Overexpression of AIB1 correlates inversely with E-cadherin expression in pancreatic adenocarcinoma and may promote lymph node metastasis

ObjectiveThe aim of this study was to evaluate the prognostic value of E-cadherin, β-catenin, vimentin and S100A4 expression in a cohort of squamous cell lung carcinoma (SqCC) patients.MethodsTumours from 204 patients with surgically resected SqCC were used for the immunohistochemical analyses of E-cadherin, β-catenin, vimentin and S100A4 expression. Correlations between the expression of these markers and clinicopathological parameters were analysed using the χ2 test. The prognostic value of these markers was evaluated using univariate Kaplan–Meier survival analyses and multivariate Cox proportional hazards model analyses.ResultsSignificant associations between E-cadherin expression and T stage (p=0.040), histological differentiation (p=0.005), lymph node metastasis (p<0.001), and recurrence (p<0.001) were identified. Decreased β-catenin expression was significantly correlated with T stage (p=0.003) and lymph node metastasis (p=0.010). Vimentin expression was associated with histological differentiation (p=0.017) and lymph node metastasis (p=0.001). Moreover, significant correlations were observed between S100A4 expression and lymph node metastasis (p=0.020) and recurrence (p<0.001). In the univariate analyses, high E-cadherin expression was a positive indicator for overall survival (OS) (p<0.001) and disease-free survival (DFS) (p<0.001), whereas high S100A4 or vimentin expression were negative indicators for OS (p<0.001 and p=0.010, respectively) and DFS (p<0.001 and p=0.006, respectively). In the multivariate analyses, E-cadherin and S100A4 expression were independent prognostic factors for OS (HR 0.697, 95% CI 0.524 to 0.926, p=0.013, and HR 1.508, 95% CI 1.122 to 2.027, p=0.007, respectively) and DFS (HR 0.634, 95% CI 0.471 to 0.852, p=0.003, and HR 1.490, 95% CI 1.101 to 2.015, p=0.010, respectively).ConclusionsEffective analysis of E-cadherin and S100A4 expression may allow for the identification of patients who are at a high risk of recurrence and poor prognosis in SqCC.

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HMGA1 and HMGA2 protein expression correlates with advanced tumour grade and lymph node metastasis in pancreatic adenocarcinoma

Histopathology ◽

10.1111/j.1365-2559.2011.04121.x ◽

2012 ◽

Vol 60 (3) ◽

pp. 397-404 ◽

Cited By ~ 54

Author(s):

Salvatore Piscuoglio ◽

Inti Zlobec ◽

Pierlorenzo Pallante ◽

Romina Sepe ◽

Francesco Esposito ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Protein Expression ◽

Pancreatic Adenocarcinoma ◽

Tumour Grade ◽

Node Metastasis ◽

Hmga2 Protein

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Downregulation and abnormal expression of E-cadherin and β-catenin in nasopharyngeal carcinoma: Close association with advanced disease stage and lymph node metastasis

Human Pathology ◽

10.1016/s0046-8177(99)90123-5 ◽

1999 ◽

Vol 30 (4) ◽

pp. 458-466 ◽

Cited By ~ 91

Author(s):

Zhong Zheng ◽

Jie Pan ◽

Bin Chu ◽

Yong-Chuan Wong ◽

Annie Lai-Man Cheung ◽

...

Keyword(s):

Lymph Node ◽

Nasopharyngeal Carcinoma ◽

Lymph Node Metastasis ◽

Advanced Disease ◽

Close Association ◽

Disease Stage ◽

Node Metastasis ◽

Abnormal Expression ◽

Advanced Disease Stage ◽

E Cadherin

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Reduced E-Cadherin Expression Is Associated with Increased Lymph Node Metastasis and Unfavorable Prognosis in Non-small Cell Lung Cancer

American Journal of Respiratory and Critical Care Medicine ◽

10.1164/ajrccm.157.4.9703099 ◽

1998 ◽

Vol 157 (4) ◽

pp. 1319-1323 ◽

Cited By ~ 87

Author(s):

MAARTEN A. SULZER ◽

MATHIE P. G. LEERS ◽

JAN A. van NOORD ◽

EWALD C. M. BOLLEN ◽

PAUL H. M. H. THEUNISSEN

Keyword(s):

Lung Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

Node Metastasis ◽

E Cadherin

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Vimentin overexpression as a novel poor prognostic biomarker in eyelid sebaceous gland carcinoma

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2018-313285 ◽

2019 ◽

Vol 104 (6) ◽

pp. 879-884 ◽

Cited By ~ 1

Author(s):

Mansi Bhardwaj ◽

Seema Sen ◽

Kunzang Chosdol ◽

Sameer Bakhshi ◽

Neelam Pushker ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Sebaceous Gland ◽

Vimentin Expression ◽

Sebaceous Gland Carcinoma ◽

Node Metastasis ◽

Gland Carcinoma ◽

Advanced Tumour Stage ◽

Emt Markers ◽

E Cadherin

BackgroundVimentin is an intermediate-sized filament which is highly expressed in mesenchymal cells and is associated with epithelial–mesenchymal transition (EMT). EMT markers ZEB2 and Slug lead to Vimentin overexpression and E-cadherin loss, resulting in invasion and metastasis. However, the status of Vimentin remains unexplored in eyelid sebaceous gland carcinoma (SGC). The study aims to determine status of Vimentin in SGC and its association with EMT markers E-cadherin, ZEB2 and Slug.MethodsVimentin protein expression was undertaken in 66 cases with SGC by immunohistochemistry (IHC). Messenger RNA (mRNA) expression was determined in 42 fresh tissues by quantitative real-time PCR. Association of Vimentin with E-cadherin, ZEB2 and Slug was also analysed. Patients were followed up for 17–69 months (mean 34.02 ± 14.73 months).ResultsIHC revealed Vimentin overexpression in 37/66 (56%) cases. This overexpression showed significant association with lymph node metastasis (p=0.004) and pagetoid spread (p=0.05). Patients with high Vimentin expression also had poor disease-free survival (p=0.033). Univariate Cox regression model indicated that high Vimentin expression (p=0.043) and advanced tumour stage (p=0.002) were independent adverse prognostic factors. High Vimentin mRNA expression was seen in 16/42 (38%) cases and correlated significantly with lymph node metastasis (p=0.027), advanced tumour stage (p=0.002) and large tumour size (p=0.023). Vimentin expression overall showed a significant inverse association with E-cadherin and direct association with ZEB2 expression.ConclusionsVimentin overexpression in SGC is associated with EMT and leads to poor clinical outcome. It also emerged as a novel predictor for lymph node metastasis and poor survival.

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Loss of CDH1 (E-cadherin) expression is associated with infiltrative tumour growth and lymph node metastasis

British Journal of Cancer ◽

10.1038/bjc.2015.347 ◽

2016 ◽

Vol 114 (2) ◽

pp. 199-206 ◽

Cited By ~ 35

Author(s):

Sun A Kim ◽

Kentaro Inamura ◽

Mai Yamauchi ◽

Reiko Nishihara ◽

Kosuke Mima ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Tumour Growth ◽

Node Metastasis ◽

E Cadherin

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Prognostic and Risk Factors in Patients with Locally Advanced Cutaneous Squamous Cell Carcinoma of the Trunk and Extremities

Journal of Skin Cancer ◽

10.1155/2011/420796 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 11

Author(s):

de Lima Vazquez Vinicius ◽

Cristovam Scapulatempo ◽

Natalia Martins Perpetuo ◽

Faheez Mohamed ◽

Teóclito Sachetto de Carvalho ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Cell Carcinoma ◽

Squamous Cell ◽

Locally Advanced ◽

Cutaneous Squamous Cell Carcinoma ◽

Primary Tumors ◽

Node Metastasis ◽

E Cadherin

55 patients with advanced cutaneous squamous cell carcinoma (CSCC) of the trunk and extremities were studied.A Tissue Microarraywas constructed using immunohistochemistry to quantify expression of the HER family, E-cadherins, and podoplanin. Clinical and histopathological factors related to lymph node metastasis and prognosis were also established. Primary tumor positivity was 25.5% for EGFR, 87.3% for HER-3, and 48.1% for HER-4. Metastases were positive for EGFR in 41.7%, for HER-3 in 83.3%, and HER-4 in 43.5%. HER-2 was negative in all samples. Membrane E-cadherin and cytoplasmic E-cadherin were positive in 47.3% and 30.2% of primary tumors and 45.5% and 27.3% of metastases, respectively. Podoplanin was positive in 41.8% of primary tumors and 41.7% of metastases. Intratumoral lymphocytic infiltrate was associated with lymph node metastasis. Patients with T3 tumors had better cancer-specific survival (CSS) than those with T4 tumors; patients with no lymph node involvement had better CSS than patients with N1 tumors. Undifferentiated tumors and hyperexpression of podoplanin were negative prognostic indicators on multivariate analysis.

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