Establishment of a coupled expression system mediated by modified T7 RNA polymerase gene

ABSTRACT Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.

Download Full-text

A mutation in the putative RNA polymerase gene inhibits nonhomologous, but not homologous, genetic recombination in an RNA virus

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.94.5.2073 ◽

1997 ◽

Vol 94 (5) ◽

pp. 2073-2078 ◽

Cited By ~ 45

Author(s):

M. Figlerowicz ◽

P. D. Nagy ◽

J. J. Bujarski

Keyword(s):

Rna Polymerase ◽

Genetic Recombination ◽

Rna Virus ◽

Polymerase Gene ◽

Rna Polymerase Gene

Download Full-text

Antisense oligonucleotides directed against the viral RNA polymerase gene enhance survival of mice infected with influenza A

Nature Biotechnology ◽

10.1038/9893 ◽

1999 ◽

Vol 17 (6) ◽

pp. 583-587 ◽

Cited By ~ 41

Author(s):

Tadashi Mizuta ◽

Masatoshi Fujiwara ◽

Toshifumi Hatta ◽

Takayuki Abe ◽

Naoko Miyano-Kurosaki ◽

...

Keyword(s):

Rna Polymerase ◽

Antisense Oligonucleotides ◽

Influenza A ◽

Viral Rna ◽

Polymerase Gene ◽

Rna Polymerase Gene

Download Full-text

Analysis of the 5′-Upstream Region of the Chloroplast RNA Polymerase Gene (rpoB)

Photosynthesis: from Light to Biosphere ◽

10.1007/978-94-009-0173-5_597 ◽

1995 ◽

pp. 2539-2542

Author(s):

Hitoshi Inada ◽

Motoaki Seki ◽

Hiromichi Morikawa ◽

Mitsuo Nishimura ◽

Koh Iba

Keyword(s):

Rna Polymerase ◽

Upstream Region ◽

Polymerase Gene ◽

Chloroplast Rna ◽

Rna Polymerase Gene ◽

Chloroplast Rna Polymerase

Download Full-text

Phylogenetic analysis of norovirus isolates involved in some Canadian gastroenteritis outbreaks in 2004 and 2005

Canadian Journal of Microbiology ◽

10.1139/w07-079 ◽

2007 ◽

Vol 53 (10) ◽

pp. 1133-1140 ◽

Cited By ~ 5

Author(s):

Safaa Lamhoujeb ◽

Hugues Charest ◽

Ismail Fliss ◽

Solange Ngazoa ◽

Julie Jean

Keyword(s):

Rna Polymerase ◽

Genetic Recombination ◽

Phylogenetic Analyses ◽

Sequence Information ◽

Viral Gastroenteritis ◽

Polymerase Gene ◽

Predominant Genotype ◽

Common Cause ◽

Actual Classification ◽

Rna Polymerase Gene

Noroviruses are recognized as the most common cause of nonbacterial gastroenteritis worldwide. In this study, we investigated the molecular epidemiology of noroviral isolates in Canada from 2004 to 2005 by sequencing the RNA polymerase gene and capsid N-terminal/shell (N/S) domain. Norovirus genogroups I and II were thus found to have co-circulated in Canada during the studied period, with a higher incidence of genogroup II (95.7%). The GII-4 or Lordsdale subgroup was the predominant genotype, suggesting that norovirus genogroup II is the major cause of viral gastroenteritis in Canada, as it is in many other countries. Phylogenetic analyses of the RNA polymerase gene and the capsid N/S domain indicated different genotypes for 2 strains, suggesting probable genetic recombination. Sequencing of the norovirus polymerase gene may reflect actual classification but should be supported by sequence information obtained from the capsid gene.

Download Full-text

High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

Biochemical Society Transactions ◽

10.1042/bst0330056 ◽

2005 ◽

Vol 33 (1) ◽

pp. 56-58 ◽

Cited By ~ 9

Author(s):

T. Drepper ◽

S. Arvani ◽

F. Rosenau ◽

S. Wilhelm ◽

K.-E. Jaeger

Keyword(s):

Rna Polymerase ◽

Rhodobacter Capsulatus ◽

Expression System ◽

Active Form ◽

T7 Rna Polymerase ◽

High Level Synthesis ◽

Phototrophic Bacterium ◽

Large Gene ◽

High Level ◽

Complex Enzymes

High-level synthesis of complex enzymes like bacterial [NiFe] hydrogenases, in general, requires an expression system that allows concerted expression of a large number of genes. So far, it has not been possible to overproduce a hydrogenase in a stable and active form by using a customary expression system. Therefore we started to establish a new, T7-based expression system in the phototrophic bacterium Rhodobacter capsulatus. The beneficial properties of this bacterial host in combination with the unique capacity of T7 RNA polymerase to synthesize long transcripts will allow the high-level synthesis and assembly of active hydrogenase as well as other complex enzymes in the near future.

Download Full-text