VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology

Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each,n=100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after precedingin vitromaturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P<0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.

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Fundamentals, Recent Advances, and Future Challenges in Bioimpedance Devices for Healthcare Applications

Journal of Sensors ◽

10.1155/2019/9210258 ◽

2019 ◽

Vol 2019 ◽

pp. 1-42 ◽

Cited By ~ 12

Author(s):

David Naranjo-Hernández ◽

Javier Reina-Tosina ◽

Mart Min

Keyword(s):

Healthcare Applications ◽

Body Composition Assessment ◽

Impedance Tomography ◽

Lab On Chip ◽

Recent Advances ◽

Chip Technology ◽

Impedance Pneumography ◽

Future Challenges ◽

On Chip ◽

Frequency Behavior

This work develops a thorough review of bioimpedance systems for healthcare applications. The basis and fundamentals of bioimpedance measurements are described covering issues ranging from the hardware diagrams to the configurations and designs of the electrodes and from the mathematical models that describe the frequency behavior of the bioimpedance to the sources of noise and artifacts. Bioimpedance applications such as body composition assessment, impedance cardiography (ICG), transthoracic impedance pneumography, electrical impedance tomography (EIT), and skin conductance are described and analyzed. A breakdown of recent advances and future challenges of bioimpedance is also performed, addressing topics such as transducers for biosensors and Lab-on-Chip technology, measurements in implantable systems, characterization of new parameters and substances, and novel bioimpedance applications.

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MICRO-SCALE TECHNOLOGIES FOR CELL MECHANICS: EXPERIMENTAL AND MODELLING APPROACHES

Istituto Lombardo - Accademia di Scienze e Lettere - Incontri di Studio ◽

10.4081/incontri.2018.388 ◽

2018 ◽

Author(s):

Federica Caselli ◽

Nicola A. Nodargi ◽

Paolo Bisegna

Keyword(s):

Cell Mechanics ◽

Cell Biology ◽

Single Cell Level ◽

Mechanical Function ◽

Label Free ◽

Cell Level ◽

Lab On Chip ◽

Non Invasive ◽

Chip Technology ◽

On Chip

Cell mechanics is a discipline that bridges cell biology with mechanics. Emerging microscale technologies are opening new venues in the field, due to their costeffectiveness, relatively easy fabrication, and high throughput. Two examples of those technologies are discussed here: microfluidic impedance cytometry and erythrocyte electrodeformation. The former is a lab-on-chip technology offering a simple, non-invasive, label-free method for counting, identifying and monitoring cellular biophysical and mechanical function at the single-cell level. The latter is a useful complement to the former, enabling cell deformation under the influence of an applied electric field.

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Lab-on-chip technology for chronic disease diagnosis

npj Digital Medicine ◽

10.1038/s41746-017-0014-0 ◽

2018 ◽

Vol 1 (1) ◽

Cited By ~ 33

Author(s):

Jiandong Wu ◽

Meili Dong ◽

Claudio Rigatto ◽

Yong Liu ◽

Francis Lin

Keyword(s):

Chronic Disease ◽

Disease Diagnosis ◽

Lab On Chip ◽

Chip Technology ◽

On Chip

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Molecular Detection of a Novel Human Influenza (H1N1) of Pandemic Potential by Conventional and Real-Time Quantitative RT-PCR Assays

Clinical Chemistry ◽

10.1373/clinchem.2009.130229 ◽

2009 ◽

Vol 55 (8) ◽

pp. 1555-1558 ◽

Cited By ~ 85

Author(s):

Leo L M Poon ◽

K H Chan ◽

G J Smith ◽

C S W Leung ◽

Y Guan ◽

...

Keyword(s):

Real Time ◽

Influenza A ◽

H1n1 Virus ◽

Influenza Viruses ◽

The Novel ◽

Human Influenza ◽

Rt Pcr ◽

Pcr Assay ◽

Recent Emergence ◽

Human H5n1

Abstract Background: Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed. Methods: We developed a conventional 1-step RT-PCR assay and a 1-step quantitative real-time RT-PCR assay to detect the novel H1N1 virus, but not the seasonal H1N1 viruses. We also developed an additional real-time RT-PCR that can discriminate the novel H1N1 from other swine and human H1 subtype viruses. Results: All of the assays had detection limits for the positive control in the range of 1.0 × 10−4 to 2.0 × 10−3 of the median tissue culture infective dose. Assay specificities were high, and for the conventional and real-time assays, all negative control samples were negative, including 7 human seasonal H1N1 viruses, 1 human H2N2 virus, 2 human seasonal H3N2 viruses, 1 human H5N1 virus, 7 avian influenza viruses (HA subtypes 4, 5, 7, 8, 9, and 10), and 48 nasopharyngeal aspirates (NPAs) from patients with noninfluenza respiratory diseases; for the assay that discriminates the novel H1N1 from other swine and human H1 subtype viruses, all negative controls were also negative, including 20 control NPAs, 2 seasonal human H1N1 viruses, 2 seasonal human H3N2 viruses, and 2 human H5N1 viruses. Conclusions: These assays appear useful for the rapid diagnosis of cases with the novel H1N1 virus, thereby allowing better pandemic preparedness.

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Thermal assisted direct bonding between structured glasses for lab-on-chip technology

Microsystem Technologies ◽

10.1007/s00542-009-0911-5 ◽

2009 ◽

Vol 15 (12) ◽

pp. 1873-1877 ◽

Cited By ~ 9

Author(s):

Qiuping Chen ◽

Qiuling Chen ◽

Daniel Milanese ◽

Monica Ferraris

Keyword(s):

Lab On Chip ◽

Chip Technology ◽

On Chip

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Industry Partnership: Lab on Chip Chemical Sensor Technology for Ocean Observing

Frontiers in Marine Science ◽

10.3389/fmars.2021.697611 ◽

2021 ◽

Vol 8 ◽

Author(s):

Matt Mowlem ◽

Alexander Beaton ◽

Robin Pascal ◽

Allison Schaap ◽

Socratis Loucaides ◽

...

Keyword(s):

Chemical Sensor ◽

Autonomous Underwater Vehicles ◽

Product Line ◽

The Arctic ◽

Sensor Technology ◽

Lab On Chip ◽

Chip Technology ◽

Wide Range ◽

On Chip

We introduce for the first time a new product line able to make high accuracy measurements of a number of water chemistry parameters in situ: i.e., submerged in the environment including in the deep sea (to 6,000 m). This product is based on the developments of in situ lab on chip technology at the National Oceanography Centre (NOC), and the University of Southampton and is produced under license by Clearwater Sensors Ltd., a start-up and industrial partner in bringing this technology to global availability and further developing its potential. The technology has already been deployed by the NOC, and with their partners worldwide over 200 times including to depths of ∼4,800 m, in turbid estuaries and rivers, and for up to a year in seasonally ice-covered regions of the arctic. The technology is capable of making accurate determinations of chemical and biological parameters that require reagents and which produce an electrical, absorbance, fluorescence, or luminescence signal. As such it is suitable for a wide range of environmental measurements. Whilst further parameters are in development across this partnership, Nitrate, Nitrite, Phosphate, Silicate, Iron, and pH sensors are currently available commercially. Theses sensors use microfluidics and optics combined in an optofluidic chip with electromechanical valves and pumps mounted upon it to mix water samples with reagents and measure the optical response. An overview of the sensors and the underlying components and technologies is given together with examples of deployments and integrations with observing platforms such as gliders, autonomous underwater vehicles and moorings.

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