The in vitro germination and storage characteristics of Keteleeria fortunei var. cyclolepis pollen provide a reference for cross breeding

An experiment for strawberry Fragaria ananassa cv. Festival in vitro conservation of cultures for nine months was studied. Three MS medium strength (full strength MS, ¾ MS, ½ MS, and ¼ MS) and the storage period (3, 6, and 9 months) in slowing down or reducing growth. Results showed a significant effect of the medium salt strength, as the full strength treatment was higher by achieved the highest percentage of viability (survival %) and storage characteristics of cultures (N%, P%, carbohydrates%, protein %) and regeneration ability characteristics (shoots and roots number and length). Three-month storage period achieved the highest rate compared to the nine-month storage period, which recorded the lowest average for the studied characteristics. The full strength treatment for three months storage period realized the highest rate compared to treatment ¼ strength for the storage period of nine months, which recorded the lowest rate of the studied traits. The application of minimal strength for in vitro conservation allows strawberry cultures to remain viable with a slow growth rate and a significant increase in the storage life of the tissues to nine months.

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In vitro germination protocol for the propagation of the endangered butternut tree (Juglans cinerea L.)

Native Plants Journal ◽

10.3368/npj.20.2.117 ◽

2019 ◽

Vol 20 (2) ◽

pp. 117-122 ◽

Cited By ~ 1

Author(s):

Martin Williams ◽

Kathleen Forbes ◽

Charlene Williams ◽

Tannis Beardmore

Keyword(s):

In Vitro Germination ◽

Juglans Cinerea

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IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN. X. POLLEN SOURCE GENOTYPE AND GIBBERELLIN A3INTERACTIONS*

Acta Botanica Neerlandica ◽

10.1111/j.1438-8677.1982.tb01596.x ◽

1982 ◽

Vol 31 (1-2) ◽

pp. 105-111 ◽

Cited By ~ 3

Author(s):

P. L. Pfahler ◽

M. Wilcox ◽

D. L. Mulcahy ◽

D. A. Knauft

Keyword(s):

Zea Mays ◽

Pollen Tube ◽

Pollen Tube Growth ◽

Zea Mays L ◽

Tube Growth ◽

In Vitro Germination

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Release of cyclic guanosine monophosphate evaluated as a diagnostic tool in cardiac diseases

Clinical Chemistry ◽

10.1093/clinchem/37.2.186 ◽

1991 ◽

Vol 37 (2) ◽

pp. 186-190 ◽

Cited By ~ 39

Author(s):

Karl-P Vorderwinkler ◽

Eilka Artner-Dworzak ◽

Gab Jakob ◽

Johanne Mair ◽

Franz Diensti ◽

...

Keyword(s):

Cyclic Guanosine Monophosphate ◽

Guanosine Monophosphate ◽

Cardiac Diseases ◽

Blood Samples ◽

Cutoff Value ◽

Intracellular Cgmp ◽

Glycerol Trinitrate ◽

Clinical Measurements ◽

And Storage

Abstract Concentrations of atrial natriuretic peptide (ANP) are increased in plasma of patients with impaired cardiac and renal function. The second messenger of ANP, cyclic guanosine monophosphate (cGMP), is released into the plasma specifically upon stimulation of cells with ANP. Although nitrates can also activate intracellular cGMP synthesis, we detected no increase in plasma cGMP concentrations after infusions of glycerol trinitrate. Because immunoreactive ANP is highly susceptible to degradation and nonspecific influences in blood samples, determinations of ANP require immediate centrifugation and storage of plasma at -20 degrees C. In contrast, we found that cGMP is stable for five days in vitro in blood samples containing EDTA. In 147 healthy blood donors, the upper cutoff value for plasma cGMP was 6.60 nmol/L, not significantly different (P greater than 0.05) from that for 222 patients with disorders other than cardiovascular and renal. In 69 patients with manifest congestive heart failure (NYHA stages II-IV), 65 had increased cGMP values. Using the above cutoff value for cGMP gave diagnostic sensitivity of 94.2% and specificity of 93.7%. Plasma cGMP may thus provide an alternative for routine clinical measurements of ANP in cardiac diseases in the absence of renal disorders.

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Effect of Domestic Processing and Cooking Methods on Total, HCl Extractable Iron and in vitro availability of Iron in Spinach and Amaranth Leaves

Nutrition and Health ◽

10.1177/026010600201600205 ◽

2002 ◽

Vol 16 (2) ◽

pp. 113-120 ◽

Cited By ~ 8

Author(s):

S.K. Yadav ◽

S. Sehgal

Keyword(s):

Iron Content ◽

Dry Weight ◽

Total Iron ◽

Cooking Methods ◽

In Vitro Availability ◽

Domestic Processing ◽

Polyethylene Bags ◽

And Storage ◽

Extractable Iron

Spinach ( Spinacia oleracia) and amaranth ( Amaranthus tricolor) leaves were stored in polyethylene bags and without packing for 24 and 48 hours in a refrigerator at 5°C and 30°C in polyethylene bags. The fresh leaves were also dried (oven and sun), blanched (5, 10 and 15 min) and cooked in an open pan and a pressure cooker. The processed leaves were analysed for total iron, its availability and antinutrient content. The iron content of these leaves varied from 26.54 to 34.14 mg/l00g, dry weight and its HCl-extractability and in vitro availability were 62.11–67.18% and 3.03–3.97% of total respectively. Drying and storage had no significant effect on total iron content, Hel-extractability and availability ( in vitro), while blanching and cooking resulted in significant improvement of iron availability, and a significant reduction in oxalic acid content, while only blanching significantly reduced phytic acid and polyphenol contents. Thus cooking and blanching are good ways to improve HCl-extractability and in vitro availability of iron.

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