scholarly journals Effects of casein phosphopeptide-amorphous calcium phosphate crème on nicotine-induced Streptococcus mutans biofilm in vitro

2020 ◽  
Vol 24 (10) ◽  
pp. 3513-3518
Author(s):  
Naser B. Alawadhi ◽  
Frank Lippert ◽  
Richard L. Gregory
Keyword(s):  
Calcium Phosphate ◽  
Streptococcus Mutans ◽  
Amorphous Calcium Phosphate ◽  
Casein Phosphopeptide

scholarly journals THE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT’S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION

2017 ◽  
Vol 10 (17) ◽  
pp. 174
Author(s):  
Sri Angky Soekanto ◽  
Gadiacanaparimita Ghrena Duhita ◽  
Endang Winiati Bachtiar ◽  
Muhamad Sahlan
Keyword(s):  
Calcium Phosphate ◽  
Streptococcus Mutans ◽  
Calcium Ions ◽  
Amorphous Calcium Phosphate ◽  
Chewing Gum ◽  
Phosphate Ion ◽  
Phosphate Ions ◽  
Casein Phosphopeptide ◽  
Mass Formation

 Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum.Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader.Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p<0.05) and phosphate ions (p>0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05).Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.

scholarly journals Comparison of the Efficacy of Different Fluoride Varnishes on Dentin Remineralization During a Critical pH Exposure Using Quantitative X-Ray Microtomography

2018 ◽  
Vol 43 (6) ◽  
pp. E308-E316
Author(s):  
A Sleibi ◽  
A Tappuni ◽  
D Mills ◽  
GR Davis ◽  
A Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Mineral Content ◽  
Test Group ◽  
Control Group ◽  
Mineral Concentration ◽  
Voxel Size ◽  
X Ray ◽  
Casein Phosphopeptide

SUMMARY Objectives: The objective of this in vitro study was to quantify the amount of mineral change in demineralized dentin at pH 5.5 after the application of dental varnishes containing fluoride with casein phosphopeptide–amorphous calcium phosphate, fluoride and bioglass, or fluoride alone. Methods and Materials: A total of 12 extracted human sound mandibular premolar root samples were coated with an acid-resistant varnish, leaving a 2 × 3 mm window at the outer root surface. These root specimens were then randomly divided into four groups and separately subjected to the demineralizing cycle at a pH of 4.8 for five days to create artificial caries-like lesions in dentin. Subsequently, each sample was imaged using quantitative x-ray microtomography (XMT) at a 15-μm voxel size. Each test group then received one of the following treatments: dental varnish containing casein phosphopeptide–amorphous calcium phosphate and fluoride (CPP-ACP, MI varnish, GC Europe), bioglass and fluoride (BGA, Experimental, Dentsply Sirona), or fluoride alone (NUPRO, Dentsply Sirona), as well as a control group, which received no treatment. These samples were kept in deionized water for 12 hours. The thin layer of varnish was then removed. All samples including the nonvarnish group were subjected to the second demineralizing cycle at pH 5.5 for five days. The final XMT imaging was then carried out following the second demineralizing cycle. XMT scan was also carried out to varnish samples at 25 μm voxel size. The change in mineral concentration in the demineralized teeth was assessed using both qualitative and quantitative image analysis. Results: There was an increase in radiopacity in the subtracted images of all varnish groups; a significant increase in mineral content, 12% for the CPP-ACP and fluoride (p≤0.05 and p≤0.001), 25% BGA (p≤0.001), and 104% fluoride alone varnish (p≤0.001). There was an increase in the size of radiolucency in the lesion area with a significant decrease in mineral content in the nonvarnish group, 10% (p≤0.05 and p≤0.001). Conclusions: There was encouraging evidence of a remineralization effect following the application of dental varnish on dentin and also an observed resistance to demineralization during the acidic challenge in all cases. However, a dental varnish containing fluoride alone appeared to have a much greater effect on dentin remineralization when compared with CPP-ACP with fluoride and bioglass with fluoride.

Effect of CPP-ACP Application on Flexural Strength of Bleached Enamel and Dentin Complex

2011 ◽  
Vol 36 (4) ◽  
pp. 372-379 ◽  
Author(s):  
M Khoroushi ◽  
H Mazaheri ◽  
AE Manoochehri
Keyword(s):  
Calcium Phosphate ◽  
Flexural Strength ◽  
Amorphous Calcium Phosphate ◽  
Clinical Relevance ◽  
In Vitro Simulation ◽  
Casein Phosphopeptide ◽  
Bovine Enamel ◽  
At Home

Clinical Relevance In the absence of saliva, flexural strength of the bovine enamel-dentin complex decreases after in vitro simulation of both at-home and in-office bleaching procedures. Application of Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) subsequent to both bleaching regimens could compensate for this decrease.

scholarly journals Effect of fluoridated casein phospopeptide-amorphous-calcium phosphate complex, chlorhexidine fluoride mouthwash on shear bond strength of orthodontic brackets: A comparative in vitro study

2016 ◽  
Vol 6 ◽  
pp. 12-17
Author(s):  
Abdul Shahariyar C. A ◽  
Anurag D. Mahale ◽  
Kishore Kumar ◽  
Rohan Rai
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Testing Machine ◽  
In Vitro Study ◽  
Conventional Technique ◽  
Orthodontic Brackets ◽  
Healthy Human ◽  
Bond Strengths ◽  
Casein Phosphopeptide

Objective The aim of the current study was to determine the effects of casein phosphopeptide amorphous calcium-phosphate (CPP-ACP) complex, chlorhexidine fluoride mouthwash on shear bond strengths (SBSs) of orthodontic brackets. Materials and Methods About sixty extracted healthy human premolar teeth with intact buccal enamel were divided into two equal groups to which brackets were bonded using self-etching primers (SEPs) and conventional means respectively. These were further equally divided into three subgroups - (1) control (2) CPP-ACP (3) chlorhexidine fluoride mouthwash. The SBSs were then measured using a universal testing machine. Results SBS of the conventional group was significantly higher than the self-etching group. The intragroup differences were statistically insignificant. Conclusion CPP-ACP, chlorhexidine fluoride mouthwash did not adversely affect SBS of orthodontic brackets irrespective of the method of conditioning. Brackets bonded with conventional technique showed greater bond strengths as compared to those bonded with SEP.

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