scholarly journals A self-initiating eukaryotic transient gene expression system based on cotransfection of bacteriophage T7 RNA polymerase and DNA vectors containing a T7 autogene

1994 ◽  
Vol 22 (11) ◽  
pp. 2114-2120 ◽  
Author(s):  
Xiaozhuo Chen ◽  
Yunsheng Li ◽  
Keyong Xiong ◽  
Thomas E. Wagner
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Expression System ◽  
Transient Gene Expression ◽  
T7 Rna Polymerase ◽  
Bacteriophage T7 ◽  
Gene Expression System ◽  
Dna Vectors

scholarly journals The optimization of cytoplasmic gene expression system with T7 RNA polymerase.

1998 ◽  
Vol 13 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Ryo Suzuki ◽  
Tetsuhiko Nakagawa ◽  
Hiroyuki Mizuguchi ◽  
Susumu Imazu ◽  
Tsuyoshi Nakanishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Gene Expression System

A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria

2020 ◽  
Author(s):  
Chika Shono ◽  
Dwi Ariyanti ◽  
Koichi Abe ◽  
Yuta Sakai ◽  
Ippei Sakamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Expression System ◽  
Green Light ◽  
T7 Rna Polymerase ◽  
Polymerase Gene ◽  
Gene Expression System ◽  
Rna Polymerase Gene

A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium

2009 ◽  
Vol 82 (6) ◽  
pp. 1195-1203 ◽  
Author(s):  
Martin Gamer ◽  
David Fröde ◽  
Rebekka Biedendieck ◽  
Simon Stammen ◽  
Dieter Jahn
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Bacillus Megaterium ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Gene Expression System

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Transient Gene Expression System Using Protoplasts from Developing Soybean Cotyledons.

1993 ◽  
Vol 43 (1) ◽  
pp. 53-60
Author(s):  
Mayuko ISHIKAWA ◽  
Kyuya HARADA ◽  
Fukumi SAKAI ◽  
Yuko OHASHI ◽  
Tadao NAITO
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Transient Gene Expression ◽  
Gene Expression System
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