protein of porcine circovirus type 2

2020 ◽  
Vol 52 ◽  
pp. 101556 ◽  
Author(s):  
Vishal Rai ◽  
Vikramaditya Upmanyu ◽  
Gulam Mohd ◽  
Ravi Kumar ◽  
Sanganagouda Koppad ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Capsid Protein ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus
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