scholarly journals MICRO-SCALE TECHNOLOGIES FOR CELL MECHANICS: EXPERIMENTAL AND MODELLING APPROACHES

2018 ◽  
Author(s):  
Federica Caselli ◽  
Nicola A. Nodargi ◽  
Paolo Bisegna
Keyword(s):  
Cell Mechanics ◽  
Cell Biology ◽  
Single Cell Level ◽  
Mechanical Function ◽  
Label Free ◽  
Cell Level ◽  
Lab On Chip ◽  
Non Invasive ◽  
Chip Technology ◽  
On Chip

Cell mechanics is a discipline that bridges cell biology with mechanics. Emerging microscale technologies are opening new venues in the field, due to their costeffectiveness, relatively easy fabrication, and high throughput. Two examples of those technologies are discussed here: microfluidic impedance cytometry and erythrocyte electrodeformation. The former is a lab-on-chip technology offering a simple, non-invasive, label-free method for counting, identifying and monitoring cellular biophysical and mechanical function at the single-cell level. The latter is a useful complement to the former, enabling cell deformation under the influence of an applied electric field.

scholarly journals Methods and applications of label-free cell-based systems

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Joachim Wiest
Keyword(s):  
Environmental Monitoring ◽  
Morphological Changes ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Controlled System ◽  
Chip Technology ◽  
Cell Monitoring ◽  
A Cell ◽  
On Chip

Label-free monitoring of living cells is used in various applications such as drug development, toxicology, regenerative medicine or environmental monitoring. The most prominent methods for monitoring the extracellular acidification, oxygen consumption, electrophysiological activity and morphological changes of living cells are described. Furthermore, the intelligent mobile lab (IMOLA) – a computer controlled system integrating cell monitoring and automated cell cultivation – is described as an example of a cell-based system for microphysiometry. Results from experiments in the field of environmental monitoring using algae are presented. An outlook toward the development of an organ-on-chip technology is given.

Label-Free Cancer Stem-like Cell Assay Conducted at a Single Cell Level Using Microfluidic Mechanotyping Devices

2021 ◽  
Author(s):  
Miyu Terada ◽  
Sachiko Ide ◽  
Toyohiro Naito ◽  
Niko Kimura ◽  
Michiya Matsusaki ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Cell Assay

A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4738 ◽  
Author(s):  
Mohamed Lemine Youba Diakité ◽  
Jerôme Champ ◽  
Stephanie Descroix ◽  
Laurent Malaquin ◽  
François Amblard ◽  
...  
Keyword(s):  
Long Range ◽  
Detection Method ◽  
Dna Detection ◽  
Low Cost ◽  
Label Free ◽  
Lab On Chip ◽  
Free Dna ◽  
Chip Format ◽  
Long Range Pcr ◽  
On Chip

scholarly journals High-resolution label-free 3D mapping of extracellular pH of single living cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanjun Zhang ◽  
Yasufumi Takahashi ◽  
Sung Pil Hong ◽  
Fengjie Liu ◽  
Joanna Bednarska ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Extracellular Ph ◽  
Single Cell Level ◽  
Ph Sensing ◽  
Label Free ◽  
Cell Level ◽  
Spatiotemporal Resolution ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

AbstractDynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.

scholarly journals Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Bartosz Kempisty ◽  
Rafał Walczak ◽  
Paweł Antosik ◽  
Patrycja Sniadek ◽  
Marta Rybska ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Spectral Characteristics ◽  
Oocyte Quality ◽  
Lab On Chip ◽  
Chip Technology ◽  
Porcine Oocyte ◽  
Follicular Size ◽  
On Chip ◽  
Pig Oocyte

Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each,n=100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after precedingin vitromaturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P<0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.

scholarly journals Fundamentals, Recent Advances, and Future Challenges in Bioimpedance Devices for Healthcare Applications

2019 ◽  
Vol 2019 ◽  
pp. 1-42 ◽  
Author(s):  
David Naranjo-Hernández ◽  
Javier Reina-Tosina ◽  
Mart Min
Keyword(s):  
Healthcare Applications ◽  
Body Composition Assessment ◽  
Impedance Tomography ◽  
Lab On Chip ◽  
Recent Advances ◽  
Chip Technology ◽  
Impedance Pneumography ◽  
Future Challenges ◽  
On Chip ◽  
Frequency Behavior

This work develops a thorough review of bioimpedance systems for healthcare applications. The basis and fundamentals of bioimpedance measurements are described covering issues ranging from the hardware diagrams to the configurations and designs of the electrodes and from the mathematical models that describe the frequency behavior of the bioimpedance to the sources of noise and artifacts. Bioimpedance applications such as body composition assessment, impedance cardiography (ICG), transthoracic impedance pneumography, electrical impedance tomography (EIT), and skin conductance are described and analyzed. A breakdown of recent advances and future challenges of bioimpedance is also performed, addressing topics such as transducers for biosensors and Lab-on-Chip technology, measurements in implantable systems, characterization of new parameters and substances, and novel bioimpedance applications.

Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat

2019 ◽  
Vol 2 (12) ◽  
pp. 5880-5887 ◽  
Author(s):  
Francesca Costantini ◽  
Nicola Lovecchio ◽  
Albert Ruggi ◽  
Cesare Manetti ◽  
Augusto Nascetti ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescent Label ◽  
Label Free ◽  
Lab On Chip ◽  
On Chip

Alterations of Raman profiles of nasopharyngeal carcinoma cells after treated with chemodrugs detected by laser tweezer Raman spectroscopy.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15513-e15513
Author(s):  
Miaomiao Li ◽  
Xiaochuan Chen ◽  
Ting Lin ◽  
Zongwei Huang ◽  
Shihong Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Single Cell ◽  
Control Group ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Analytical Strategy ◽  
Linear Discriminant ◽  
Laser Tweezer

e15513 Background: To explore the metabolic alterations of nasopharyngeal carcinoma (NPC) cells after treated with chemodrugs, the Raman profiles were characterized with laser tweezer Raman spectroscopy. Methods: Two NPC cell lines (CNE2 and C666-1) were treated with gemcitabine, cisplatin, and paclitaxel, respectively. The high-quality Raman spectra of cells without or with treatments were recorded at the single-cell level with label-free laser tweezers Raman spectroscopy (LTRS) and analyzed for the differences of alterations of Raman profiles. Results: Tentative assignments of Raman peaks indicated that the cellular specific biomolecular changes associated with drug treatment, including changes in protein structure (e.g. 1655 cm−1), changes in DNA content and structure (e.g. 830 cm−1), destruction of DNA base pairs (e.g. 785 cm−1), and reduction in lipids (e.g. 970 cm−1). Besides, both principal components analysis (PCA) combined with linear discriminant analysis (LDA) and the classification and regression trees (CRT) algorithms were employed to further analyze and classify the spectral data between control group and treated group, with the best discriminant accuracy of 96.7% and 90.0% for CNE2 and C666-1 group treated with paclitaxel, respectively. Conclusions: This exploratory work demonstrated that LTRS technology combined with multivariate statistical analysis has promising potential to be a novel analytical strategy at the single-cell level for the evaluation of NPC-related chemotherapeutic drugs.

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