Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms

2012 ◽  
Vol 157 (7) ◽  
pp. 1323-1328 ◽  
Author(s):  
Liang Jiang ◽  
Genhong Wang ◽  
Tingcai Cheng ◽  
Qiong Yang ◽  
Shengkai Jin ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Transgenic Silkworms ◽  
Antiviral Gene

scholarly journals Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus

2017 ◽  
Vol 91 (8) ◽  
Author(s):  
Shuqing Chen ◽  
Chengxiang Hou ◽  
Honglun Bi ◽  
Yueqiang Wang ◽  
Jun Xu ◽  
...  
Keyword(s):  
Antiviral Therapy ◽  
Bombyx Mori ◽  
Transgenic Animals ◽  
Viral Gene ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Guide Rnas ◽  
Virus Clearance ◽  
Short Palindromic Repeat ◽  
Transgenic Silkworms ◽  
Pathogen Genome

ABSTRACT We developed a novel antiviral strategy by combining transposon-based transgenesis and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system for the direct cleavage of Bombyx mori nucleopolyhedrovirus (BmNPV) genome DNA to promote virus clearance in silkworms. We demonstrate that transgenic silkworms constitutively expressing Cas9 and guide RNAs targeting the BmNPV immediate early-1 (ie-1) and me53 genes effectively induce target-specific cleavage and subsequent mutagenesis, especially large (∼7-kbp) segment deletions in BmNPV genomes, and thus exhibit robust suppression of BmNPV proliferation. Transgenic animals exhibited higher and inheritable resistance to BmNPV infection than wild-type animals. Our approach will not only contribute to modern sericulture but also shed light on future antiviral therapy. IMPORTANCE Pathogen genome targeting has shown its potential in antiviral research. However, transgenic CRISPR/Cas9 system-mediated viral genome targeting has not been reported as an antiviral strategy in a natural animal host of a virus. Our data provide an effective approach against BmNPV infection in a real-world biological system and demonstrate the potential of transgenic CRISPR/Cas9 systems in antiviral research in other species.

scholarly journals Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

2012 ◽  
Vol 86 (13) ◽  
pp. 7345-7359 ◽  
Author(s):  
J. Xue ◽  
N. Qiao ◽  
W. Zhang ◽  
R.-L. Cheng ◽  
X.-Q. Zhang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Transcriptome Analysis ◽  
Host Cells ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Dynamic Interactions

Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus

2012 ◽  
Vol 40 (2) ◽  
pp. 865-873
Author(s):  
Yong Liu ◽  
Feng Yu ◽  
Huiling Wu ◽  
Qing Cao ◽  
Yu Wu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Open Reading Frame ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Reading Frame

scholarly journals Bombyx mori nucleopolyhedrovirus protein Bm11 is involved in occlusion body production and occlusion-derived virus embedding

Virology ◽  
2019 ◽  
Vol 527 ◽  
pp. 12-20 ◽  
Author(s):  
Haiping Wang ◽  
Weifan Xu ◽  
Xiangshuo Kong ◽  
Ying Fan ◽  
Xiaofeng Wu
Keyword(s):  
Bombyx Mori ◽  
Occlusion Body ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Body Production

A constitutive super-enhancer: homologous region 3 of Bombyx mori nucleopolyhedrovirus

2004 ◽  
Vol 318 (4) ◽  
pp. 1039-1044 ◽  
Author(s):  
Yin Chen ◽  
Bin Yao ◽  
Zhongze Zhu ◽  
Yongzhu Yi ◽  
Xu’ai Lin ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Homologous Region ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Super Enhancer

Display of Bombyx mori Nucleopolyhedrovirus GP64 on the Bacillus subtilis Spore Coat

2011 ◽  
Vol 62 (5) ◽  
pp. 1368-1373 ◽  
Author(s):  
Guohui Li ◽  
Qi Tang ◽  
Huiqing Chen ◽  
Qin Yao ◽  
Degang Ning ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bombyx Mori ◽  
Spore Coat ◽  
Subtilis Spore ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Bacillus Subtilis Spore
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