Molecular identification of a new begomovirus associated with yellow mosaic disease of Jatropha gossypifolia in India

2011 ◽  
Vol 156 (12) ◽  
pp. 2303-2307 ◽  
Author(s):  
S. K. Snehi ◽  
S. K. Raj ◽  
M. S. Khan ◽  
V. Prasad
Keyword(s):  
Molecular Identification ◽  
Mosaic Disease ◽  
Yellow Mosaic Disease ◽  
Jatropha Gossypifolia

scholarly journals Molecular Characterization of Mungbean Yellow Mosaic Disease and Coat Protein Gene in Mungbean Varieties of Bangladesh

2012 ◽  
Vol 22 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Mohammad Nurul Islam ◽  
Sonia Khan Sony ◽  
Rita Sarah Borna
Keyword(s):  
Coat Protein ◽  
Molecular Identification ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Yellow Mosaic Disease ◽  
Resistant Varieties ◽  
Sequence Similarities

Mungben yellow mosaic virus (MYMV), a member of the genus begomovirus causing major yield loss of mungbean. Development of MYMV tolerant/resistant varieties using an antiviral strategy may be one of the potential options to overcome MYMV diseases. In the present investigation, molecular identification of yellow mosaic disease and characterization of (MYMV) coat protein (CP) gene was attempted. Yellow mosaic symptomatic samples were identified through PCR using conserved region primers designed after alignment of the available CP sequences in NCBI data base. Sequence analysis of the PCR amplified samples showed 97% sequence similarities with the coat protein gene of Mungbean Yellow Mosaic India Virus (MYMIV) Bangladesh strain and was designated as MYMIV-BD. This appears to be the first report of the molecular identification of MYMV in Bangladesh. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11263Plant Tissue Cult. & Biotech. 22(1): 73-81, 2012 (June)

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

Survey for Yellow Mosaic Disease Occurring on Blackgram [Vigna mungo (L.) Hepper] and Weed Hosts in Four Major Blackgram Growing Districts of Andhra Pradesh and Characterization of Associated Viruses using Coat Protein Gene

2021 ◽  
Author(s):  
Vallabhaneni Tilak Chowdary ◽  
V. Manoj Kumar ◽  
P. Kishore Varma ◽  
B. Sreekanth ◽  
V. Srinivasa Rao
Keyword(s):  
Coat Protein ◽  
Andhra Pradesh ◽  
Disease Incidence ◽  
Pcr Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Weed Plants ◽  
Weed Hosts

Background: Yellow mosaic disease (YMD) caused by Yellow mosaic virus is one of the major constraints in the pulse production in Andhra Pradesh (A.P.) due to fast evolution of strains, like Mungbean yellow mosaic India virus (MYMIV). Keeping this in view, a survey was undertaken in the major blackgram growing districts of A.P. to know the YMD incidence in blackgram and weed hosts and were characterized based on genetic features by comparing with other YMV isolates from different hosts and locations across the world. Methods: Roving survey was conducted during rabi 2019-20 in major blackgram growing districts of A.P. viz., Krishna, Guntur, West Godavari and Prakasam districts for YMD incidence. Blackgram plants showing characteristic symptoms were collected as representative samples from each mandal along with the suspected weed plants and were subjected to amplification using coat protein (CP) specific primers followed by molecular characterization. Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny. Result: Out of the four districts surveyed, the highest disease incidence was recorded at Machavaram village of Prakasam district (43.22%), whereas least disease incidence was recorded at Chinaganjam village of Praksam district (2.4%). Six weeds viz., Ageratum conyzoides, Amaranthus viridis, Parthenium hysterophporus, Vigna trilobata, Abelmoscus moschatus, Desmodium laxiflorum have showed positive result in PCR amplification with MYMIV specific coat protein primers. Four isolates from blackgram samples and two from weed plants shared 94.85 to 99.58% nucleotide identity among themselves.

Quantification of a legume begomovirus to evaluate soybean genotypes for resistance to yellow mosaic disease

2019 ◽  
Vol 268 ◽  
pp. 24-31 ◽  
Author(s):  
Shunmugiah V. Ramesh ◽  
Maranna Shivakumar ◽  
Rajkumar Ramteke ◽  
Virender S. Bhatia ◽  
Bhagat S. Chouhan ◽  
...  
Keyword(s):  
Mosaic Disease ◽  
Yellow Mosaic Disease ◽  
Soybean Genotypes

scholarly journals High-resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease

2020 ◽  
Author(s):  
Hélène Pidon ◽  
Neele Wendler ◽  
Antje Habekuβ ◽  
Anja Maasberg ◽  
Brigitte Ruge-Wehling ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Mechanism ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Linkage Drag ◽  
Resistance Locus ◽  
Wild Relative ◽  
Yellow Mosaic Disease ◽  
Barley Breeding

Abstract Key message We mapped the Rym14Hb resistance locus to barley yellow mosaic disease in a 2Mbp interval. The co-segregating markers will be instrumental for marker-assisted selection in barley breeding. Abstract Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14Hb is a dominant major resistance gene on chromosome 6HS, originating from barley’s secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n = 7500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.

Mungbean yellow mosaic India virusis associated with yellow mosaic disease of blackgram (Vigna mungoL.) in Andhra Pradesh, India

2014 ◽  
Vol 48 (4) ◽  
pp. 345-353 ◽  
Author(s):  
B.V. Bhaskara Reddy ◽  
S. Obaiah ◽  
L. Prasanthi ◽  
Y. Sivaprasad ◽  
A. Sujitha ◽  
...  
Keyword(s):  
Andhra Pradesh ◽  
Mosaic Disease ◽  
Yellow Mosaic Disease

scholarly journals Molecular evidence for existence of a New World begomovirus associated with yellow mosaic disease ofCorchorus capsularisin India

2008 ◽  
Vol 3 (1) ◽  
pp. 59 ◽  
Author(s):  
R. Ghosh ◽  
S. Paul ◽  
S. Das ◽  
P. Palit ◽  
S. Acharyya ◽  
...  
Keyword(s):  
New World ◽  
Mosaic Disease ◽  
Molecular Evidence ◽  
Yellow Mosaic Disease
Sign in / Sign up

Export Citation Format

Share Document