scholarly journals Sugarcane mosaic virus remodels multiple intracellular organelles to form genomic RNA replication sites

2021 ◽  
Author(s):  
Jipeng Xie ◽  
Tong Jiang ◽  
Zhifang Li ◽  
Xiangdong Li ◽  
Zaifeng Fan ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Rna Replication ◽  
Sugarcane Mosaic Virus ◽  
Genomic Rna ◽  
Replication Sites ◽  
Intracellular Organelles

scholarly journals Sugarcane Mosaic Virus Remodels Multiple Intracellular Organelles to Form Genomic RNA Replication Sites

2021 ◽  
Author(s):  
Jipeng Xie ◽  
Tong Jiang ◽  
Zhifang Li ◽  
Xiangdong Li ◽  
Zaifeng Fan ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Crop Production ◽  
Rna Viruses ◽  
Rna Replication ◽  
Sugarcane Mosaic Virus ◽  
Genome Replication ◽  
Genomic Rna ◽  
Range Analysis ◽  
Viral Genome Replication ◽  
Intracellular Organelles

Abstract Positive-stranded RNA viruses usually remodel host endomembrane system to form virus-induced intracellular vesicles for replication during infections. The genus Potyvirus of Potyviridae represents the largest number of positive single-stranded RNA viruses and causes great damage on crop production worldwide. Though potyviruses have wide host ranges, each potyvirus infects relatively limited host species. Phylogenesis and host range analysis can divide potyviruses into monocot-infecting and dicot-infecting groups, suggesting that some infection mechanism, probably on replication may be distinct for each group. Comprehensive studies on the model dicot-infecting turnip mosaic virus indicated that the 6K2-induced replication vesicles are derived from endoplasmic reticulum (ER) and subsequently target chloroplasts for viral genome replication. However, we have no knowledge on the replication site of monocot-infecting potyviruses. In this study, we firstly show that the precursor 6K2-VPg-Pro polyproteins of dicot-infecting potyviruses and monocot-infecting potyviruses phylogenetically cluster in two separate groups. With a typical gramineae-infecting potyvirus sugarcane mosaic virus (SCMV), we found that SCMV replicative double-stranded RNA (dsRNA) forms aggregates in cytoplasm but does not associate with chloroplasts. SCMV 6K2-VPg-Pro-induced vesicles colocalize with replicative dsRNA. Moreover, SCMV 6K2-VPg-Pro-induced structures target multiple intracellular organelles including ER, Golgi apparatus, mitochondria and peroxisomes, and have no evident association with chloroplasts. In conclusion, SCMV remodels multiple intracellular organelles for its genomic RNA replication.

scholarly journals Yeast Lsm1p-7p/Pat1p Deadenylation-Dependent mRNA-Decapping Factors Are Required for Brome Mosaic Virus Genomic RNA Translation

2003 ◽  
Vol 23 (12) ◽  
pp. 4094-4106 ◽  
Author(s):  
Amine O. Noueiry ◽  
Juana Diez ◽  
Shaun P. Falk ◽  
Jianbo Chen ◽  
Paul Ahlquist
Keyword(s):  
Mosaic Virus ◽  
Rna Replication ◽  
Brome Mosaic Virus ◽  
Open Reading Frame ◽  
Viral Rna ◽  
Genomic Rna ◽  
Reading Frame ◽  
Mrna Decapping ◽  
Rna Translation ◽  
Positive Strand Rna

ABSTRACT Previously, we used the ability of the higher eukaryotic positive-strand RNA virus brome mosaic virus (BMV) to replicate in yeast to show that the yeast LSM1 gene is required for recruiting BMV RNA from translation to replication. Here we extend this observation to show that Lsm1p and other components of the Lsm1p-Lsm7p/Pat1p deadenylation-dependent mRNA decapping complex were also required for translating BMV RNAs. Inhibition of BMV RNA translation was selective, with no effect on general cellular translation. We show that viral genomic RNAs suitable for RNA replication were already distinguished from nonreplication templates at translation, well before RNA recruitment to replication. Among mRNA turnover pathways, only factors specific for deadenylated mRNA decapping were required for BMV RNA translation. Dependence on these factors was not only a consequence of the nonpolyadenylated nature of BMV RNAs but also involved the combined effects of the viral 5′ and 3′ noncoding regions and 2a polymerase open reading frame. High-resolution sucrose density gradient analysis showed that, while mutating factors in the Lsm1p-7p/Pat1p complex completely inhibited viral RNA translation, the levels of viral RNA associated with ribosomes were only slightly reduced in mutant yeast. This polysome association was further verified by using a conditional allele of essential translation initiation factor PRT1, which markedly decreased polysome association of viral genomic RNA in the presence or absence of an LSM7 mutation. Together, these results show that a defective Lsm1p-7p/Pat1p complex inhibits BMV RNA translation primarily by stalling or slowing the elongation of ribosomes along the viral open reading frame. Thus, factors in the Lsm1p-7p/Pat1p complex function not only in mRNA decapping but also in translation, and both translation and recruitment of BMV RNAs to viral RNA replication are regulated by a cell pathway that transfers mRNAs from translation to degradation.

scholarly journals Maize resistance to Sugarcane mosaic virus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 542-544
Author(s):  
R. Pokorný ◽  
M. Porubová
Keyword(s):  
Mosaic Virus ◽  
Systemic Infection ◽  
Sugarcane Mosaic Virus ◽  
Resistant Line ◽  
Mechanisms Of Resistance ◽  
Long Distance ◽  
Maize Hybrids ◽  
Resistant Lines ◽  
Maize Resistance ◽  
Long Distance Movement

Under greenhouse conditions 12 maize hybrids derived from crosses of four resistant lines with several lines of different level of susceptibility were evaluated for resistance to Czech isolate of Sugarcane mosaic virus (SCMV). These hybrids were not fully resistant to isolate of SCMV, but the symptoms on their newly growing leaves usually developed 1 to 3 weeks later in comparison with particular susceptible line, the course of infection was significantly slower and rate of infection lower. As for mechanisms of resistance, the presence of SCMV was detected by ELISA in inoculated leaves both of resistant and susceptible lines, but virus was detected 7 days later in resistant line. Systemic infection developed only in susceptible lines. These results indicate restriction of viral long distance movement in the resistant line.

scholarly journals Virus reconstitution and the proof of the existence of genomic RNA

1999 ◽  
Vol 354 (1383) ◽  
pp. 583-586 ◽  
Author(s):  
H. Fraenkel-Conrat ◽  
B. Singer
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Biological Properties ◽  
Viral Rna ◽  
Historical Overview ◽  
Genomic Rna ◽  
Primary Finding ◽  
Work Done ◽  
Component Protein

This paper is a historical overview of the work done on the tobacco mosaic virus. The primary finding was that a virus is capable of reassembling itself from its component protein and RNA, and that only the RNA carries the genomic capability of the virus. This was followed by detailed studies of the chemical and biological properties of viral RNA.

scholarly journals The Complete Nucleotide Sequence of Bean Yellow Mosaic Virus Genomic RNA.

1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Genomic Rna

scholarly journals Variabilidade genética de Sugarcane mosaic virus, causando mosaico em milho no Brasil

2011 ◽  
Vol 46 (4) ◽  
pp. 362-369 ◽  
Author(s):  
Marcos Cesar Gonçalves ◽  
Diogo Manzano Galdeano ◽  
Ivan de Godoy Maia ◽  
César Martins Chagas
Keyword(s):  
Mosaic Virus ◽  
Sao Paulo ◽  
Sugarcane Mosaic Virus ◽  
Maize Dwarf Mosaic Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Das Elisa

O objetivo deste trabalho foi caracterizar biológica e molecularmente três isolados de Sugarcane mosaic virus (SCMV) de lavouras de milho, analisá-los filogeneticamente e discriminar polimorfismos do genoma. Plantas com sintomas de mosaico e nanismo foram coletadas em lavouras de milho, no Estado de São Paulo e no Município de Rio Verde, GO, e seus extratos foliares foram inoculados em plantas indicadoras e submetidos à análise sorológica com antissoros contra o SCMV, contra o Maize dwarf mosaic virus (MDMV) e contra o Johnsongrass mosaic virus (JGMV). Mudas de sorgo 'Rio' e 'TX 2786' apresentaram sintomas de mosaico após a inoculação dos três isolados, e o DAS-ELISA confirmou a infecção pelo SCMV. O RNA total foi extraído e usado para amplificação por transcriptase reversa seguida de reação em cadeia de polimerase (RT-PCR). Fragmentos específicos foram amplificados, submetidos à análise por polimorfismo de comprimento de fragmento de restrição (RFLP) e sequenciados. Foi possível discriminar os genótipos de SCMV isolados de milho de outros isolados brasileiros do vírus. Alinhamentos múltiplos e análises dos perfis filogenéticos corroboram esses dados e mostram diversidade nas sequências de nucleotídeos que codificam para a proteína capsidial, o que explica o agrupamento separado desses isolados e sugere sua classificação como estirpes distintas, em lugar de simples isolados geográficos.

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