Characterization of a soybean mosaic virus variant causing different diseases in Glycine max and Nicotiana benthamiana

2016 ◽  
Vol 162 (2) ◽  
pp. 549-553 ◽  
Author(s):  
Hua Jiang ◽  
Kai Li ◽  
Daolong Dou ◽  
Junyi Gai
Keyword(s):  
Glycine Max ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Soybean Mosaic Virus ◽  
Virus Variant

Characterization of broad-spectrum resistance to Soybean mosaic virus in soybean [Glycine max (L.) Merr.] cultivar ‘RN-9’

2018 ◽  
Vol 137 (4) ◽  
pp. 605-613 ◽  
Author(s):  
Rui Ren ◽  
Jinlong Yin ◽  
Huanfang Zheng ◽  
Tao Wang ◽  
Shichao Liu ◽  
...  
Keyword(s):  
Glycine Max ◽  
Mosaic Virus ◽  
Broad Spectrum ◽  
Soybean Mosaic Virus ◽  
Broad Spectrum Resistance ◽  
Glycine Max L

scholarly journals Genome-Wide Identification and Characterization of Long Noncoding RNAs Involved in Chinese Wheat Mosaic Virus Infection of Nicotiana benthamiana

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 232
Author(s):  
Weiran Zheng ◽  
Haichao Hu ◽  
Qisen Lu ◽  
Peng Jin ◽  
Linna Cai ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Genome Wide ◽  
Chinese Wheat

Recent studies have shown that a large number of long noncoding RNAs (lncRNAs) can regulate various biological processes in animals and plants. Although lncRNAs have been identified in many plants, they have not been reported in the model plant Nicotiana benthamiana. Particularly, the role of lncRNAs in plant virus infection remains unknown. In this study, we identified lncRNAs in N. benthamiana response to Chinese wheat mosaic virus (CWMV) infection by RNA sequencing. A total of 1175 lncRNAs, including 65 differentially expressed lncRNAs, were identified during CWMV infection. We then analyzed the functions of some of these differentially expressed lncRNAs. Interestingly, one differentially expressed lncRNA, XLOC_006393, was found to participate in CWMV infection as a precursor to microRNAs in N. benthamiana. These results suggest that lncRNAs play an important role in the regulatory network of N. benthamiana in response to CWMV infection.

Genetic analysis and fine-mapping of Soybean mosaic virus SC7 and SC13 resistance genes in soybean (Glycine max)

2020 ◽  
Vol 71 (5) ◽  
pp. 477
Author(s):  
Hexiang Luan ◽  
Yongkun Zhong ◽  
Dagang Wang ◽  
Rui Ren ◽  
Le Gao ◽  
...  
Keyword(s):  
Glycine Max ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Fine Mapping ◽  
Soybean Mosaic Virus ◽  
Resistance Breeding ◽  
Physical Distance ◽  
Breeding Systems ◽  
Chromosome 2 ◽  
Transcription Factor Genes

Soybean mosaic virus (SMV) is one of the most destructive pathogens of soybean (Glycine max (L.) Merr.) worldwide. In this study, 184 F7:11 recombinant inbred line (RIL) populations derived from Kefeng No. 1 × Nannong 1138-2 were used to study the inheritance and linkage mapping of resistance genes against SMV strains SC7 and SC13 in Kefeng No. 1. Two independent dominant genes (designated Rsc7 and Rsc13) that control resistance to SC7 and SC13 were located on a molecular linkage group (MLG) of chromosome 2 (D1b). A mixed segregating population was developed by self-pollination of three heterozygous plants of residual heterozygous lines (RHL3-27, RHL3-30, RHL3-53) with five markers linked to the loci, and was used in fine-mapping of Rsc7 and Rsc13. In addition, Rsc7 was fine-mapped between BARCSOYSSR_02_0667 and BARCSOYSSR_02_0670 on MLG D1b. The genetic distance between the two closest markers was 0.7 cM and the physical distance of the interval was ~77 kb, which included one LRR gene and another gene containing an F-box region. Two SSR markers (BARCSOYSSR_02_0610 and BARCSOYSSR_02_0621) were closely linked to the SC13 resistance gene. The physical distance where Rsc13 was located was ~191 kb. Sequence analysis showed that there were two K-box region types of transcription factor genes; GmHSP40 and two serine/threonine protein kinase (STK) genes were the most likely candidate genes. These results will facilitate map-based cloning of the Rsc7 and Rsc13 genes and development of transgenic disease-resistant varieties, and will provide SMV-resistance breeding systems with excellent resistance germplasm.

Characterization of Synergy between Cucumber mosaic virus and Tobacco necrosis virus in Nicotiana benthamiana

2007 ◽  
Vol 155 (9) ◽  
pp. 570-573 ◽  
Author(s):  
D. Xi ◽  
H. Feng ◽  
L. Lan ◽  
J. Du ◽  
J. Wang ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

scholarly journals Optimizing RNAi-Target by Nicotiana benthamiana-Soybean Mosaic Virus System Drives Broad Resistance to Soybean Mosaic Virus in Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Hua Jiang ◽  
Kai Li ◽  
Junyi Gai
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Soybean Mosaic Virus ◽  
Multiple Resistance ◽  
Genome Sequences ◽  
Multiple Sequence ◽  
Detached Leaf ◽  
Broad Spectrum Resistance ◽  
Inoculation Assay ◽  
Virus System

Soybean mosaic virus (SMV) is a prevalent pathogen of soybean (Glycine max). Pyramiding multiple SMV-resistance genes into one individual is tedious and difficult, and even if successful, the obtained multiple resistance might be broken by pathogen mutation, while targeting viral genome via host-induced gene silencing (HIGS) has potential to explore broad-spectrum resistance (BSR) to SMV. We identified five conserved target fragments (CTFs) from S1 to S5 using multiple sequence alignment of 30 SMV genome sequences and assembled the corresponding target-inverted-repeat constructs (TIRs) from S1-TIR to S5-TIR. Since the inefficiency of soybean genetic transformation hinders the function verification of batch TIRs in SMV-resistance, the Nicotiana benthamiana-chimeric-SMV and N. benthamiana-pSMV-GUS pathosystems combined with Agrobacterium-mediated transient expression assays were invented and used to test the efficacy of these TIRs. From that, S1-TIR assembled from 462 bp CTF-S1 with 92% conservation rate performed its best on inhibiting SMV multiplication. Accordingly, S1-TIR was transformed into SMV-susceptible soybean NN1138-2, the resistant-healthy transgenic T1-plants were then picked out via detached-leaf inoculation assay with the stock-plants continued for progeny reproduction (T1 dual-utilization). All the four T3 transgenic progenies showed immunity to all the inoculated 11 SMV strains under individual or mixed inoculation, achieving a strong BSR. Thus, optimizing target for HIGS via transient N. benthamiana-chimeric-SMV and N. benthamiana-pSMV-GUS assays is crucial to drive robust resistance to SMV in soybean and the transgenic S1-TIR-lines will be a potential breeding source for SMV control in field.

Characterization of Resistance to Soybean mosaic virus in Diverse Soybean Germplasm

Crop Science ◽  
2005 ◽  
Vol 45 (6) ◽  
pp. 2503-2509 ◽  
Author(s):  
C. Zheng ◽  
P. Chen ◽  
R. Gergerich
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Germplasm

Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars

2015 ◽  
Vol 128 (8) ◽  
pp. 1489-1505 ◽  
Author(s):  
Le Gao ◽  
Xueni Ding ◽  
Kai Li ◽  
Wenlin Liao ◽  
Yongkun Zhong ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Inverted Repeat ◽  
Soybean Mosaic Virus ◽  
Soybean Cultivars
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