scholarly journals USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST

2014 ◽  
Vol 56 (6) ◽  
pp. 483-485 ◽  
Author(s):  
Antonella Souza Mattei ◽  
Sydney Hartz Alves ◽  
Cecília Bittencourt Severo ◽  
Luciana da Silva Guazzelli ◽  
Flávio de Mattos Oliveira ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Human Serum ◽  
Germ Tube ◽  
Clinical Samples ◽  
Candida Spp ◽  
Tube Test ◽  
Fresh Serum ◽  
Mueller Hinton ◽  
Presumptive Identification ◽  
Mueller Hinton Broth

Candida albicans is often isolated from clinical samples, thus its presumptive differentiation from other species of the same genus can be based on its ability to form the germ tube in human serum. Nevertheless, there are two other species that share this characteristic: C. dubliniensis and C. africana. The aim of this study was to compare four different substrates to perform the germ tube (GT) test. The Candida spp. isolates were identified using a manual system (135 C. albicans, 24 C. tropicalis and one C. dubliniensis). The germ tube test was performed with fresh, previously frozen serum and Mueller-Hinton (MH) broth and agar. GT was observed in 96% (130/136) of the isolates through the fresh serum technique, 94% (128/136) through previously frozen serum, 92% (125/136) in MH agar, and 90% (122/136) in MH broth. The sensitivity of each test was higher than 90%, with 100% specificity. Both the MH agar and broth were able to identify the true positives, and false positives were not found. However, some C. albicans isolates were not identified. MH agar and broth may be used in laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test.

Performance of BacticardTM Candida compared with the germ tube test for the presumptive identification of Candida albicans

Mycoses ◽  
2003 ◽  
Vol 46 (11-12) ◽  
pp. 467-470 ◽  
Author(s):  
A. J. Carrillo-Muñoz ◽  
G. Quindós ◽  
C. D. Cárdenes ◽  
R. Alonso-Vargas ◽  
S. Brió ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Germ Tube ◽  
Tube Test ◽  
Presumptive Identification

scholarly journals Chromogenic Tube Test for Presumptive Identification or Confirmation of Isolates as Candida albicans

1998 ◽  
Vol 36 (4) ◽  
pp. 1157-1159 ◽  
Author(s):  
John Merlino ◽  
Evanthia Tambosis ◽  
Duncan Veal
Keyword(s):  
Candida Albicans ◽  
Sensitivity And Specificity ◽  
False Positive ◽  
Germ Tube ◽  
Cost Effective ◽  
Tube Test ◽  
Cost Effective Method ◽  
Presumptive Identification ◽  
Positive Results ◽  
Better Than

This report describes a new, modified, simple, and cost-effective method for the use of CHROMagar Candida (CHROMagar Company, Paris, France) for the presumptive identification of isolates as Candida albicans after preliminary growth. Sixty randomly selected clinical isolates were evaluated, including 38 of C. albicans. With incubation at 37°C for 24 h, the sensitivity and specificity appeared to be excellent and the test performed better than the traditional germ tube test. However, at earlier times, C. tropicalis isolates gave false-positive results.

scholarly journals Influence of Human Serum on Pharmacodynamic Properties of an Investigational Glycopeptide, LY333328, and Comparator Agents against Staphylococcus aureus

1998 ◽  
Vol 42 (9) ◽  
pp. 2427-2430 ◽  
Author(s):  
George G. Zhanel ◽  
Iain D. C. Kirkpatrick ◽  
Daryl J. Hoban ◽  
Amin M. Kabani ◽  
James A. Karlowsky
Keyword(s):  
Staphylococcus Aureus ◽  
Human Serum ◽  
Mueller Hinton ◽  
Mueller Hinton Broth

ABSTRACT The MICs and MBCs of 15 antibiotics for two strains ofStaphylococcus aureus were determined in Mueller-Hinton broth (MHB) and 90% serum–10% MHB. Subsequent experiments established that highly protein-bound antibiotics (≥80%), such as LY333328, demonstrated higher MICs and MBCs, less killing over an 8-h interval, and shorter postantibiotic effects in 90% serum–10% MHB than in MHB alone. Albumin was demonstrated to be almost solely responsible for changes in the aforementioned pharmacodynamic parameters of LY333328.

Can serums be replaced by Mueller-Hinton agar in germ tube test?

2017 ◽  
Vol 20 (1) ◽  
pp. 61
Author(s):  
MA Atalay ◽  
AN Koc ◽  
OM Parkan ◽  
G Aydemir ◽  
F Elmali ◽  
...  
Keyword(s):  
Germ Tube ◽  
Tube Test ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

scholarly journals Experimental Germ Tube Induction in Candida albicans: An Evaluation of the Effect of Sodium Bicarbonate on Morphogenesis and Comparison with Pooled Human Serum

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Tapiwa Matare ◽  
Pasipanodya Nziramasanga ◽  
Lovemore Gwanzura ◽  
Valerie Robertson
Keyword(s):  
Candida Albicans ◽  
Human Serum ◽  
Germ Tube ◽  
Tube Formation ◽  
Joint Research ◽  
Significant Difference ◽  
Buffer Control ◽  
Tris Maleate ◽  
Microscope Slides ◽  
Germ Tubes

Objective. The potential of NaHCO3 versus human serum to induce germ tube formation in Candida albicans was investigated. Specimens. A total of 100 isolates were obtained from oral swabs of patients presenting with thrush. Approval for the study was granted by the Joint Research Ethics Committee (JREC/23/08). Method. Confirmed C. albicans isolates by routine methods were tested for germ tube induction using 5 different concentrations of Tris-maleate buffered NaHCO3 and Tris-maleate buffer control. Standard control strains included were C. albicans (ATCC 10231) and C. krusei (ATCC 6258). Microculture was done in 20 μL inoculums on microscope slides for 3 hours at 37°C. The rate of germ tube formation at 10-minute intervals was determined on 100 isolates using the optimum 20 mM Tris-maleate buffered NaHCO3 concentration. Parallel germ tube formation using human serum was done in test tubes. Results. The optimum concentration of NaHCO3 in Tris-maleate buffer for germ tube induction was 20 mM for 67% of isolates. Only 21% of isolates formed germ tubes in Tris-maleate buffer control. There was no significant difference in induction between human serum and Tris-maleate buffered NaHCO3. Conclusion. Tris-maleate buffered NaHCO3 induced germ tube formation in C. albicans isolates at rates similar to human serum.

scholarly journals COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF Candida SPECIES

2015 ◽  
Vol 57 (4) ◽  
pp. 281-287 ◽  
Author(s):  
Margarida Neves SOUZA ◽  
Stéfanie Otowicz ORTIZ ◽  
Marcelo Martins MELLO ◽  
Flávio de Mattos OLIVEIRA ◽  
Luiz Carlos SEVERO ◽  
...  
Keyword(s):  
Candida Species ◽  
Germ Tube ◽  
Mortality Rates ◽  
Correct Identification ◽  
Kappa Index ◽  
Candida Spp ◽  
Chromogenic Medium ◽  
Tube Test ◽  
Automated Method ◽  
Manual Methods

SUMMARY Infection by Candidaspp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candida spp. by the manual methods germ tube test, auxanogram and chromogenic medium in relation to the ID 32C automated method. The concordances between the methods in ascending order, measured by the Kappa index were: ID 32C with CHROMagar Candida(κ = 0.38), ID 32C with auxanogram (κ = 0.59) and ID 32C with germ tube (κ = 0.9). One of the species identified in this study was C. tropicalis,which demonstrated a sensitivity of 46.2%, a specificity of 95.2%, PPV of 80%, NPV of 81.1%, and an accuracy of 80.9% in tests performed with CHROMagar Candida;and a sensitivity of 76.9%, a specificity of 96.8%, PPV of 90.9%, NPV of 91%, and an accuracy of 91% in the auxanogram tests. Therefore, it is necessary to know the advantages and limitations of methods to choose the best combination between them for a fast and correct identification of Candidaspecies.

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