Diagnosis of hepatitis C virus infection by enzyme-linked immunosorbent assay using synthetic peptide antigens

Peptides ◽  
1995 ◽  
pp. 266-268
Author(s):  
Bing Xu ◽  
Jun-Shen Zhan ◽  
Qing Dong
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Immunosorbent Assay ◽  
Synthetic Peptide ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis C Virus Infection ◽  
Peptide Antigens

scholarly journals Quantification of Different Human Alpha Interferon Subtypes and Pegylated Interferon Activities by Measuring MxA Promoter Activation

2005 ◽  
Vol 49 (9) ◽  
pp. 3770-3775 ◽  
Author(s):  
Catherine François ◽  
Isabelle Bernard ◽  
Sandrine Castelain ◽  
Bryan Charleston ◽  
Martin D. Fray ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Biological Samples ◽  
Pegylated Interferon ◽  
Biologically Active ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis C Virus Infection ◽  
Promoter Activation ◽  
Antiviral Protein

ABSTRACT Alpha interferons (α-IFNs) are potent biologically active proteins synthesized and secreted by somatic cells during viral infection. Quantification of α-IFN concentrations in biological samples is used for diagnosis. More recently, recombinant IFNs have been used as antiviral, antiproliferative, and immunomodulatory therapeutic agents, and particularly for the treatment of chronic hepatitis C virus infection. For this purpose, IFN has recently been coupled to polyethylene glycol (PEG) to improve the pharmacokinetic properties. The measure of α-IFN in biological samples from treated patients could be useful to ensure compliance to therapy and the true IFN activity in relation to viral decay during follow-up. In particular, it could be used to monitor the PEG-IFN concentration in patients treated for hepatitis C virus infection. The most frequently used test is a bioassay based on the antiviral property of the IFN, but the assay is not highly reproducible. Here, we present a reporter test based on MxA promoter activation of chloramphenicol acetyltransferase expression (Mx-CAT). MxA is an antiviral protein induced and tightly regulated by α-IFN. The Mx-CAT assay showed good reproducibility of 15% and was suitable to quantify PEG-IFN and numerous other α-IFN subtypes as well, despite a differential MxA promoter activation in relation with the subtype. A good correlation was obtained with the reporter assay and a commercial enzyme-linked immunosorbent assay on samples from treated patients. This test could be useful for monitoring IFN therapy of chronically infected hepatitis C virus-infected patients treated with the standard IFN, PEG-IFN, and probably forthcoming recombinant IFNs.

scholarly journals Detection of acute hepatitis C virus infection by ELISA using a synthetic peptide comprising a structural epitope.

1992 ◽  
Vol 89 (10) ◽  
pp. 4486-4489 ◽  
Author(s):  
G. J. Kotwal ◽  
B. M. Baroudy ◽  
I. K. Kuramoto ◽  
F. F. McDonald ◽  
G. M. Schiff ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Acute Hepatitis ◽  
Synthetic Peptide ◽  
Acute Hepatitis C ◽  
Hepatitis C Virus Infection

scholarly journals Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein.

1991 ◽  
Vol 88 (9) ◽  
pp. 3647-3651 ◽  
Author(s):  
B. Hosein ◽  
C. T. Fang ◽  
M. A. Popovsky ◽  
J. Ye ◽  
M. Zhang ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Capsid Protein ◽  
Synthetic Peptide ◽  
Hepatitis C Virus Infection ◽  
Peptide Antigen

scholarly journals Frequency of Hepatitis C Virus in Diagnosed Tuberculosis Cases

2021 ◽  
Vol 15 (5) ◽  
pp. 1041-1043
Author(s):  
Muhammad Farooq Baig ◽  
Geeta Kumari ◽  
Amir Bux Detho ◽  
Abdul Saleem Qadeer ◽  
Ball Chand ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immunosorbent Assay ◽  
Rapid Test ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis C Virus Infection ◽  
Chain Reaction ◽  
Polymerase Chain

Background: The frequency of hepatitis C virus infection along with tuberculosis has not been widely investigated and very low statistics on rates of hepatitis C virus co-infection in tuberculosis patients. Hepatotoxicity is the major side effect of anti-tuberculosis therapy hepatitis HCVliver disease elevates the chances of hepatotoxicity up-to five folds. Aim: To see the frequency of Hepatitis Cvirus infection amongst people with diagnosed Tuberculosis using gene X-pert technique. To evaluate the factors associated with HCVinfection in patients with MTBtuberculosis and to determine sensitivity and specificity of the tests. Study design: Comparative analytical study. Place and duration of study: Pathology Department, Civil Hospital Mirpur Khas Sindh from 1stJanuary 2017 to 31st December 2018. Methodology: Three hundred and thirteen patients of tuberculosis diagnosed by Genexpert included while testing hepatitis C virus using immunochromotography rapid test technique, enzyme linked immunosorbent assay method and polymerase chain reaction test for confirmation. Results:Higher frequency of tuberculosis infection in males 57.8%, 42.5% between 20-39 years and 22% of hepatitis C virus infection in tuberculosis patients.Sensitivity of rapid test and enzyme-linked immunosorbent assay was 79% and 96% respectively while the specificity of rapid test and enzyme-linked immunosorbent assay was 91% and 99% respectively. Conclusion: The high frequency of hepatitis C virus co-infection was found among tuberculosis cases in Mirpur Khas Division Sindh. Enzyme-linked immunosorbent assay method is more accurate, reliable as compared to rapid immunochromatographytest for hepatitis C virus and polymerase chain reaction is still gold standard. Keywords: TB, Hepatitis C virus, Mycobacterium tuberculosis,, PCR, Genexpert, Rapid test

scholarly journals Prevalence of Antibody to Hepatitis C Virus in an Isolated Canadian Inuit Settlement

1991 ◽  
Vol 2 (2) ◽  
pp. 71-73
Author(s):  
GY Minuk ◽  
LE Nicolle ◽  
T Gauthier ◽  
J Brunka
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Hepatitis A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis C Virus Infection ◽  
Increased Risk ◽  
Donor Population ◽  
Baker Lake ◽  
Virus Exposure

Sera from 720 inhabitants of Baker Lake, Northwest Territories, a community with high rates of hepatitis A and B infection, were tested for antibody to hepatitis C virus by commercial enzyme-linked immunosorbent assay. Only two individuals (0.3%) were positive, a 63-year-old female and an unrelated 10-year-old male. Neither individual was at increased risk of hepatitis C virus exposure. The results of this study indicate that hepatitis C virus infection is no more common in this northern Canadian Inuit settlement than it is in the blood donor population of southern Canada.

Pyoderma gangrenosum and hepatitis C virus infection

1998 ◽  
Vol 139 (5) ◽  
pp. 924-925 ◽  
Author(s):  
Keane ◽  
Macfarlane ◽  
Munn ◽  
Higgins
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Pyoderma Gangrenosum ◽  
Hepatitis C Virus Infection

HEPATITIS C VIRUS INFECTION (AND ADDITIONAL NEOPLASMS) AMONG MARGINAL ZONE LYMPHOMAS

1997 ◽  
Vol 96 (2) ◽  
pp. 427-428 ◽  
Author(s):  
FREDERICO SILVESTRI ◽  
GIOVANNI BARILLARI ◽  
RENATO FANIN ◽  
FLAVIA SALMASO ◽  
LAURA INFANTI ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Marginal Zone ◽  
Hepatitis C Virus Infection
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