A novel lineage of sulfate-reducing microorganisms: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense , gen. nov., sp. nov., a new thermophilic isolate from a hot spring

Extremophiles ◽  
2003 ◽  
Vol 7 (4) ◽  
pp. 283-290 ◽  
Author(s):  
Koji Mori ◽  
Hongik Kim ◽  
Takeshi Kakegawa ◽  
Satoshi Hanada
Keyword(s):  
Hot Spring ◽  
Sulfate Reducing

scholarly journals Desulfosoma caldarium gen. nov., sp. nov., a thermophilic sulfate-reducing bacterium from a terrestrial hot spring

2011 ◽  
Vol 61 (4) ◽  
pp. 732-736 ◽  
Author(s):  
Sandra Baena ◽  
Natalia Perdomo ◽  
Catalina Carvajal ◽  
Carolina Díaz ◽  
Bharat K. C. Patel
Keyword(s):  
Gene Sequence ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Terminal Electron Acceptor ◽  
Sulfate Reducing ◽  
Colombian Andes ◽  
Gene Sequence Analysis ◽  
Lactate Degradation ◽  
Terrestrial Hot Spring

A thermophilic, sulfate-reducing bacterium, designated strain USBA-053T, was isolated from a terrestrial hot spring located at a height of 2500 m in the Colombian Andes (5° 45′ 33.29″ N 73° 6′ 49.89″ W), Colombia. Cells of strain USBA-053T were oval- to rod-shaped, Gram-negative and motile by means of a single polar flagellum. The strain grew autotrophically with H2 as the electron donor and heterotrophically on formate, propionate, butyrate, valerate, isovalerate, lactate, pyruvate, ethanol, glycerol, serine and hexadecanoic acid in the presence of sulfate as the terminal electron acceptor. The main end products from lactate degradation, in the presence of sulfate, were acetate, CO2 and H2S. Strain USBA-053T fermented pyruvate in the absence of sulfate and grew optimally at 57 °C (growth temperature ranged from 50 °C to 62 °C) and pH 6.8 (growth pH ranged from 5.7 to 7.7). The novel strain was slightly halophilic and grew in NaCl concentrations ranging from 5 to 30 g l−1, with an optimum at 25 g l−1 NaCl. Sulfate, thiosulfate and sulfite were used as electron acceptors, but not elemental sulfur, nitrate or nitrite. The G+C content of the genomic DNA was 56±1 mol%. 16S rRNA gene sequence analysis indicated that strain USBA-053T was a member of the class Deltaproteobacteria, with Desulfacinum hydrothermale MT-96T as the closest relative (93 % gene sequence similarity). On the basis of physiological characteristics and phylogenetic analysis, it is suggested that strain USBA-053T represents a new genus and novel species for which the name Desulfosoma caldarium gen. nov., sp. nov. is proposed. The type strain of the type species is USBA-053T ( = KCTC 5670T = DSM 22027T).

scholarly journals High Rates of Sulfate Reduction in a Low-Sulfate Hot Spring Microbial Mat Are Driven by a Low Level of Diversity of Sulfate-Respiring Microorganisms

2007 ◽  
Vol 73 (16) ◽  
pp. 5218-5226 ◽  
Author(s):  
Jesse G. Dillon ◽  
Susan Fishbain ◽  
Scott R. Miller ◽  
Brad M. Bebout ◽  
Kirsten S. Habicht ◽  
...  
Keyword(s):  
National Park ◽  
Hot Spring ◽  
Sulfur Cycle ◽  
Microbial Mat ◽  
Carbon Limitation ◽  
Oxygen Production ◽  
Sulfate Reducing ◽  
Highly Active ◽  
Photosynthetic Oxygen ◽  
Reduced Activity

ABSTRACT The importance of sulfate respiration in the microbial mat found in the low-sulfate thermal outflow of Mushroom Spring in Yellowstone National Park was evaluated using a combination of molecular, microelectrode, and radiotracer studies. Despite very low sulfate concentrations, this mat community was shown to sustain a highly active sulfur cycle. The highest rates of sulfate respiration were measured close to the surface of the mat late in the day when photosynthetic oxygen production ceased and were associated with a Thermodesulfovibrio-like population. Reduced activity at greater depths was correlated with novel populations of sulfate-reducing microorganisms, unrelated to characterized species, and most likely due to both sulfate and carbon limitation.

scholarly journals Desulfotomaculum hydrothermale sp. nov., a thermophilic sulfate-reducing bacterium isolated from a terrestrial Tunisian hot spring

2008 ◽  
Vol 58 (11) ◽  
pp. 2529-2535 ◽  
Author(s):  
O. Haouari ◽  
M.-L. Fardeau ◽  
J.-L. Cayol ◽  
C. Casiot ◽  
F. Elbaz-Poulichet ◽  
...  
Keyword(s):  
Hot Spring ◽  
Sulfate Reducing

scholarly journals Thioarsenate Formation Coupled with Anaerobic Arsenite Oxidation by a Sulfate-Reducing Bacterium Isolated from a Hot Spring

2017 ◽  
Vol 8 ◽  
Author(s):  
Geng Wu ◽  
Liuqin Huang ◽  
Hongchen Jiang ◽  
Yue’e Peng ◽  
Wei Guo ◽  
...  
Keyword(s):  
Hot Spring ◽  
Arsenite Oxidation ◽  
Sulfate Reducing

Enrichment and isolation of acid-tolerant sulfate-reducing microorganisms in the anoxic, acidic hot spring sediments from Copahue volcano, Argentina

2019 ◽  
Vol 95 (12) ◽  
Author(s):  
Graciana Willis ◽  
Ivan Nancucheo ◽  
Sabrina Hedrich ◽  
Alejandra Giaveno ◽  
Edgardo Donati ◽  
...  
Keyword(s):  
Ferric Iron ◽  
Hot Spring ◽  
Low Ph ◽  
Acidophilic Microorganisms ◽  
Sulfate Reducing ◽  
Ph Values ◽  
Organic Electron ◽  
Acid Tolerant ◽  
Biotechnological Processes ◽  
Iron Precipitates

ABSTRACT The geothermal Copahue-Caviahue (GCC) system (Argentina) is an extreme acidic environment, dominated by the activity of Copahue volcano. Environments characterised by low pH values, such as volcanic areas, are of particular interest for the search of acidophilic microorganisms with application in biotechnological processes. In this work, sulfate-reducing microorganisms were investigated in geothermal acidic, anaerobic zones from GCC system. Sediment samples from Agua del Limón (AL1), Las Máquinas (LMa2), Las Maquinitas (LMi) and Baño 9 (B9–2, B9–3) were found to be acidic (pH values 2.1–3.0) to moderate acidic (5.1–5.2), containing small total organic carbon values, and ferric iron precipitates. The organic electron donor added to the enrichment was completely oxidised to CO2. Bacteria related to ‘Desulfobacillus acidavidus’ strain CL4 were found to be dominant (67–83% of the total number of clones) in the enrichment cultures, and their presence was confirmed by their isolation on overlay plates. Other bacteria were also detected with lower abundance (6–20% of the total number of clones), with representatives of the genera Acidithiobacillus, Sulfobacillus, Alicyclobacillus and Athalassotoga/Mesoaciditoga. These enrichment and isolates found at low pH confirm the presence of anaerobic activities in the acidic sediments from the geothermal Copahue-Caviahue system.

Microbial Diversity in Acidic Anaerobic Sediments at the Geothermal Caviahue-Copahue System, Argentina

2013 ◽  
Vol 825 ◽  
pp. 7-10 ◽  
Author(s):  
Graciana Willis ◽  
Sabrina Hedrich ◽  
Ivan Nancucheo ◽  
D. Barrie Johnson ◽  
Edgardo R. Donati
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Hot Spring ◽  
Rrna Gene ◽  
Sulfate Reducer ◽  
Sulfate Reducing ◽  
Culture Independent ◽  
Cultivation Techniques ◽  
Reducing Bacteria ◽  
The 16S Rrna Gene

In this work we have examined the bacterial diversity from the hot spring sediment Agua del Limón (AL1) present at the geothermal Caviahue-Copahue system using a combination of molecular and cultivation techniques, with particular emphasis on indigenous anaerobic prokaryotes. Microorganisms involved in the iron (Acidithiobacillus ferrooxidansandLeptospirillumspp.) and sulphur (Acidithiobacillusspp., Thermotogales-like bacteria,Thiomonassp., andDesulfurellasp.) cycles were identified in the clone library. Although no obvious sulfate-reducing bacteria were detected by culture-independent techniques, several isolates related to the mesophilic, spore-forming sulfate-reducer"Desulfobacillus acidavidus"strain CL4 were isolated at 30°C and 40°C. The 16S rRNA gene of another isolate showed 94% similarity toDesulfotomaculum thermobenzoicum. Sulfate-reducing enrichment cultures of the Copahue samples were also dominated by"Dsb. acidavidus"CL4.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI TERMOFILIK PEREDUKSI SULFAT DI AIR PANAS SARONGSONG KOTA TOMOHON

Jurnal Biota ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 36-40
Author(s):  
Dany Christian Posumah ◽  
Dewianti A Rondonuwu
Keyword(s):  
Color Change ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Sulfate Reducing Bacteria ◽  
Dilution Method ◽  
Matching Method ◽  
Sulfate Reducing ◽  
Profile Matching ◽  
Hot Bath ◽  
Reducing Bacteria

ABSTRAK.Bakteri Pereduksi Sulfat (BPS) berperan dalam biodegradasi lahan tercemar. Identifikasi berdasarkan karakter fenotipik perlu dilakukan untuk mengetahui jenis bakteri ini. Penelitian ini bertujuan untuk mendapatkan beberapa isolat bakteri pereduksi sulfat di lokasi pemandian air panas Saronsong kota Tomohon. Identifikasi  dilakukan dengan menggunakan metode profile matching. Bakteri termofilik pereduksi sulfat diisolasi dari lokasi pemandian air panas Sarongsong kota Tomohon menggunakan media cair postgate b yang telah dimodifikasi dan selanjutnya dilakukan pemurnian menggunakan metode pengenceran. Karakter fenotipik isolat bakteri termofilik pereduksi sulfat yang diuji meliputi morfologi koloni, morfologi sel dan karakter biokimia. Hasil isolasi pada kawasan air panas Sarongsong diperoleh 4 isolat bakteri termofilik pereduksi sulfat yang dilihat dari perubahan warna media selektif. Berdasarkan identifikasi dengan menggunakan metode profile matching menunjukkan bahwa keempat isolat tersebut diduga termasuk dalam anggota genus Desulfotomaculum (isolat AK4D3 dan L4D1), genus  Desulfomicrobium (isolat AJ4D5) dan genus Desulfobulbus (isolat L5D2).Kata Kunci:, fenotipik, identifikasi, isolasi, karakterisasi ABSTRACT. Sulfate Reducing bacteria play a role in biodegradation  of land contaminated.  Identification based on phenotype character needs to be done to know this type of bacteria. The objective of this research is to get some isolate of sulfate reducing bacteria in clear water, cloudy water and mud in Tomohon hot spring water location. Identification is done by using profile matching method. The sulfate reducing thermophilic bacteria was isolated from the Tomohon Sarongsong hot spring location using the modified postgate b liquid medium and then purified by dilution method. Phenotypic characteristics of sulfate reducing thermophilic bacteria isolates tested included colony morphology, cell morphology and biochemical character. The result of isolation at Sarongsong hot bath obtained 4 isolates of sulfate reducing thermophilic bacteria seen from selective media color change. Based on the identification using the profile matching method showed that the four isolates were suspected to be included in the members of the genus Desulfotomaculum (isolates of AK4D3 and L4D1), genus Desulfomicrobium (isolate AJ4D5) and genus Desulfobulbus (isolate L5D2).  Keywords:,  characteristics,  Identification, Isolation, Phenotypic

scholarly journals Desulfothermobacter acidiphilus gen. nov., sp. nov., a thermoacidophilic sulfate-reducing bacterium isolated from a terrestrial hot spring

2018 ◽  
Vol 68 (3) ◽  
pp. 871-875 ◽  
Author(s):  
E. N. Frolov ◽  
K. S. Zayulina ◽  
D. S. Kopitsyn ◽  
I. V. Kublanov ◽  
E. A. Bonch-Osmolovskaya ◽  
...  
Keyword(s):  
Hot Spring ◽  
Sulfate Reducing ◽  
Terrestrial Hot Spring

scholarly journals Impact of the species compartment definition on quantitative modeling of microbial communities

2015 ◽  
Author(s):  
Marko Budinich ◽  
Jeremie Bourdon ◽  
Damien Eveillard
Keyword(s):  
Microbial Community ◽  
Hot Spring ◽  
Optimal Solution ◽  
Solution Space ◽  
Methanococcus Maripaludis ◽  
Sulfate Reducing ◽  
Single Compartment ◽  
Quantitative Changes ◽  
Community Modeling ◽  
Genome Scale

Recent advances in genome-scale metabolic network reconstruction paved the way to the use of quantitative modelings such as FBA. However, despite the great interest of these techniques to tackle quantitative features, microbial community modeling remains unclear. Whereas studies represent a microbial community with several compartments for each microbial strains and their common pool, others advocate for the use of a single compartment that combines all reactions. Here we show that both modelings lead to different optimal quantitative solutions. This study illustrates this difference by the use of the flux module technique, that describes, in a compact way, the optimal solution space as computed by FBA-like techniques. For application, this paper computes the flux modules of a hot spring microbial community (represented by Synechococcus spp., Chloroflexus and Roseiflexus spp.) and a microbial methagenic system (Desulvovibrio vulgaris and Methanococcus maripaludis) sulfate reducing bacteria), while emphasizing the quantitative changes that occurs when one assumes either the consortium as a “single compartment” or a multiple compartment.

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