scholarly journals Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

2021 ◽  
Vol 34 (2) ◽  
pp. 1-9
Author(s):  
Zaid K. Kadhim ◽  
Muslim A. A. Abdulhussein
Keyword(s):  
Storage Period ◽  
In Vitro Conservation ◽  
Regeneration Ability ◽  
Fragaria Ananassa ◽  
Slow Growth Rate ◽  
Slowing Down ◽  
Full Strength ◽  
And Storage ◽  
Storage Characteristics

An experiment for strawberry Fragaria ananassa cv. Festival in vitro conservation of cultures for nine months was studied. Three MS medium strength (full strength MS, ¾ MS, ½ MS, and ¼ MS) and the storage period (3, 6, and 9 months) in slowing down or reducing growth. Results showed a significant effect of the medium salt strength, as the full strength treatment was higher by achieved the highest percentage of viability (survival %) and storage characteristics of cultures (N%, P%, carbohydrates%, protein %) and regeneration ability characteristics (shoots and roots number and length). Three-month storage period achieved the highest rate compared to the nine-month storage period, which recorded the lowest average for the studied characteristics. The full strength treatment for three months storage period realized the highest rate compared to treatment ¼ strength for the storage period of nine months, which recorded the lowest rate of the studied traits. The application of minimal strength for in vitro conservation allows strawberry cultures to remain viable with a slow growth rate and a significant increase in the storage life of the tissues to nine months.

scholarly journals The relationship between the storage methods and the formation of dentinal defects (cracks)

2019 ◽  
Vol 67 ◽  
Author(s):  
Fábio Nakao ARASHIRO ◽  
Michelle Tavares Galotto NANTES ◽  
Pedro Gregol da SILVA ◽  
Key Fabiano Souza PEREIRA ◽  
Muryllo Eduardo Sales dos SANTOS
Keyword(s):  
Storage Period ◽  
Root Apex ◽  
Surgical Instruments ◽  
Distilled Water ◽  
Chi Square ◽  
Significance Level ◽  
Significant Difference ◽  
And Storage ◽  
Group 1

ABSTRACT Objective: This research study aims at conducting an in vitro evaluation of crack formation in freshly extracted teeth after undergoing different storage and decontamination methods. Methods: 60 erupted upper third molars conventionally extracted using forceps # 210h (quinelato surgical instruments, rio claro - sp) and randomly distributed in three groups (n = 30): group 1 - storage in dry environment for 30 days, group 2 - sterilization in autoclave and storage for 30 days in distilled water, and group 3 - 10% formaldehyde decontamination for 14 days and storage in distilled water for additional 30 days. after the storage period, teeth had their roots transversely sectioned at 2, 4 and 6 mm below the root apex using a low rotation diamond disk under constant cooling. the evaluation of fragments was performed using a 30-time magnification microscope. Results: Cracks were seen only in group 1 and the chi-square statistical test with 5% significance level showed a statistically significant difference comparing the dry storage group to the others. Conclusion: The storage of extracted teeth in a dry environment influences the formation of dentinal defects.

scholarly journals KONSERVASI TANAMAN LADA (Piper nigrum L.) SECARA IN VITRO

2020 ◽  
Vol 7 (3) ◽  
pp. 88 ◽  
Author(s):  
YELNITITIS YELNITITIS ◽  
NURLIANI BERMAWIE
Keyword(s):  
Block Design ◽  
Plant Genetic Resources ◽  
Black Pepper ◽  
In Vitro Conservation ◽  
Piper Nigrum ◽  
Regeneration Ability ◽  
Single Node ◽  
Shoot Height ◽  
Number Of Leaves

<p><strong>In vitro conservation of black pepper (Piper nigrum L.)</strong></p><p>Black pepper (Piper nigrum L.) is one of the economically im¬ potant spices. The major constraint in black pepper cultivation and conservation in ield is foot rot disease caused by Phytopthora capsici which could cause plants die. Conservation of black pepper germplasms as living collections in ield is risky due to pests and natural disaster. The experiment on in vitro cop ervation of black pepper var. LDL was conducted al the laboratory of Plant Genetic Resources and Breeding, Research Institute for Spice and Medicinal Crops (RISMC) Bogor from April 1998 to Maret 1999. Single node cuttings from sterile culture were used as explains. The explains were cultured on Murashige and Skoog (MS) medium on full and half strength concentration supplemented with paclobutrazol (paclo) (0, 1, 3 and 5 mg/1). The experiment was performed in a randomized complete block design arranged factorially with 10 replications. The result showed that the medium supplemented with paclo on both full MS and MS A medium could suppress vegetative growth until 12 months. There was no signiicant interaction between medium and paclo on shoot initiation. The effect was signiicant on shoot height, number of leaves and culture performances. Increasing paclo concen¬ tration caused higher suppression of plant growth. MS A medium supplemented with paclo 5 mg/1 showed the slowest growth with shool height 2.10 cm and number of leaves 9. Culture performance was fresh, with green leaves and vigorous. Advcntive shoots were able to regenerate on the medium supplemented with BA 0.3 mg/1. In vitro conservation of black pepper with paclo did not change plant regeneration ability. Therefore, this technique may be used as an altenative method for black pepper conservation.</p>

scholarly journals Effect of Polymerization Time on Residual Monomer Release in Dental Composite: In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Ezgi Sonkaya ◽  
Zeliha Gonca Bek Kürklü ◽  
Şeyhmus Bakır
Keyword(s):  
High Performance ◽  
Storage Period ◽  
In Vitro Study ◽  
Composite Resins ◽  
Dental Composite ◽  
Polymerization Time ◽  
Residual Monomer ◽  
Significant Difference ◽  
And Storage

Light activated resin-based composites are the most accepted and used materials among clinicians. The aim of this study is to determine the amount of residual monomer released from nanofiller composite resins for different polymerization times and storage periods in vitro. To this purpose, Tetric Ceram (Ivoclar, Liechtenstein), Clearfil Majesty Posterior (Kuraray, Japan), Grandio (VOCO, Germany), and Filtek Ultimate Universal (3M, USA) were used as nanofiller resin composites samples. Four groups ( n = 40 , diameter: 5 mm, thickness: 2 mm) of each material were fabricated, and each group was exposed to three different polymerization time (10, 20 and 40 sec). High-performance liquid chromatography (HPLC) was used to measure the amount of monomers released over 1, 15, and 30 days. The highest amount of monomer release was seen in Tetric EvoCream composite, while the least monomer release was seen in Clearfil Majesty composite. Regardless of the polymerization time, material, or storage period, the highest amount of eluted monomer was Bis-GMA. It is observed that there is no statistically significant difference between various polymerization times. Monomer release reached its highest level on the 15th day and decreased on the 30th day for all composites. Polymerization time did not affect the monomer release from the composites, but the type of the monomers and concentration of the filler used in the composites affected the amount of released monomers. The use of TEGDMA (co)monomer reduced the monomer release.

Fruit production in kiwifruit (Actinidia deliciosa) using preserved pollen

2004 ◽  
Vol 55 (5) ◽  
pp. 565 ◽  
Author(s):  
I. Abreu ◽  
M. Oliveira
Keyword(s):  
Storage Period ◽  
Fruit Production ◽  
Actinidia Deliciosa ◽  
High Viability ◽  
Hand Pollination ◽  
Male Flowers ◽  
And Storage ◽  
Direct Immersion

The influence of temperature and storage period on pollen quality of Actinidia deliciosa (A. Chev.) C. F. Liang & A. R. Ferguson was studied. Pollen collected from male flowers was stored under different conditions (20°C, 65% RH; –20°C, 51% RH; –80°C, 55% RH and –196°C by direct immersion in liquid nitrogen). During the preservation period, viability and in vitro germination percentages were evaluated at regular periods. The results show that –20°C was the best temperature at which to preserve pollen of A. deliciosa because it retains high viability and germination. At 20°C, germination was totally lost within 8 weeks, and at –80°C or –196°C germination drastically dropped over the same period of preservation. In the 2002 blossoming season, the pollen preserved at –20°C was used for hand pollination in order to estimate its seed set capacity. Thirty days after fruit-set, all fruits were long and well shaped, indicating a successful pollination. Our results indicate a simple and reliable method to preserve pollen of A. deliciosa that can be easily used by farmers.

The in vitro germination and storage characteristics of Keteleeria fortunei var. cyclolepis pollen provide a reference for cross breeding

PROTOPLASMA ◽  
2020 ◽  
Vol 257 (4) ◽  
pp. 1221-1230
Author(s):  
Xiongsheng Liu ◽  
Yufei Xiao ◽  
Yong Wang ◽  
Fengfan Chen ◽  
Ronglin Huang ◽  
...  
Keyword(s):  
In Vitro Germination ◽  
Cross Breeding ◽  
And Storage ◽  
Storage Characteristics
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