Quantitative Spin-trapping ESR Investigation of Alkoxyl Radical Derived from AAPH: Development of a Flow-injection Spin-trapping ESR System for the Oxygen Radical Absorbance Capacity Assay

2015 ◽  
Vol 46 (9) ◽  
pp. 1013-1022 ◽  
Author(s):  
Akira Nakajima ◽  
Tomoko Yamaguchi ◽  
Tomoyuki Yamashita ◽  
Kiyoshi Kawai ◽  
Yusuke Miyake ◽  
...  
Keyword(s):  
Flow Injection ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Alkoxyl Radical

scholarly journals Radical Scavenging Ability by ESR Spin Trapping and Oxygen Radical Absorbance Capacity of Hot Water Extracts from Mushrooms

2013 ◽  
Vol 60 (4) ◽  
pp. 173-178 ◽  
Author(s):  
Tomomi Kanno ◽  
Shouei Kawamura ◽  
Etsuko Harada ◽  
Hiromi Kameya ◽  
Mitsuko Ukai ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Hot Water ◽  
Oxygen Radical Absorbance Capacity ◽  
Water Extracts ◽  
Esr Spin Trapping ◽  
Radical Scavenging Ability

Is the Oxygen Radical Absorbance Capacity (ORAC) Method a Peroxyl-radical Scavenging Assay?

2010 ◽  
Vol 224 (06) ◽  
pp. 921-928 ◽  
Author(s):  
Yoshimi Sueishi ◽  
Daisuke Yoshioka ◽  
Shigeru Oowada ◽  
Nobuyuki Endoh ◽  
Shunji Kohri ◽  
...  
Keyword(s):  
Peroxyl Radical ◽  
Radical Scavenging Activity ◽  
Aqueous Media ◽  
Radical Scavenging ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Water Soluble ◽  
Oxygen Radical Absorbance Capacity ◽  
Peroxyl Radicals ◽  
Radical Initiator

AbstractThe oxygen radical absorbance capacity (ORAC) method employs a water soluble azo-radical initiator, AAPH (2,2’-azobis(2-amidinopropane) dihydrochloride) as a free radical generator, by which the fluorescent probe fluorescein is damaged to result in the loss of fluorescence. Antioxidants can protect the probe from the damage and the degree of protection is quantified. Because AAPH has been used as a lipid-peroxidation reagent, “oxygen radical” in ORAC is generally accepted as peroxyl radicals; however, in the present spin trapping experiments using a newly developed spin trap, CYPMPO, there was no indication of peroxyl-radical formation in AAPH decomposition in aqueous media. These spin trapping studies demonstrated that alkoxyl (RO·) radical adduct was the sole product of AAPH decomposition. In contrast, alkyl-peroxyl (ROO·) radical was spin-trapped during the decomposition of a lipid soluble azo-radical initiator AIBN (azobis(isobutyronitrile)) in non-aqueous media. We speculate that alkyl-peroxyl radicals are short-lived in water, rapidly converted into alkoxyl radicals. Although the possibility that ORAC method monitors peroxyl-radical scavenging activity cannot be completely eliminated, spin trapping evidence strongly indicates that ORAC method is a scavenging capacity assay for alkoxyl radicals.

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

scholarly journals Determination of the Antioxidative Activities of Herbs Harvested in Japan by Oxygen Radical Absorbance Capacity Methods

2016 ◽  
Vol 22 (2) ◽  
pp. 301-305
Author(s):  
Manabu Wakagi ◽  
Yuuki Taguchi ◽  
Jun Watanabe ◽  
Tasuku Ogita ◽  
Masao Goto ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

Role of alkoxyl radicals on the fluorescein-based ORAC (Oxygen Radical Absorbance Capacity) assay

2014 ◽  
Vol 75 ◽  
pp. S38 ◽  
Author(s):  
E Dorta ◽  
E Atala ◽  
A Aspee ◽  
H Speisky ◽  
E Lissi ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

scholarly journals Chemical Constituents and Antioxidant Activity of Ficus callosa

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Phan Van Kiem ◽  
Nguyen Xuan Cuong ◽  
Nguyen Xuan Nhiem ◽  
Dan Thi Thuy Hang ◽  
Nguyen Hoai Nam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Chemical Evidence

One new megastigmane glycoside, ficalloside (1), and eleven known compounds, were isolated from methanol extract of Ficus callosa leaves by repeated column chromatography. Their structures were established on the basis of spectral and chemical evidence. The antioxidant activities of these compounds were measured using the oxygen radical absorbance capacity (ORAC) assay. Compound 8 exhibited potent antioxidant activity of 10.6 μM trolox equivalents at the concentration of 2 μM. At this concentration, compounds 4-7 and 9-12 showed significant antioxidant activity with ranging of 2.1~6.1 μM trolox equivalents.

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