A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria

2020 ◽  
Author(s):  
Chika Shono ◽  
Dwi Ariyanti ◽  
Koichi Abe ◽  
Yuta Sakai ◽  
Ippei Sakamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Expression System ◽  
Green Light ◽  
T7 Rna Polymerase ◽  
Polymerase Gene ◽  
Gene Expression System ◽  
Rna Polymerase Gene

scholarly journals The optimization of cytoplasmic gene expression system with T7 RNA polymerase.

1998 ◽  
Vol 13 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Ryo Suzuki ◽  
Tetsuhiko Nakagawa ◽  
Hiroyuki Mizuguchi ◽  
Susumu Imazu ◽  
Tsuyoshi Nakanishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Gene Expression System

A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium

2009 ◽  
Vol 82 (6) ◽  
pp. 1195-1203 ◽  
Author(s):  
Martin Gamer ◽  
David Fröde ◽  
Rebekka Biedendieck ◽  
Simon Stammen ◽  
Dieter Jahn
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Bacillus Megaterium ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Gene Expression System

Establishment of a coupled expression system mediated by modified T7 RNA polymerase gene

2002 ◽  
Vol 47 (14) ◽  
pp. 1197-1201
Author(s):  
Feng Chen ◽  
Zixian Lu ◽  
Tuanjie Chang ◽  
Honglin Xu ◽  
Qian Wu ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Polymerase Gene ◽  
Rna Polymerase Gene

scholarly journals Artificial complementary chromatic acclimation gene expression system in Escherichia coli

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Dwi Ariyanti ◽  
Kazunori Ikebukuro ◽  
Koji Sode
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Light Exposure ◽  
Expression System ◽  
Red Light ◽  
Green Light ◽  
Light Illumination ◽  
Multiple Gene ◽  
Gene Expression System ◽  
Chromatic Acclimation

Abstract Background The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS/CcaR green light gene expression system derived from Synechocystis sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate cpcG2 promoter, and the constitutively transcribed promoter, the PtrcΔLacO promoter. Results One G-box was inserted upstream of the cpcG2 promoter and a reporter gene, the rfp gene (green light-induced gene expression), and the other G-box was inserted between the PtrcΔLacO promoter and a reporter gene, the bfp gene (red light-induced gene expression). The Escherichia coli transformants with plasmid-encoded genes were evaluated at the transcriptional and translational levels under red or green light illumination. Under green light illumination, the transcription and translation of the rfp gene were observed, whereas the expression of the bfp gene was repressed. Under red light illumination, the transcription and translation of the bfp gene were observed, whereas the expression of the rfp gene was repressed. During the red and green light exposure cycles at every 6 h, BFP expression increased under red light exposure while RFP expression was repressed, and RFP expression increased under green light exposure while BFP expression was repressed. Conclusion An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp. PCC6803, in E. coli. The artificial CCA system functioned repeatedly during red and green light exposure cycles. These results demonstrate the potential application of this CCA gene expression system for the production of multiple metabolites in a variety of microorganisms, such as cyanobacteria.

Overexpression of HumanN-Myristoyltransferase Utilizing a T7 Polymerase Gene Expression System

1996 ◽  
Vol 7 (4) ◽  
pp. 431-437 ◽  
Author(s):  
Rajala V.S. Raju ◽  
Raju S.S. Datla ◽  
Rajendra K. Sharma
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Polymerase Gene ◽  
Gene Expression System ◽  
T7 Polymerase
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