scholarly journals Optimizing RNAi-Target by Nicotiana benthamiana-Soybean Mosaic Virus System Drives Broad Resistance to Soybean Mosaic Virus in Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Hua Jiang ◽  
Kai Li ◽  
Junyi Gai
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Soybean Mosaic Virus ◽  
Multiple Resistance ◽  
Genome Sequences ◽  
Multiple Sequence ◽  
Detached Leaf ◽  
Broad Spectrum Resistance ◽  
Inoculation Assay ◽  
Virus System

Soybean mosaic virus (SMV) is a prevalent pathogen of soybean (Glycine max). Pyramiding multiple SMV-resistance genes into one individual is tedious and difficult, and even if successful, the obtained multiple resistance might be broken by pathogen mutation, while targeting viral genome via host-induced gene silencing (HIGS) has potential to explore broad-spectrum resistance (BSR) to SMV. We identified five conserved target fragments (CTFs) from S1 to S5 using multiple sequence alignment of 30 SMV genome sequences and assembled the corresponding target-inverted-repeat constructs (TIRs) from S1-TIR to S5-TIR. Since the inefficiency of soybean genetic transformation hinders the function verification of batch TIRs in SMV-resistance, the Nicotiana benthamiana-chimeric-SMV and N. benthamiana-pSMV-GUS pathosystems combined with Agrobacterium-mediated transient expression assays were invented and used to test the efficacy of these TIRs. From that, S1-TIR assembled from 462 bp CTF-S1 with 92% conservation rate performed its best on inhibiting SMV multiplication. Accordingly, S1-TIR was transformed into SMV-susceptible soybean NN1138-2, the resistant-healthy transgenic T1-plants were then picked out via detached-leaf inoculation assay with the stock-plants continued for progeny reproduction (T1 dual-utilization). All the four T3 transgenic progenies showed immunity to all the inoculated 11 SMV strains under individual or mixed inoculation, achieving a strong BSR. Thus, optimizing target for HIGS via transient N. benthamiana-chimeric-SMV and N. benthamiana-pSMV-GUS assays is crucial to drive robust resistance to SMV in soybean and the transgenic S1-TIR-lines will be a potential breeding source for SMV control in field.

scholarly journals Screening Isolates of Soybean mosaic virus for Infectivity in a Model Plant, Nicotiana benthamiana

Plant Disease ◽  
2015 ◽  
Vol 99 (4) ◽  
pp. 442-446 ◽  
Author(s):  
L. Gao ◽  
R. Zhai ◽  
Y. K. Zhong ◽  
A. Karthikeyan ◽  
R. Ren ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Soybean Mosaic Virus ◽  
Narrow Host Range ◽  
Model Plant ◽  
Resistance Strategies ◽  
The Family ◽  
New Hosts ◽  
Soybean Leaves

Soybean mosaic virus (SMV), belonging to the genus Potyvirus of the family Potyviridae, has a relatively narrow host range almost exclusively confined to leguminous hosts. While disease management through genetic transformation can be an effective approach, soybean remains recalcitrant to routine genetic transformation. In this context, it is important to identify new hosts for SMV that can be used to develop effective transgenic resistance strategies. Transformation in Nicotiana benthamiana is simple and highly efficient; hence, here we demonstrate the infectivity of SMV strain SC7 in N. benthamiana plants. To identify an SMV strain infectious in N. benthamiana, we mechanically inoculated N. benthamiana plants with 37 isolates from 21 (SC1 to SC21) SMV strains. Plants inoculated with isolates of strain SC7 produced mosaic symptoms on leaves. However, N. benthamiana plants inoculated with the 20 other SMV strains showed no visible symptoms. Furthermore, soybean cv. Nannong 1138-2 inoculated with sap prepared from symptomatic N. benthamiana leaves showed typical SMV mosaic symptoms 2 weeks after inoculation. In addition, SMV was detected in symptomatic N. benthamiana and soybean leaves by RT-PCR, DAS-ELISA, and further identified by sequencing. Together, the results indicate that N. benthamiana plants could support multiplication of SMV strain SC7. The findings of this study would be useful for the investigation of SMV resistance using the model plant N. benthamiana.

scholarly journals Complete Genome Sequences of the Genomic RNA of Soybean mosaic virus Strains G7B and G5

2003 ◽  
Vol 19 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Kook-Hyung Kim ◽  
Won-Seok Lim ◽  
Yul-Ho Kim
Keyword(s):  
Mosaic Virus ◽  
Complete Genome ◽  
Soybean Mosaic Virus ◽  
Genomic Rna ◽  
Genome Sequences ◽  
Virus Strains

scholarly journals Identification of a New Genetic Clade of Cowpea Mild Mottle Virus and Characterization of Its Interaction With Soybean Mosaic Virus in Co-infected Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhongyan Wei ◽  
Chenyang Mao ◽  
Chong Jiang ◽  
Hehong Zhang ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
High Throughput Sequencing ◽  
Soybean Mosaic Virus ◽  
Mottle Virus ◽  
Genome Sequences ◽  
Genomic Rnas ◽  
Disease Symptoms ◽  
Three Samples ◽  
Soybean Plants

Cowpea mild mottle virus (CPMMV; genus Carlavirus) can be a destructive pathogen of soybean but there is little information about its distribution on soybean in China. Here, we collected soybean plants with virus-like symptoms from 11 fields widely scattered within China, and used high-throughput sequencing to determine their virome. Most samples (8/11) were co-infected by the well-studied potyvirus soybean mosaic virus (SMV) and CPMMV, and the remaining three samples were singly infected with CPMMV. The near-complete genome sequences of the 11 CPMMV isolates were determined and phylogenetic analysis showed that they constituted a new genetic clade. One recombination event was detected among the CPMMV sequences, and the isolate CPMMV_JL_CC was identified as recombinant. In mechanical inoculation assays, co-infection by CPMMV and SMV resulted in an enhancement of disease symptoms, but decreased the expression level of the genomic RNAs and CP of CPMMV, without significantly affecting SMV accumulation. The interaction between these viruses needs further investigation.

Characterization of broad-spectrum resistance to Soybean mosaic virus in soybean [Glycine max (L.) Merr.] cultivar ‘RN-9’

2018 ◽  
Vol 137 (4) ◽  
pp. 605-613 ◽  
Author(s):  
Rui Ren ◽  
Jinlong Yin ◽  
Huanfang Zheng ◽  
Tao Wang ◽  
Shichao Liu ◽  
...  
Keyword(s):  
Glycine Max ◽  
Mosaic Virus ◽  
Broad Spectrum ◽  
Soybean Mosaic Virus ◽  
Broad Spectrum Resistance ◽  
Glycine Max L

scholarly journals Molecular variability and genetic structure of the population of Soybean mosaic virus based on the analysis of complete genome sequences

Virology ◽  
2009 ◽  
Vol 393 (1) ◽  
pp. 91-103 ◽  
Author(s):  
Jang-Kyun Seo ◽  
Kazusato Ohshima ◽  
Hyeok-Geun Lee ◽  
Moonil Son ◽  
Hong-Soo Choi ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mosaic Virus ◽  
Complete Genome ◽  
Soybean Mosaic Virus ◽  
Genome Sequences ◽  
Molecular Variability

Evaluation of germ plasm for resistant to Soybean mosaic virus (SMV)

2020 ◽  
Vol 21 (1) ◽  
pp. 6-9
Author(s):  
Wuye Ria Andayanie
Keyword(s):  
Abiotic Stress ◽  
Environmental Factors ◽  
Mosaic Virus ◽  
Polyclonal Antibody ◽  
Symptom Severity ◽  
Germ Plasm ◽  
Polyclonal Antibodies ◽  
Soybean Mosaic Virus ◽  
High Yield ◽  
High Yields

Soybean superior varieties with high yields and are resistant to abiotic stress have been largely released, although some varieties grown in the field are not resistant to SMV. In addition, the opportunity to obtain lines of hope as prospective varieties with high yield and resistance to SMV is very small. The method for evaluating soybean germplasm is based on serological observations of 98 accessions of leaf samples from SMV inoculation with T isolate. The evaluation results of 98 accessions based on visual observations showed 31 genotypes reacting very resistant or healthy to mild resistant category to SMV T isolate  with a percentage of symptom severity of 0 −30 %. Among 31 genotypes there are 2 genotypes (PI 200485; M8Grb 44; Mlg 3288) with the category of visually very resistant and resistant, respectively and  Mlg 3288  with the category of mild resistant.  They have a good agronomic appearance with a weight of 100 seeds (˃10 g) and react negatively with polyclonal antibodies to SMV, except Mlg 3288 reaction is not consistent, despite the weight of 100 seeds (˃ 10 g). Leaf samples from 98 accessions revealed various symptoms of SMV infection in the field. This diversity of symptoms is caused by susceptibility to accession, when infection occurs, and environmental factors. Keywords—: soybean; genotipe; Soybean mosaic virus (SMV); disease severity; polyclonal  antibody

Origin Analysis of Resistance Gene to Soybean Mosaic Virus in Soybean Line ICGR95-5117

2010 ◽  
Vol 36 (4) ◽  
pp. 549-554
Author(s):  
Rong-Xia GUAN ◽  
Yu-Bo CHEN ◽  
Hong-Liang FANG ◽  
Shuo LIU ◽  
Wei-Li TENG ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Soybean Mosaic Virus ◽  
Soybean Line ◽  
Origin Analysis

Inheritance of Reaction to Soybean Mosaic Virus in Two Soybean Cultivars

Crop Science ◽  
1989 ◽  
Vol 29 (6) ◽  
pp. 1439-1441 ◽  
Author(s):  
G. R. Buss ◽  
C. W. Roane ◽  
S. A. Tolin ◽  
P. Chen
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Cultivars
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