Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1

2018 ◽  
Vol 163 (6) ◽  
pp. 1549-1557 ◽  
Author(s):  
Greice Japolla ◽  
Jair Pereira Cunha-Junior ◽  
Ana Claudia Arantes Marquez Pajuaba ◽  
Ernesto Akio Taketomi ◽  
Samira Bührer-Sékula ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Inhibition Assay ◽  
Slot Blot ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 1

scholarly journals Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

2017 ◽  
Vol 130 ◽  
pp. 21-27
Author(s):  
Bianca S. Siedler ◽  
Bárbara C. Roloff ◽  
Gizele L. de Sá ◽  
Alessandra Neis ◽  
Fabrício R. Conceição ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Pichia Pastoris ◽  
Bovine Herpesvirus ◽  
Infected Cattle ◽  
Secretory Expression ◽  
Glycoprotein E ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 1

The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport

2011 ◽  
Vol 152 (3-4) ◽  
pp. 270-279 ◽  
Author(s):  
S.I. Chowdhury ◽  
M.C.S. Brum ◽  
C. Coats ◽  
A. Doster ◽  
Huiyong Wei ◽  
...  
Keyword(s):  
Axonal Transport ◽  
Envelope Protein ◽  
Bovine Herpesvirus ◽  
Herpesvirus Type ◽  
Acidic Domain ◽  
Anterograde Axonal Transport ◽  
Bovine Herpesvirus Type 1

scholarly journals Characterization of Monoclonal Antibodies against Bovine herpesvirus type 1 selected by Phage Display Technology

2017 ◽  
Vol 38 (6) ◽  
pp. 3915
Author(s):  
Greice Japolla ◽  
Ana Flávia Batista Penido ◽  
Greyciele Rodrigues Almeida ◽  
Luiz Artur Mendes Bataus ◽  
Jair Pereira Cunha Junior ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Bovine Herpesvirus ◽  
Single Chain ◽  
Phage Display Technology ◽  
Herpesvirus Type ◽  
Wide Range ◽  
Display Technology ◽  
Bovine Herpesvirus Type 1

The specificity of monoclonal antibodies (mAbs) to desired targets makes these molecules suitable for therapeutic and diagnostic uses against a wide range of pathogens. Phage display antibody libraries offer one method by which mAbs can be selected for, without the use of conventional hybridoma technology. In this work, phage display technology was used to construct, select and characterize a combinatorial single chain fragment variable (scFv) antibody library against bovine herpesvirus type 1 (BoHV-1) from the immune repertoire of chickens immunized with the virus. In silico analysis of the hypervariable domains of the antibody heavy chains revealed a high frequency of scFv fragments with low variability, suggesting that selection had probably been carried out and favored by a few im-munogenic viral antigens. The reactivity of the scFv fragments selected against BoHV-1 was demon-strated by Phage-ELISA. A significant increase in antibody reactivity to the target was observed after six rounds of library selection, showing its potential use as a molecule for BoHV-1 diagnosis. The strategy described here opens up a field for the use of phage display as a tool for selection of mono-clonal antibodies that could be used for theranostic applications against infectious and parasitic dis-eases of veterinary interest.

Effectiveness of a BHV-1/BEFV bivalent vaccine against bovine herpesvirus type 1 infection in cattle

2016 ◽  
Vol 109 ◽  
pp. 161-165 ◽  
Author(s):  
Yao-Chi Chung ◽  
Hsiu-Yen Shen ◽  
Li-Ting Cheng ◽  
Shyh-Shyan Liu ◽  
Chun-Yen Chu
Keyword(s):  
Bovine Herpesvirus ◽  
Bivalent Vaccine ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 1

scholarly journals Detection of antibodies against bovine respiratory syncytial virus (BRSV) in dairy cattle with different prevalences of bovine herpesvirus type 1 (BoHV-1) in São Paulo State, Brazil

2011 ◽  
Vol 32 (1) ◽  
pp. 295 ◽  
Author(s):  
Ingrid Bortolin Affonso ◽  
Sandra Possebon Gatti ◽  
Bruna Alexandrino ◽  
Mônica Costa Oliveira ◽  
Andréa Souza Ramos de Medeiros ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Dairy Cattle ◽  
Bovine Herpesvirus ◽  
Sao Paulo ◽  
Bovine Respiratory Syncytial Virus ◽  
Herpesvirus Type ◽  
Paulo State ◽  
Bovine Herpesvirus Type 1 ◽  
Syncytial Virus

Dynamics of bovine herpesvirus type 1 infection in Estonian dairy herds with and without a control programme

2012 ◽  
Vol 171 (4) ◽  
pp. 99-99 ◽  
Author(s):  
K. Raaperi ◽  
A. Aleksejev ◽  
T. Orro ◽  
A. Viltrop
Keyword(s):  
Bovine Herpesvirus ◽  
Control Programme ◽  
Dairy Herds ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 1
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