Establishment of an enzyme-linked immunosorbent assay for Getah virus infection in horses using a 20-mer synthetic peptide for the E2 glycoprotein as an antigen

2019 ◽  
Vol 165 (2) ◽  
pp. 377-385
Author(s):  
Hiroshi Bannai ◽  
Manabu Nemoto ◽  
Koji Tsujimura ◽  
Minoru Ohta
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Synthetic Peptide ◽  
Enzyme Linked Immunosorbent Assay ◽  
E2 Glycoprotein ◽  
Getah Virus

Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen

2019 ◽  
Vol 271 ◽  
pp. 113681 ◽  
Author(s):  
Hiroshi Bannai ◽  
Manabu Nemoto ◽  
Koji Tsujimura ◽  
Takashi Yamanaka ◽  
Hiroshi Kokado
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
E2 Protein ◽  
Getah Virus

scholarly journals A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

2019 ◽  
Vol 6 (3) ◽  
pp. 64
Author(s):  
Miki Okumura ◽  
Akiko Takenaka-Uema ◽  
Shin Murakami ◽  
Taisuke Horimoto
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Influenza A ◽  
Influenza Virus Infection ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pandemic Preparedness ◽  
Influenza A Viruses ◽  
Diagnosis Method ◽  
Influenza A Virus Infection

Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.

Seroprevalence of Nipah Virus Infection in Peninsular Malaysia

2020 ◽  
Vol 221 (Supplement_4) ◽  
pp. S370-S374
Author(s):  
Ming-Yen Yong ◽  
Soo-Ching Lee ◽  
Romano Ngui ◽  
Yvonne Ai-Lian Lim ◽  
Maude E Phipps ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Indigenous Population ◽  
Nipah Virus ◽  
Peninsular Malaysia ◽  
Natural Host ◽  
Enzyme Linked Immunosorbent Assay ◽  
N Protein ◽  
Reservoir Species ◽  
Host Reservoir

Abstract Nipah virus (NiV) outbreak occurred in Malaysia in 1998. The natural host reservoir for NiV is Pteropus bats, which are commonly found throughout Malaysia. Humans become infected when NiV spills over from the reservoir species. In this study, NiV serosurveillance in Peninsular Malaysia, particularly among the indigenous population, was performed. The collected samples were tested for presence of NiV antibodies using a comparative indirect enzyme-linked immunosorbent assay based on the recombinant NiV nucleocapsid (rNiV-N) protein. We found that 10.73% of the participants recruited in this study had antibodies against rNiV-N, suggesting possible exposure to NiV.

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