Binding of HIV-1 TAR mRNA to a peptide nucleic acid oligomer and its conjugates with metal-ion-binding multidentate ligands

2008 ◽  
Vol 14 (2) ◽  
pp. 287-300 ◽  
Author(s):  
Matthew J. Belousoff ◽  
Gilles Gasser ◽  
Bim Graham ◽  
Yitzhak Tor ◽  
Leone Spiccia
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Metal Ion ◽  
Ion Binding ◽  
Multidentate Ligands ◽  
Metal Ion Binding ◽  
Hiv 1

scholarly journals Adenosine 5'-triphosphate (ATP4-): Aspects of the coordination chemistry of a multitalented biological substrate

2004 ◽  
Vol 76 (2) ◽  
pp. 375-388 ◽  
Author(s):  
H. Sigel
Keyword(s):  
Nucleic Acid ◽  
Metal Ions ◽  
Metal Ion ◽  
Hydrophobic Interactions ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Chelate Formation ◽  
Stability Of Complexes ◽  
Biological Substrate ◽  
The Stability

Firstly, the self-stacking properties of ATP4- and the effects of metal ions and protons on these properties are described. Some examples involving macrochelate formation between phosphate-coordinated metal ions (M2+) and N7 of the adenine residue in MATP2- are discussed, and this is followed by considerations on mixed ligand complexes consisting of ATP4-, M2+, and amino acid anions with side chains that allow either aromatic-ring stacking or hydrophobic interactions with the adenine moiety; this gives rise to selectivity. Next, the properties of diphosphorylated 9-[2-(phosphonomethoxy)ethyl]adenine (PMEA2-; Adefovir), i.e., of PMEApp4-, are compared with those of (2'-deoxy)ATP4- with regard to their metal ion-binding qualities, and in this way it can be explained why PMEApp2- is initially an excellent substrate for nucleic acid polymerases. Of course, after incorporation of the PMEA residue into the growing nucleic acid chain, this is terminated and this is how PMEA exerts its antiviral properties [its bis(pivaloyloxymethyl)ester, Adefovir dipivoxil, was recently approved for use in hepatitis B therapy]. Finally, the change in free energy connected with (macro)chelate formation or intramolecular stacking interactions and the effect of a reduced dielectric constant of the solvent on the stability of complexes and their structures in solution is considered.

Photophysical Characterisation, Metal Ion Binding and Enantiomeric Recognition of Chiral Ligands Containing Phenazine Fluorophore

2004 ◽  
Vol 69 (4) ◽  
pp. 885-896 ◽  
Author(s):  
Luisa Stella Dolci ◽  
Péter Huszthy ◽  
Erika Samu ◽  
Marco Montalti ◽  
Luca Prodi ◽  
...  
Keyword(s):  
Metal Ion ◽  
Photophysical Properties ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Ammonium Ions ◽  
Enantiomerically Pure ◽  
Binding Process ◽  
High Affinity ◽  
Enantiomeric Recognition ◽  
Chiral Species

Enantiomerically pure dimethyl- and diisobutyl-substituted phenazino-18-crown-6 ligands bind metal and ammonium ions and also primary aralkylammonium perchlorates in acetonitrile with high affinity, causing pronounced changes in their luminescence properties. In addition, they show enantioselectivity towards chiral primary aralkylammonium perchlorates. The possibility to monitor the binding process by photoluminescence spectroscopy can gain ground for the design of very efficient enantioselective chemosensors for chiral species. The observed changes in the photophysical properties are also an important tool for understanding the interactions present in the adduct.

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