Enzyme-linked immunosorbent assay in the diagnosis of Kilham rat virus infection in rats

1984 ◽  
Vol 18 (4) ◽  
pp. 364-370 ◽  
Author(s):  
S. B. Singh ◽  
C. M. Lang
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Kilham Rat Virus

scholarly journals A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

2019 ◽  
Vol 6 (3) ◽  
pp. 64
Author(s):  
Miki Okumura ◽  
Akiko Takenaka-Uema ◽  
Shin Murakami ◽  
Taisuke Horimoto
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Influenza A ◽  
Influenza Virus Infection ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pandemic Preparedness ◽  
Influenza A Viruses ◽  
Diagnosis Method ◽  
Influenza A Virus Infection

Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.

Seroprevalence of Nipah Virus Infection in Peninsular Malaysia

2020 ◽  
Vol 221 (Supplement_4) ◽  
pp. S370-S374
Author(s):  
Ming-Yen Yong ◽  
Soo-Ching Lee ◽  
Romano Ngui ◽  
Yvonne Ai-Lian Lim ◽  
Maude E Phipps ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Indigenous Population ◽  
Nipah Virus ◽  
Peninsular Malaysia ◽  
Natural Host ◽  
Enzyme Linked Immunosorbent Assay ◽  
N Protein ◽  
Reservoir Species ◽  
Host Reservoir

Abstract Nipah virus (NiV) outbreak occurred in Malaysia in 1998. The natural host reservoir for NiV is Pteropus bats, which are commonly found throughout Malaysia. Humans become infected when NiV spills over from the reservoir species. In this study, NiV serosurveillance in Peninsular Malaysia, particularly among the indigenous population, was performed. The collected samples were tested for presence of NiV antibodies using a comparative indirect enzyme-linked immunosorbent assay based on the recombinant NiV nucleocapsid (rNiV-N) protein. We found that 10.73% of the participants recruited in this study had antibodies against rNiV-N, suggesting possible exposure to NiV.

scholarly journals Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

2011 ◽  
Vol 18 (10) ◽  
pp. 1773-1775 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Ampai Tanganuchitcharnchai ◽  
Richard G. Jarman ◽  
Robert V. Gibbons ◽  
Daniel H. Paris ◽  
...  
Keyword(s):  
Virus Infection ◽  
Diagnostic Accuracy ◽  
Immunosorbent Assay ◽  
Chikungunya Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sri Lankan ◽  
Immunochromatographic Test

ABSTRACTA Sri Lankan fever cohort (n= 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

Sign in / Sign up

Export Citation Format

Share Document