Genetic characterization of a novel G9P[23] rotavirus A strain identified in southwestern China with evidence of a reassortment event between human and porcine strains

2019 ◽  
Vol 164 (4) ◽  
pp. 1229-1232
Author(s):  
Danyu Chen ◽  
Long Zhou ◽  
Yiming Tian ◽  
Xuan Wu ◽  
Lan Feng ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Southwestern China ◽  
Reassortment Event ◽  
Rotavirus A

scholarly journals Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014)

2018 ◽  
Vol 33 (4) ◽  
pp. 304-313 ◽  
Author(s):  
Victoria Kiseleva ◽  
Evgeny Faizuloev ◽  
Elena Meskina ◽  
Anna Marova ◽  
Alexey Oksanich ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Rotavirus A ◽  
Human Rotavirus ◽  
Molecular Genetic Characterization

Genetic Characterization of Toxoplasma gondii Isolates From Pigs in Southwestern China

2011 ◽  
Vol 97 (6) ◽  
pp. 1193-1195 ◽  
Author(s):  
Peng Zhou ◽  
Xiu-Tao Sun ◽  
Chuang-Cheng Yin ◽  
Jian-Fa Yang ◽  
Zi-Guo Yuan ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Southwestern China ◽  
Toxoplasma Gondii Isolates

scholarly journals Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

2019 ◽  
Author(s):  
XinLei WANG ◽  
Ling DONG ◽  
Li ZHANG ◽  
Yan LV ◽  
Qian LI ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Genetic Characterization ◽  
Sichuan Province ◽  
Southwestern China ◽  
Wild Rodents ◽  
New Genotype ◽  
Pcr Rflp

Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

scholarly journals Genetic characterization of Toxoplasma gondii from cats in Yunnan Province, Southwestern China

2014 ◽  
Vol 7 (1) ◽  
pp. 178 ◽  
Author(s):  
Yi-Ming Tian ◽  
Si-Yang Huang ◽  
Qiang Miao ◽  
Hai-Hai Jiang ◽  
Jian-Fa Yang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Yunnan Province ◽  
Genetic Characterization ◽  
Southwestern China

scholarly journals Non-structural Enterotoxin (NSP4) Gene based Molecular Characterization of Caprine and Ovine Rotavirus A, India

2020 ◽  
Vol 14 (4) ◽  
pp. 2303-2311
Author(s):  
Shubhankar Sircar ◽  
Prashant Kumar ◽  
Mohd Ikram Ansari ◽  
Sudipta Bhat ◽  
Jobin Jose Kattoor ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genetic Characterization ◽  
Full Length ◽  
Viral Gastroenteritis ◽  
Enterotoxin Gene ◽  
Nsp4 Gene ◽  
Future Studies ◽  
Rotavirus A ◽  
Large Animals

Rotavirus A (RVA) causes viral gastroenteritis in humans and animals, including calves, piglets, and foals. The current study reports the genetic characterization of the full-length enterotoxin gene, NSP4, from caprine and ovine species. Upon characterizing eight full-length NSP4 genes by sequencing, it was found that the four caprine and three ovine RVAs NSP4 genes are of E2 genotype and the sole ovine RVA isolate was found to be of E1 genotype. In the sequence and phyloanalysis of the NSP4 gene the seven E2 genotypes clustered with bovine, human, and caprine isolates from India and Bangladesh, respectively. The E1 genotype of ovine RVA was closer to human RVA isolate from India. The nucleotide per cent identity analysis revealed that all E2 genotype strains of caprine and ovine species ranged from 88.4% to 90.4% and it was found common to both the reference human RVA isolates DS-1 and AU-1. Whereas, the E1 genotype ovine strain clustered with human RVA isolates with 93.1% nucleotide per cent identity. The RVA strains circulating in caprine and ovine populations may share a common origin which is usually found in artiodactyl species because humans share a common dwelling with animals. Future studies are needed to confirm these findings of their relationship with humans and large animals.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

Clinical and genetic characterization of two cases of central hypogonadism in Klinefelter syndrome

2020 ◽  
Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Klinefelter Syndrome
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