Anti-cancer activity of the ethylacetate fraction from Orostachys japonicus for modulation of the signaling pathway in HepG2 human hepatoma cells

2013 ◽  
Vol 23 (1) ◽  
pp. 269-275 ◽  
Author(s):  
Gyeong-Seon Lee ◽  
Hyeong-Seon Lee ◽  
Seon-Hee Kim ◽  
Dong-Hee Suk ◽  
Deok-Seon Ryu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Anti Cancer ◽  
Cancer Activity

Extraction of ursolic acid from Ocimum sanctum and synthesis of its novel derivatives: effects on extracellular homocysteine, dihydrofolate reductase activity and proliferation of HepG2 human hepatoma cells

Pteridines ◽  
2013 ◽  
Vol 24 (3) ◽  
pp. 191-199 ◽  
Author(s):  
Apsara Batra ◽  
V. Girija Sastry
Keyword(s):  
Cancer Cells ◽  
Ursolic Acid ◽  
Dihydrofolate Reductase ◽  
Carbon Metabolism ◽  
Hepatoma Cells ◽  
Ocimum Sanctum ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Anti Cancer ◽  
One Carbon Metabolism

AbstractThe objective of the present study was to extract ursolic acid (UA) from Ocimum sanctum, to synthesize its bioactive derivatives, evaluate the anti-cancer effect of its derivatives and to establish the possible mechanism of action. In the present report, we extracted UA from whole plant of O. sanctum, synthesized its novel derivatives and investigated their effect on homocysteine metabolism and dihydrofolate reductase (DHFR) activity of HepG2 cells. UA and its derivatives UA-1, UA-2 and UA-3 down-regulated DHFR activity and increased extracellular homocysteine. UA-2 showed significant anti-proliferation activity in cancer cells. Cancer cells have increased the requirement of pyrimidine base thymidylate due to rapid cell division. Thymidylate biosynthesis depends on sufficient pools of folate dependent enzymes like DHFR. In the present study, we examined the UA and its derivatives mediated perturbation of DHFR activity and extracellular homocysteine in HepG2 human hepatoma cells. After incubation with UA-2, a potent inhibition of DHFR activity was observed. Our results showed that derivatization of UA might adversely affect DHFR activity. Measurement of extracellular homocysteine indicated impaired one-carbon metabolism in cells treated with UA derivatives. In conclusion, our data suggest an anti-cancer role of UA and its derivatives via inhibition of one-carbon metabolism.

scholarly journals Sirtuin 3 enhanced drug sensitivity of human hepatoma cells through glutathione S-transferase pi 1/JNK signaling pathway

Oncotarget ◽  
2016 ◽  
Vol 7 (31) ◽  
pp. 50117-50130 ◽  
Author(s):  
Na-Na Tao ◽  
Hong-Zhong Zhou ◽  
Hua Tang ◽  
Xue-Fei Cai ◽  
Wen-Lu Zhang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Drug Sensitivity ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Glutathione S Transferase ◽  
Sirtuin 3 ◽  
Human Hepatoma Cells ◽  
Jnk Signaling ◽  
Jnk Signaling Pathway

Tanshinone IIA activates calcium-dependent apoptosis signaling pathway in human hepatoma cells

2011 ◽  
Vol 66 (1) ◽  
pp. 192-201 ◽  
Author(s):  
Zhi-Kai Dai ◽  
Jiang-Ke Qin ◽  
Jiao-E Huang ◽  
Yong Luo ◽  
Qing Xu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Hepatoma Cells ◽  
Tanshinone Iia ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Calcium Dependent
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