scholarly journals Radical Scavenging Ability by ESR Spin Trapping and Oxygen Radical Absorbance Capacity of Hot Water Extracts from Mushrooms

2013 ◽  
Vol 60 (4) ◽  
pp. 173-178 ◽  
Author(s):  
Tomomi Kanno ◽  
Shouei Kawamura ◽  
Etsuko Harada ◽  
Hiromi Kameya ◽  
Mitsuko Ukai ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Hot Water ◽  
Oxygen Radical Absorbance Capacity ◽  
Water Extracts ◽  
Esr Spin Trapping ◽  
Radical Scavenging Ability

Development of a HPLC-ESR Spin-trapping System for Post-column On-line Detection of Superoxide Radical Scavenging Ability of Column Eluates

2009 ◽  
Vol 38 (7) ◽  
pp. 662-663 ◽  
Author(s):  
Yasuhiro Sakurai ◽  
Kiyoshi Kawai ◽  
Rushiru Komatsu-Watanabe ◽  
Chie Morimoto ◽  
Shunji Sakamoto ◽  
...  
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging ◽  
Spin Trapping ◽  
Line Detection ◽  
Esr Spin Trapping ◽  
On Line ◽  
Radical Scavenging Ability

Peroxyl Radical Scavenging Ability of Fifty Kinds of Chinese Medicines Determined by an ESR Spin-Trapping Technique

2012 ◽  
Vol 549 ◽  
pp. 327-330
Author(s):  
Fu Hai Wang ◽  
Cheng Hua Huang
Keyword(s):  
Peroxyl Radical ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Spin Trapping ◽  
Chinese Medicines ◽  
Esr Spin Trapping ◽  
Spin Resonance ◽  
Radical Scavenging Ability ◽  
Thermolysis Reaction ◽  
Spin Trapping Technique

The peroxyl radical scavenging abilities of fifty Chinese herbal medicines were determined accurately by an electron spin resonance (ESR) spin-trapping technique using 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) as a spin-trapping reagent. The peroxyl radical was generated by the thermolysis reaction of 2,2’-Azobis(isobutyronitrile) (AIBN) in the presence of the spin-tap DMPO and with or without sample. As a result of these studies, a strong scavenging ability of peroxyl radical was found in Chinese medicines for diseases of anti-aging and blood circulation.

Is the Oxygen Radical Absorbance Capacity (ORAC) Method a Peroxyl-radical Scavenging Assay?

2010 ◽  
Vol 224 (06) ◽  
pp. 921-928 ◽  
Author(s):  
Yoshimi Sueishi ◽  
Daisuke Yoshioka ◽  
Shigeru Oowada ◽  
Nobuyuki Endoh ◽  
Shunji Kohri ◽  
...  
Keyword(s):  
Peroxyl Radical ◽  
Radical Scavenging Activity ◽  
Aqueous Media ◽  
Radical Scavenging ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Water Soluble ◽  
Oxygen Radical Absorbance Capacity ◽  
Peroxyl Radicals ◽  
Radical Initiator

AbstractThe oxygen radical absorbance capacity (ORAC) method employs a water soluble azo-radical initiator, AAPH (2,2’-azobis(2-amidinopropane) dihydrochloride) as a free radical generator, by which the fluorescent probe fluorescein is damaged to result in the loss of fluorescence. Antioxidants can protect the probe from the damage and the degree of protection is quantified. Because AAPH has been used as a lipid-peroxidation reagent, “oxygen radical” in ORAC is generally accepted as peroxyl radicals; however, in the present spin trapping experiments using a newly developed spin trap, CYPMPO, there was no indication of peroxyl-radical formation in AAPH decomposition in aqueous media. These spin trapping studies demonstrated that alkoxyl (RO·) radical adduct was the sole product of AAPH decomposition. In contrast, alkyl-peroxyl (ROO·) radical was spin-trapped during the decomposition of a lipid soluble azo-radical initiator AIBN (azobis(isobutyronitrile)) in non-aqueous media. We speculate that alkyl-peroxyl radicals are short-lived in water, rapidly converted into alkoxyl radicals. Although the possibility that ORAC method monitors peroxyl-radical scavenging activity cannot be completely eliminated, spin trapping evidence strongly indicates that ORAC method is a scavenging capacity assay for alkoxyl radicals.

Quantitative Spin-trapping ESR Investigation of Alkoxyl Radical Derived from AAPH: Development of a Flow-injection Spin-trapping ESR System for the Oxygen Radical Absorbance Capacity Assay

2015 ◽  
Vol 46 (9) ◽  
pp. 1013-1022 ◽  
Author(s):  
Akira Nakajima ◽  
Tomoko Yamaguchi ◽  
Tomoyuki Yamashita ◽  
Kiyoshi Kawai ◽  
Yusuke Miyake ◽  
...  
Keyword(s):  
Flow Injection ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Alkoxyl Radical

Synthesis of water-soluble cystine C60 derivative with catalyst and its active oxygen radical scavenging ability

2007 ◽  
Vol 18 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Zhen Hu ◽  
Wen Chao Guan ◽  
Xiang Ying Tang ◽  
Li Zhen Huang ◽  
Hong Xu
Keyword(s):  
Radical Scavenging ◽  
Oxygen Radical ◽  
Active Oxygen ◽  
Water Soluble ◽  
Radical Scavenging Ability

Active oxygen radical scavenging ability of water-soluble fullerenols

1997 ◽  
Vol 42 (9) ◽  
pp. 748-752 ◽  
Author(s):  
Dayong Sun ◽  
Yanshi Zhu ◽  
Ziyang Liu ◽  
Guizhen Liu ◽  
Xinghua Guo ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Oxygen Radical ◽  
Active Oxygen ◽  
Water Soluble ◽  
Radical Scavenging Ability

Seasonal variations of oxygen radical scavenging ability in rosemary leaf extract

2018 ◽  
Vol 245 ◽  
pp. 270-274 ◽  
Author(s):  
Yoshimi Sueishi ◽  
Misato Sue ◽  
Hiroaki Masamoto
Keyword(s):  
Seasonal Variations ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Oxygen Radical ◽  
Radical Scavenging Ability
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