A study to evaluate etiology of denture stomatitis and to determine interrelationship with trauma and candida albicans count

Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to denture stomatitis. The purpose of this study is to identify the etiology of denture stomatitis and to establish the role of trauma and fungal infection in denture stomatitis.Subjects wearing previously fabricated removable partial or complete denture who were diagnosed with denture stomatitis were included. It is a cross sectional questionnaire-based study, for mycological study swab sample was smeared, Sabouraud Dextrose Agar (SDA) was used as culture media. Germ tube test was used to identify Candida albicans. To test significance between different variablesKruskal-Wallis test was used. Out of 195 subjects’ slight stomatitis was present in 52.80% patients, followed by moderate stomatitis in 32.31% and no stomatitis in 2.56% subjects. Candida was present in 25.64% patients. Candida significantly more common in moderate stomatitis than others.This study has suggested that there is a significant relation in denture stomatitis and Candida albicans in north Indian population. No significant role of trauma was noticed. Strong correlation has been found in Denture hygiene, cleaning habits and Denture Stomatitis.

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The role of the cytoskeleton in the polarized growth of the germ tube in Candida albicans

Microbiology ◽

10.1099/13500872-140-2-271 ◽

1994 ◽

Vol 140 (2) ◽

pp. 271-280 ◽

Cited By ~ 61

Author(s):

T. Akashi ◽

T. Kanbe ◽

K. Tanaka

Keyword(s):

Candida Albicans ◽

Germ Tube ◽

Polarized Growth

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Role of Candida albicans in Denture Stomatitis

Indian Academy of Oral Medicine and Radiology ◽

10.5005/jp-journals-10011-1234 ◽

2011 ◽

Vol 23 ◽

pp. 617-619

Author(s):

Chetan Hoshing ◽

Santosh Dixit ◽

Ajay Mootha ◽

Nikhil Diwan

Keyword(s):

Candida Albicans ◽

Denture Stomatitis

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Potential role of Candida albicans germ tube antibody in the diagnosis of deep-seated candidemia

Medical Mycology ◽

10.1093/mmy/myt025 ◽

2014 ◽

Vol 52 (3) ◽

pp. 270-275 ◽

Cited By ~ 21

Author(s):

M. Carmen Martínez-Jiménez ◽

Patricia Muñoz ◽

Jesús Guinea ◽

Maricela Valerio ◽

Roberto Alonso ◽

...

Keyword(s):

Candida Albicans ◽

Germ Tube ◽

Potential Role

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Lethality of Candida strains as influenced by the host

Canadian Journal of Microbiology ◽

10.1139/m75-093 ◽

1975 ◽

Vol 21 (5) ◽

pp. 648-654 ◽

Cited By ~ 27

Author(s):

Cora G. Saltarelli ◽

Kathi Ann Gentile ◽

Susan C. Mancuso

Keyword(s):

Candida Albicans ◽

Statistical Analysis ◽

Comparative Study ◽

Proteolytic Activity ◽

Germ Tube ◽

Culture Media ◽

Tube Formation ◽

Nutritional Requirements ◽

Morphological Mutant ◽

Mutant Strains

A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydospore production, germ tube formation, nor nutritional requirements was related to pathogenicity in mice. Statistical analysis indicated that the culture media of the organisms and the strain and sex of the mice into which the cells were injected were important factors in the development of pathogenicity.

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Defects in Assembly of the Extracellular Matrix Are Responsible for Altered Morphogenesis of a Candida albicans phr1 Mutant

Journal of Bacteriology ◽

10.1128/jb.180.1.163-166.1998 ◽

1998 ◽

Vol 180 (1) ◽

pp. 163-166 ◽

Cited By ~ 29

Author(s):

Laura Popolo ◽

Marina Vai

Keyword(s):

Saccharomyces Cerevisiae ◽

Candida Albicans ◽

Parental Strain ◽

Germ Tube ◽

Null Mutant ◽

Yeast Form ◽

Insoluble Glucan ◽

Cross Linker ◽

External Ph

ABSTRACT Analysis of Candida albicans cells using antibodies directed against Gas1p/Ggp1p, Saccharomyces cerevisiaehomolog of Phr1p, revealed that Phr1p is a glycoprotein of about 88 kDa whose accumulation increases with the rise of external pH. This polypeptide is present both in the yeast form and during germ tube induction. In the Phr1− cells at pH 8 the solubility of glucans in alkali is greatly affected. In the parental strain the alkali-soluble/-insoluble glucan ratio shows a 50% decrease at pH 8 with respect to pH 4.5, whereas in the null mutant it is unchanged, indicating the lack of a polymer cross-linker activity induced by the rise of pH. The mutant has a sixfold increase in chitin level and is hypersensitive to calcofluor. Consistently with a role of chitin in strengthening the cell wall, Phr1− cells are more sensitive to nikkomycin Z than the parental strain.

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PREVALENCE OF CANDIDA ASSOCIATED DENTURE STOMATITIS (CADS) AND SPECIATION OF CANDIDA AMONG COMPLETE DENTURE WEARERS OF SOUTH WEST COASTAL REGION OF KARNATAKA

Journal of Health and Allied Sciences NU ◽

10.1055/s-0040-1703679 ◽

2013 ◽

Vol 03 (03) ◽

pp. 059-063

Author(s):

Vinaya Bhat ◽

S.M. Sharma ◽

Veena Shetty ◽

C.S. Shastry ◽

Vaman Rao ◽

...

Keyword(s):

Candida Albicans ◽

Coastal Region ◽

Cross Sectional Survey ◽

Candida Spp ◽

Cross Sectional ◽

Denture Stomatitis ◽

Main Species ◽

South West ◽

Causative Agents ◽

Favorable Conditions

AbstractA denture wearer is highly prone to develop Candida associated denture stomatitis (CADS), due to the conversion of the normal oral commensal Candida spp. into a pathogen under favorable conditions. Immuno-compromised status, trauma from the prosthesis, other systemic conditions and improper maintenance of the dentures by the patient are few of the causative agents which turn the oral balance into an unhealthy and unsuitable foundation for the wearing of the prosthesis. Prevalence of denture stomatitis caused by the fungi Candida has been identified worldwide and has been reported to be around 65 to 70% of the denture wearers. The main species that causes infection has been Candida albicans, however, recently, there is a shift to non-Candida albicans-Candida (NCAC).This cross-sectional survey was undertaken to find the prevalence of denture stomatitis and the causative species in the south westcoastal region of Karnataka.

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Germ-tube production by Candida albicans in minimal liquid culture media

Canadian Journal of Microbiology ◽

10.1139/m71-137 ◽

1971 ◽

Vol 17 (7) ◽

pp. 851-856 ◽

Cited By ~ 19

Author(s):

D. N. Mardon ◽

I. S. K. Hurst ◽

E. Balish

Keyword(s):

Candida Albicans ◽

Butyric Acid ◽

Germ Tube ◽

Culture Medium ◽

Culture Media ◽

Tube Formation ◽

Gamma Amino Butyric Acid ◽

Tube Production ◽

Amino Butyric Acid ◽

Germ Tubes

Candida albicans formed germ tubes within 3 h at 37C in a glucose–salts–biotin (GSB) medium containing L-alpha-amino-n-butyric acid as the nitrogen source. Optimal germ-tube production was obtained when the inoculum was grown on Sabouraud dextrose agar. The GSB medium containing L-alpha-amino-n-butyric acid promoted germ-tube formation more effectively than GSB medium plus gamma-amino-butyric acid or Sabouraud dextrose broth.Carbon-14 incorporation studies revealed that during germ-tube formation (0–4 h) the 3 carbon of alpha-amino-n-butyric acid was incorporated intracellularly to a greater extent than the 1 carbon. However, during blastospore formation (5–16 h), this difference was less pronounced.When six other Candida species were grown in GSB plus L-alpha-amino-n-butyric acid medium, few germ tubes were observed with the exception of one Candida stellatoidea strain. However, even this strain of C. stellatoidea produced far fewer germ tubes in this minimal culture medium than any strain of C. albicans tested.

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