First Report of Pepper mottle virus on Capsicum annuum in Japan

2003 ◽  
Vol 69 (5) ◽  
pp. 348-350 ◽  
Author(s):  
Yoshihiro Ogawa ◽  
Kyoji Hagiwara ◽  
Hisashi Iwai ◽  
Shoichi Izumi ◽  
Kei Arai
Keyword(s):  
Capsicum Annuum ◽  
Mottle Virus ◽  
Pepper Mottle Virus ◽  
First Report

scholarly journals First Report of Pepper mottle virus Infecting Tomato in Hawaii

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 917-917 ◽  
Author(s):  
M. J. Melzer ◽  
J. S. Sugano ◽  
D. Cabanas ◽  
K. K. Dey ◽  
B. Kandouh ◽  
...  
Keyword(s):  
Field Trial ◽  
Consensus Sequence ◽  
The United States ◽  
Mottle Virus ◽  
Tomato Production ◽  
Body Protein ◽  
Pepper Mottle Virus ◽  
First Report ◽  
Asymptomatic Plant ◽  
Das Elisa

In August 2011, tomato (Solanum lycopersicum L.) fruit from a University of Hawaii field trial displayed mottling symptoms similar to that caused by Tomato spotted wilt virus (TSWV) or other tospoviruses. The foliage from affected plants, however, appeared symptomless. Fruit and leaf tissue from affected plants were negative for TSWV analyzed by double antibody sandwich (DAS)-ELISA and/or TSWV ImmunoStrips (Agdia, Elkhart, IN) when performed following the manufacturer's instructions. Total RNA from a symptomatic and an asymptomatic plant was isolated using an RNeasy Plant Mini Kit (Qiagen, Valencia, CA) and reverse transcribed using Invitrogen SuperScript III reverse transcriptase (Life Technologies, Grand Island, NY) and primer 900 (5′- CACTCCCTATTATCCAGG(T)16-3′) following the enzyme manufacturer's instructions. The cDNA was then used as template in a universal potyvirus PCR assay using primers 900 and Sprimer, which amplify sequences encoding the partial inclusion body protein (NIb), coat protein, and 3′ untranslated region of potyviruses (1). A ~1,700-bp product was amplified from the cDNA of the symptomatic plant but not the asymptomatic plant. This product was cloned using pGEM-T Easy (Promega, Madison, WI) and three clones were sequenced at the University of Hawaii's Advanced Studies in Genomics, Proteomics, and Bioinformatics laboratory. The 1,747-bp consensus sequence of the three clones was deposited in GenBank (Accession No. JQ429788) and, following primer sequence trimming, found to be 97% identical to positions 7,934 through 9,640 of Pepper mottle virus (PepMoV; family Potyviridae, genus Potyvirus) accessions from Korea (isolate ‘217’ from tomato; EU586126) and California (isolate ‘C’ from pepper; M96425). To determine the incidence of PepMoV in the field trial, all 292 plants representing 14 tomato cultivars were assayed for the virus 17 weeks after planting using a PepMoV-specific DAS-ELISA (Agdia) following the manufacturer's directions. Plants were considered positive if their mean absorbance at 405 nm was greater than the mean absorbance + 3 standard deviations + 10% of the negative control samples. The virus incidence ranged from 4.8 to 47.6% for the different varieties, with an overall incidence of 19.9%. Although plant growth was not noticeably impaired by PepMoV infection, the majority of fruit from infected plants was unsaleable, making PepMoV a considerable threat to tomato production in Hawaii. PepMoV has been reported to naturally infect tomato in Guatemala (3) and South Korea (2). To our knowledge, this is the first report of this virus in Hawaii and the first report of this virus naturally infecting tomato in the United States. References: (1) J. Chen et al. Arch. Virol. 146:757, 2001. (2) M.-K. Kim et al. Plant Pathol. J. 24:152, 2008. (3) J. Th. J. Verhoeven et al. Plant Dis. 86:186, 2002.

scholarly journals First Report of Tobacco mild green mosaic virus Infecting Capsicum annuum in Tunisia

Plant Disease ◽  
2009 ◽  
Vol 93 (7) ◽  
pp. 761-761 ◽  
Author(s):  
M. I. Font ◽  
M. C. Córdoba-Sellés ◽  
M. C. Cebrián ◽  
J. A. Herrera-Vásquez ◽  
A. Alfaro-Fernández ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Capsicum Annuum ◽  
Polyclonal Antibodies ◽  
Plant Viruses ◽  
Mottle Virus ◽  
Tomato Mosaic Virus ◽  
Rt Pcr ◽  
Tomato Plants ◽  
First Report ◽  
Pepper Plants

During the springs of 2007 and 2008, leaf deformations as well as symptoms of mild green and chlorotic mosaic were observed on pepper (Capsicum annuum) plants grown in Monastir (northwest Tunisia) and Kebili (southeast Tunisia). With the support of projects A/5269/06 and A/8584/07 from the Spanish Agency for International Cooperation (AECI), symptomatic leaf samples were analyzed by transmission electron microscopy (TEM) of leaf-dip preparations. Typical tobamovirus-like particles (rigid rods ≈300 nm long) were observed in crude plant extracts. According to literature, at least six tobamoviruses infect peppers: Paprika mild mottle virus (PaMMV); Pepper mild mottle virus (PMMoV); Ribgrass mosaic virus (RMV); Tobacco mild green mosaic virus (TMGMV); Tobacco mosaic virus (TMV); and Tomato mosaic virus (ToMV) (1). Extracts from six symptomatic plants from Monastir and four from Kebili fields tested negative for ToMV, TMV, and PMMoV and tested positive for TMGMV by double-antibody sandwich (DAS)-ELISA using polyclonal antibodies specific to each virus (Loewe Biochemica GMBH, Sauerlach, Germany). To confirm the positive TMGMV results, total RNAs from 10 symptomatic plants that tested positive by ELISA were extracted and analyzed by reverse transcription (RT)-PCR using primers designed to specifically amplify a region of the coat protein gene (CP) of TMGMV (2). The 524-bp TMGMV-CP specific DNA fragment was amplified from all samples, but was not amplified from healthy plants or the sterile water used with negative controls. RT-PCR products were purified and directly sequenced. BLAST analysis of the obtained sequence (GenBank No. EU770626) showed 99 to 98% nucleotide identity with TMGMV isolates PAN-1, DSMZ PV-0113, TMGMV-Pt, and VZ1 (GenBank Nos. EU934035, EF469769, AM262165, and DQ460731, respectively) and less than 69% with PaMMV and PMMoV isolates (GenBank Nos. X72586 and AF103777, respectively). Two TMGMV-positive, singly, infected symptomatic pepper plants collected from Monastir and Kebili were used in mechanical transmissions to new pepper and tomato plants. Inoculated pepper plants exhibited mild chlorosis symptoms and tested positive for TMGMV only; however, inoculated tomato plants cv. Marmande were asymptomatic and tested negative as expected for TMGMV infection (1). To our knowledge, although C. annuum has been shown as a natural host for TMGMV (2), this is the first report of TMGMV in Tunisia. Reference: (1) A. A. Brunt et al. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20th August 1996. Online publication, 1996. (2) J. Cohen et al. Ann. Appl. Biol. 138:153, 2001.

scholarly journals First report of Bell pepper mottle virus infecting Calibrachoa in Germany

2019 ◽  
Vol 39 ◽  
pp. 15
Author(s):  
W. Menzel ◽  
S. Winter ◽  
J. Hamacher ◽  
M. Heupel
Keyword(s):  
Bell Pepper ◽  
Mottle Virus ◽  
Pepper Mottle Virus ◽  
First Report

scholarly journals First Report of Apple of Peru (Nicandra physalodes) Infected with Pepper Mottle Virus in Hawaii

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 169-169
Author(s):  
D. Wang ◽  
I. Hamim ◽  
W. B. Borth ◽  
M. J. Melzer ◽  
J. Y. Suzuki ◽  
...  
Keyword(s):  
Mottle Virus ◽  
Pepper Mottle Virus ◽  
First Report ◽  
Nicandra Physalodes

scholarly journals First Report of Pepper mottle virus in Tomato

Plant Disease ◽  
2002 ◽  
Vol 86 (2) ◽  
pp. 186-186 ◽  
Author(s):  
J. Th. J. Verhoeven ◽  
T. M. Willemen ◽  
J. W. Roenhorst
Keyword(s):  
Nucleotide Sequence ◽  
Lycopersicon Esculentum ◽  
Potato Virus ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Degenerate Primers ◽  
Pepper Mottle Virus ◽  
First Report ◽  
Transmission Electron ◽  
Nontranslated Region

In 2000, a breeding company submitted a tomato (Lycopersicon esculentum) sample from Guatemala for diagnosis. The plants showed necrotic lesions on leaves surrounded by some chlorosis, necrotic streaks on stems, and large superficial necrotic lesions on fruits. By mechanical inoculation of plant sap to different plant species, symptoms appeared in Capsicum annuum ‘Westlandse Grote Zoete’, Lycopersicon esculentum ‘Money-maker’, Nicotiana benthamiana, N. bigelovii, N. glutinosa, N. hesperis-67A, N. occidentalis-P1, N. tabacum ‘White Burley’, and Physalis floridana. Systemically infected leaves from N. occidentalis-P1 were used for all further experiments. Leaf dip preparations were analyzed by transmission electron microscopy and revealed the presence of filamentous virus particles typical of a potyvirus. Double-antibody sandwich enzyme-linked immunosorbent assay tests for Potato virus A, V, and Y, Tobacco etch virus, and Wild potato mosaic virus were negative. An antiserum (PepMoV/DSMZ As 0186) to Pepper mottle virus (PepMoV), however, gave a positive reaction. To obtain further evidence for the presence of this virus, the nucleotide sequence of the complete 3′ nontranslated region (3NTR) and the 3′ terminal part of the coat protein gene (3CPG) was determined using the set of degenerate primers P9502/CPUP (1). The obtained nucleotide sequence (approximately 700 bp) was deposited in GenBank under Accession No. AF440801. It showed 93 to 94% 3NTR and 90 to 93% 3CPG homology with the three sequences of PepMoV from pepper already present in GenBank. The two viruses with the next closest nucleotide sequence homology were Potato virus V and Potato virus Y showing up to 80 and 75% homology with the 3CPG and up to 53 and 48% homology with the 3NTR, respectively. Based on these results, we concluded that the virus isolated from the symptomatic tomato plants was PepMoV. Because of the relatively low homologies with the pepper isolates of PepMoV, this tomato isolate might be a separate strain of the virus. To our knowledge, this is the first report of the occurrence of PepMoV in tomato. Reference: (1) R. A. A. van der Vlugt et al. Phytopathology 89:148, 1999.

scholarly journals First Report of Pepper mottle virus in Bell Pepper in Taiwan

Plant Disease ◽  
2011 ◽  
Vol 95 (5) ◽  
pp. 617-617 ◽  
Author(s):  
Y. H. Cheng ◽  
T. C. Deng ◽  
C. C. Chen ◽  
J. Y. Liao ◽  
C. A. Chang ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
Chenopodium Quinoa ◽  
Bell Pepper ◽  
Mottle Virus ◽  
Rt Pcr ◽  
Degenerate Primers ◽  
Pepper Mottle Virus ◽  
First Report ◽  
Bell Peppers ◽  
Das Elisa

Bell pepper (Capsicum annuum L.) plants exhibiting systemic mild mosaic, vein yellowing, and leaf malformation were collected from Puli City in 2006. Double-antibody sandwich (DAS)-ELISA was used to test these samples for Chilli veinal mottle virus (ChiVMV) infection using polyclonal antibodies. In addition, Chenopodium quinoa, C. amaranticolor, and Nicotiana benthamiana plants were mechanically inoculated with sap extracted from collected samples. Ten days postinoculation, chlorotic local lesions were observed on inoculated leaves of C. quinoa and C. amaranticolor plants, whereas, systemic mosaic and foliar distortion symptoms were developed on upper leaves of N. benthamiana plants. The DAS-ELISA test showed that field-collected pepper samples and inoculated leaves of C. quinoa and C. amaranticolor were infected with ChiVMV, while N. benthamiana with mosaic symptoms did not react with ChiVMV antibodies. To confirm ChiVMV, field-collected samples as well as mechanically inoculated plants were tested by reverse transcription (RT)-PCR using the potyvirus degenerate primers Hrp5/Pot1 (2). Amplified RT-PCR products were cloned and sequenced. Sequence analysis of amplified fragments (1.4 kb) revealed that field-collected pepper samples were infected with ChiVMV and Pepper mottle virus (PepMoV). The DNA fragment amplified from C. quinoa and C. amaranticolor showed high (99.2%) sequence identities with the CP gene of ChiVMV (3) (GenBank Accession No. AM909717). However, amplicons obtained from N. benthamiana plants (GenBank Accession No. HQ329082) that showed mosaic symptoms showed 83.6% to 98.7% nucleotide identities with PepMoV (GenBank Accession Nos. AB126033, AF227728, AF440801, AF501591, EU586133, and M96425). Next, a pure isolate of PepMoV was established on N. benthamiana by mechanical inoculation of diluted plant sap obtained from a PepMoV-infected N. benthamiana plant. Bell pepper plants inoculated with the Taiwan isolate of PepMoV developed mosaic and leaf distortion symptoms. Antiserum against the PepMoV Taiwan isolate was subsequently prepared by immunizing rabbits with purified virus particles. Using the prepared antiserum and specific primers (1) to detect PepMoV, ChiVMV, and Pepper veinal mottle virus (PVMV), three viruses could be readily detected and differentiated from diseased bell peppers in the field. In a survey done in 2007, 18 of 33 pepper samples from southern Taiwan were found with mixed infections of PepMoV and ChiVMV, seven samples were infected with PepMoV and PVMV, five samples were infected with PVMV, and another three samples were infected with ChiVMV. To our knowledge, this is the first report of the occurrence of PepMoV in bell peppers in Taiwan. References: (1) Y. H. Cheng et al. Plant Dis. 93:107, 2009. (2) S. S. Pappu et al. Plant Dis. 82:1121, 1998. (3) W. S. Tsai et al. Plant Pathol. 58:408, 2008.

scholarly journals Inhibitory Effect of Cheese Whey on Cucumber Mosaic Virus and Pepper Mottle Virus in Capsicum annuum

2020 ◽  
Vol 26 (2) ◽  
pp. 103-108
Author(s):  
Bong Nam Chung ◽  
Sun Jung Kwon ◽  
Gug Seoun Choi ◽  
Ju Yeon Yoon ◽  
In Sook Cho
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Capsicum Annuum ◽  
Cheese Whey ◽  
Inhibitory Effect ◽  
Mottle Virus ◽  
Pepper Mottle Virus
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