Thermal assisted direct bonding between structured glasses for lab-on-chip technology

2009 ◽  
Vol 15 (12) ◽  
pp. 1873-1877 ◽  
Author(s):  
Qiuping Chen ◽  
Qiuling Chen ◽  
Daniel Milanese ◽  
Monica Ferraris
Keyword(s):  
Lab On Chip ◽  
Chip Technology ◽  
On Chip

scholarly journals Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Bartosz Kempisty ◽  
Rafał Walczak ◽  
Paweł Antosik ◽  
Patrycja Sniadek ◽  
Marta Rybska ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Spectral Characteristics ◽  
Oocyte Quality ◽  
Lab On Chip ◽  
Chip Technology ◽  
Porcine Oocyte ◽  
Follicular Size ◽  
On Chip ◽  
Pig Oocyte

Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each,n=100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after precedingin vitromaturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P<0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.

scholarly journals Fundamentals, Recent Advances, and Future Challenges in Bioimpedance Devices for Healthcare Applications

2019 ◽  
Vol 2019 ◽  
pp. 1-42 ◽  
Author(s):  
David Naranjo-Hernández ◽  
Javier Reina-Tosina ◽  
Mart Min
Keyword(s):  
Healthcare Applications ◽  
Body Composition Assessment ◽  
Impedance Tomography ◽  
Lab On Chip ◽  
Recent Advances ◽  
Chip Technology ◽  
Impedance Pneumography ◽  
Future Challenges ◽  
On Chip ◽  
Frequency Behavior

This work develops a thorough review of bioimpedance systems for healthcare applications. The basis and fundamentals of bioimpedance measurements are described covering issues ranging from the hardware diagrams to the configurations and designs of the electrodes and from the mathematical models that describe the frequency behavior of the bioimpedance to the sources of noise and artifacts. Bioimpedance applications such as body composition assessment, impedance cardiography (ICG), transthoracic impedance pneumography, electrical impedance tomography (EIT), and skin conductance are described and analyzed. A breakdown of recent advances and future challenges of bioimpedance is also performed, addressing topics such as transducers for biosensors and Lab-on-Chip technology, measurements in implantable systems, characterization of new parameters and substances, and novel bioimpedance applications.

scholarly journals MICRO-SCALE TECHNOLOGIES FOR CELL MECHANICS: EXPERIMENTAL AND MODELLING APPROACHES

2018 ◽  
Author(s):  
Federica Caselli ◽  
Nicola A. Nodargi ◽  
Paolo Bisegna
Keyword(s):  
Cell Mechanics ◽  
Cell Biology ◽  
Single Cell Level ◽  
Mechanical Function ◽  
Label Free ◽  
Cell Level ◽  
Lab On Chip ◽  
Non Invasive ◽  
Chip Technology ◽  
On Chip

Cell mechanics is a discipline that bridges cell biology with mechanics. Emerging microscale technologies are opening new venues in the field, due to their costeffectiveness, relatively easy fabrication, and high throughput. Two examples of those technologies are discussed here: microfluidic impedance cytometry and erythrocyte electrodeformation. The former is a lab-on-chip technology offering a simple, non-invasive, label-free method for counting, identifying and monitoring cellular biophysical and mechanical function at the single-cell level. The latter is a useful complement to the former, enabling cell deformation under the influence of an applied electric field.

scholarly journals Lab-on-chip technology for chronic disease diagnosis

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Jiandong Wu ◽  
Meili Dong ◽  
Claudio Rigatto ◽  
Yong Liu ◽  
Francis Lin
Keyword(s):  
Chronic Disease ◽  
Disease Diagnosis ◽  
Lab On Chip ◽  
Chip Technology ◽  
On Chip

scholarly journals Industry Partnership: Lab on Chip Chemical Sensor Technology for Ocean Observing

2021 ◽  
Vol 8 ◽  
Author(s):  
Matt Mowlem ◽  
Alexander Beaton ◽  
Robin Pascal ◽  
Allison Schaap ◽  
Socratis Loucaides ◽  
...  
Keyword(s):  
Chemical Sensor ◽  
Autonomous Underwater Vehicles ◽  
Product Line ◽  
The Arctic ◽  
Sensor Technology ◽  
Lab On Chip ◽  
Chip Technology ◽  
Wide Range ◽  
On Chip

We introduce for the first time a new product line able to make high accuracy measurements of a number of water chemistry parameters in situ: i.e., submerged in the environment including in the deep sea (to 6,000 m). This product is based on the developments of in situ lab on chip technology at the National Oceanography Centre (NOC), and the University of Southampton and is produced under license by Clearwater Sensors Ltd., a start-up and industrial partner in bringing this technology to global availability and further developing its potential. The technology has already been deployed by the NOC, and with their partners worldwide over 200 times including to depths of ∼4,800 m, in turbid estuaries and rivers, and for up to a year in seasonally ice-covered regions of the arctic. The technology is capable of making accurate determinations of chemical and biological parameters that require reagents and which produce an electrical, absorbance, fluorescence, or luminescence signal. As such it is suitable for a wide range of environmental measurements. Whilst further parameters are in development across this partnership, Nitrate, Nitrite, Phosphate, Silicate, Iron, and pH sensors are currently available commercially. Theses sensors use microfluidics and optics combined in an optofluidic chip with electromechanical valves and pumps mounted upon it to mix water samples with reagents and measure the optical response. An overview of the sensors and the underlying components and technologies is given together with examples of deployments and integrations with observing platforms such as gliders, autonomous underwater vehicles and moorings.

Optically Induced Rapid Electrokinetic Patterning of Non-Spherical Particles: Study of Colloidal Phase Transition

2010 ◽  
Author(s):  
Raviraj Vijay Thakur ◽  
Steven Wereley
Keyword(s):  
Phase Transition ◽  
Fluid Mechanics ◽  
Electrode Surface ◽  
Colloidal Particles ◽  
Spherical Particles ◽  
Lab On Chip ◽  
Chip Technology ◽  
Sorting Technique ◽  
On Chip ◽  
Rapid Electrokinetic Patterning

Patterning of colloidal particles on surfaces is an application that has evinced wide interest from the fluid mechanics community, due to its possible applicability in a number of engineering situations such as manufacture of photonic crystals[1], bioengineering tissues[2] and lab on chip technology[3], etc. Recently Kumar et al. had proposed the technique of rapid electrokinetic patterning (REP) [4], a hybrid opto-electric manipulation technique that can manipulate and pattern colloidal particles on an electrode surface. REP utilizes optical landscapes to create local gradients in temperature on an electrode substrate. This allows local changes in permittivity and conductivity of the fluid. Colloidal particles can then be dynamically patterned at the illuminated locations of the electrode surface. REP can be used for capturing selective group of particles and thus it serves as a sorting technique too [5].

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

2020 ◽  
Vol 46 (05) ◽  
pp. 622-636
Author(s):  
Crispin Szydzik ◽  
Rose J. Brazilek ◽  
Warwick S. Nesbitt
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Diagnostic Tools ◽  
Microfluidic Systems ◽  
Experimental Conditions ◽  
Lab On Chip ◽  
Design Considerations ◽  
Chip Technology ◽  
On Chip

AbstractThe manipulation of blood within in vitro environments presents a persistent challenge, due to the highly reactive nature of blood, and its multifaceted response to material contact, changes in environmental conditions, and stimulation during handling. Microfluidic Lab-on-Chip systems offer the promise of robust point-of-care diagnostic tools and sophisticated research platforms. The capacity for precise control of environmental and experimental conditions afforded by microfluidic technologies presents unique opportunities that are particularly relevant to research and clinical applications requiring the controlled manipulation of blood. A critical bottleneck impeding the translation of existing Lab-on-Chip technology from laboratory bench to the clinic is the ability to reliably handle relatively small blood samples without negatively impacting blood composition or function. This review explores design considerations critical to the development of microfluidic systems intended for use with whole blood from an engineering perspective. Material hemocompatibility is briefly explored, encompassing common microfluidic device materials, as well as surface modification strategies intended to improve hemocompatibility. Operational hemocompatibility, including shear-induced effects, temperature dependence, and gas interactions are explored, microfluidic sample preparation methodologies are introduced, as well as current techniques for on-chip manipulation of the whole blood. Finally, methods of assessing hemocompatibility are briefly introduced, with an emphasis on primary hemostasis and platelet function.

scholarly journals Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms

2017 ◽  
Vol 8 ◽  
pp. 204173141774150 ◽  
Author(s):  
Lidia Escutia-Guadarrama ◽  
Genaro Vázquez-Victorio ◽  
David Martínez-Pastor ◽  
Brenda Nieto-Rivera ◽  
Marcela Sosa-Garrocho ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Type I Collagen ◽  
Microcontact Printing ◽  
Low Cost ◽  
Extracellular Matrix Protein ◽  
Type I ◽  
Human Hepatoma Cells ◽  
Lab On Chip ◽  
Chip Technology ◽  
On Chip

We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications. A low-cost CD-DVD laser was used to etch biomimetic micropatterns into acrylic sheets that were in turn replicated to poly-dimethylsiloxane slabs with the desired features. These stamps were finally inked with type I collagen for microcontact printing transfer on the culture substrates in a simple manner. Human hepatoma cells (HepG2) and rat primary hepatocytes, which do not adhere to bare poly-dimethylsiloxane, were successfully seeded and showed optimal adhesion and survival on simple protein micropatterns with a hepatic cord geometry in order to validate our technique. HepG2 cells also proliferated on the stamps. Soft and stiff poly-dimethylsiloxane layers were also tested to demonstrate that our cost-effective process is compatible with biomimetic organ-on-chip technology integrating tunable stiffness with a potential application to drug testing probes development where such cells are commonly used.

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