Evaluation of Six Commercial Tests and the Germ-Tube Test for Presumptive Identification of Candida albicans

1999 ◽  
Vol 18 (3) ◽  
pp. 188-191 ◽  
Author(s):  
P. Frey ◽  
J. E. Hoppe
Keyword(s):  
Candida Albicans ◽  
Germ Tube ◽  
Tube Test ◽  
Presumptive Identification

scholarly journals USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST

2014 ◽  
Vol 56 (6) ◽  
pp. 483-485 ◽  
Author(s):  
Antonella Souza Mattei ◽  
Sydney Hartz Alves ◽  
Cecília Bittencourt Severo ◽  
Luciana da Silva Guazzelli ◽  
Flávio de Mattos Oliveira ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Human Serum ◽  
Germ Tube ◽  
Clinical Samples ◽  
Candida Spp ◽  
Tube Test ◽  
Fresh Serum ◽  
Mueller Hinton ◽  
Presumptive Identification ◽  
Mueller Hinton Broth

Candida albicans is often isolated from clinical samples, thus its presumptive differentiation from other species of the same genus can be based on its ability to form the germ tube in human serum. Nevertheless, there are two other species that share this characteristic: C. dubliniensis and C. africana. The aim of this study was to compare four different substrates to perform the germ tube (GT) test. The Candida spp. isolates were identified using a manual system (135 C. albicans, 24 C. tropicalis and one C. dubliniensis). The germ tube test was performed with fresh, previously frozen serum and Mueller-Hinton (MH) broth and agar. GT was observed in 96% (130/136) of the isolates through the fresh serum technique, 94% (128/136) through previously frozen serum, 92% (125/136) in MH agar, and 90% (122/136) in MH broth. The sensitivity of each test was higher than 90%, with 100% specificity. Both the MH agar and broth were able to identify the true positives, and false positives were not found. However, some C. albicans isolates were not identified. MH agar and broth may be used in laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test.

Performance of BacticardTM Candida compared with the germ tube test for the presumptive identification of Candida albicans

Mycoses ◽  
2003 ◽  
Vol 46 (11-12) ◽  
pp. 467-470 ◽  
Author(s):  
A. J. Carrillo-Muñoz ◽  
G. Quindós ◽  
C. D. Cárdenes ◽  
R. Alonso-Vargas ◽  
S. Brió ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Germ Tube ◽  
Tube Test ◽  
Presumptive Identification

scholarly journals Chromogenic Tube Test for Presumptive Identification or Confirmation of Isolates as Candida albicans

1998 ◽  
Vol 36 (4) ◽  
pp. 1157-1159 ◽  
Author(s):  
John Merlino ◽  
Evanthia Tambosis ◽  
Duncan Veal
Keyword(s):  
Candida Albicans ◽  
Sensitivity And Specificity ◽  
False Positive ◽  
Germ Tube ◽  
Cost Effective ◽  
Tube Test ◽  
Cost Effective Method ◽  
Presumptive Identification ◽  
Positive Results ◽  
Better Than

This report describes a new, modified, simple, and cost-effective method for the use of CHROMagar Candida (CHROMagar Company, Paris, France) for the presumptive identification of isolates as Candida albicans after preliminary growth. Sixty randomly selected clinical isolates were evaluated, including 38 of C. albicans. With incubation at 37°C for 24 h, the sensitivity and specificity appeared to be excellent and the test performed better than the traditional germ tube test. However, at earlier times, C. tropicalis isolates gave false-positive results.

scholarly journals A study to evaluate etiology of denture stomatitis and to determine interrelationship with trauma and candida albicans count

2021 ◽  
Vol 7 (3) ◽  
pp. 151-156
Author(s):  
Deeksha Arya ◽  
Aditi Verma ◽  
Saumyendra Vikram Singh ◽  
Prashant Gupta ◽  
Anupama Pathak ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Germ Tube ◽  
Indian Population ◽  
Culture Media ◽  
Swab Sample ◽  
Significant Relation ◽  
Cross Sectional ◽  
Denture Stomatitis ◽  
Tube Test

Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to denture stomatitis. The purpose of this study is to identify the etiology of denture stomatitis and to establish the role of trauma and fungal infection in denture stomatitis.Subjects wearing previously fabricated removable partial or complete denture who were diagnosed with denture stomatitis were included. It is a cross sectional questionnaire-based study, for mycological study swab sample was smeared, Sabouraud Dextrose Agar (SDA) was used as culture media. Germ tube test was used to identify Candida albicans. To test significance between different variablesKruskal-Wallis test was used. Out of 195 subjects’ slight stomatitis was present in 52.80% patients, followed by moderate stomatitis in 32.31% and no stomatitis in 2.56% subjects. Candida was present in 25.64% patients. Candida significantly more common in moderate stomatitis than others.This study has suggested that there is a significant relation in denture stomatitis and Candida albicans in north Indian population. No significant role of trauma was noticed. Strong correlation has been found in Denture hygiene, cleaning habits and Denture Stomatitis.

Comparison of Albicans ID2® agar plate with the germ tube for presumptive identification of Candida albicans

2002 ◽  
Vol 42 (3) ◽  
pp. 181-185 ◽  
Author(s):  
C.D. Cárdenes ◽  
A.J. Carrillo ◽  
A. Arias ◽  
C. Rodrı́guez-Alvarez ◽  
A. Torres-Lana ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Germ Tube ◽  
Agar Plate ◽  
Presumptive Identification
Sign in / Sign up

Export Citation Format

Share Document