scholarly journals Inhibitory Effect of Cheese Whey on Cucumber Mosaic Virus and Pepper Mottle Virus in Capsicum annuum

2020 ◽  
Vol 26 (2) ◽  
pp. 103-108
Author(s):  
Bong Nam Chung ◽  
Sun Jung Kwon ◽  
Gug Seoun Choi ◽  
Ju Yeon Yoon ◽  
In Sook Cho
2018 ◽  
Vol 3 (2) ◽  
pp. 381-390
Author(s):  
Rodolfo Velásquez-Valle ◽  
Luis Roberto Reveles-Torres ◽  
Jaime Mena-Covarrubias

A nivel mundial el cultivo de chile es afectado por más de 60 enfermedades virales; sin embargo, poco se conoce acerca de ellas en el área productora de chile seco del norte centro de México por lo que el objetivo del presente trabajo consistió en detectar la presencia y sintomatología de cinco virus en parcelas comerciales de chile seco en los estados mencionados. Plantas de chile de los tipos mirasol y ancho fueron muestreadas y se anotó la presencia de síntomas como enanismo, clorosis, deformación de hojas, defoliación, necrosis vascular y ramas unidas. Las muestras fueron analizadas mediante la técnica DAS- ELISA empleando los antisueros para el virus del mosaico del tabaco (Tobacco mosaic virus: TMV), mosaico del pepino (Cucumber mosaic virus: CMV), Y de la papa (Potato virus Y: PVY), moteado del chile (Pepper mottle virus: PepMoV) y jaspeado del tabaco (Tobacco etch virus: TEV). Esos virus fueron identificados en plantas de chile colectadas en las parcelas comerciales de chile seco de los tres estados antes mencionados.


2006 ◽  
Vol 96 (3) ◽  
pp. 240-247 ◽  
Author(s):  
John F. Murphy ◽  
Kira L. Bowen

The occurrence of more than one virus species in a single plant is not uncommon in cultivated and native plant species. A mixed virus infection may lead to greater disease severity than individual viral components and this is sometimes referred to as a synergistic disease. Although, in some cases, synergism has been demonstrated for various plant growth parameters such as plant height, weight, and yield, proof of synergy typically has not been demonstrated for symptom severity when the mixed virus infection was not lethal. We demonstrated synergy in bell pepper plants co-infected with Cucumber mosaic virus (CMV) and Pepper mottle virus (PepMoV) relative to each virus alone for stem height (two of three trials) and aboveground fresh weight (one of three trials) using factorial analysis and Abbott's equation for synergy. This approach allowed affirmation of the type of response (i.e., synergistic rather than antagonistic) and statistical proof of synergy. A detailed evaluation of symptom severity for each viral treatment revealed three phases associated with host plant developmental stages. A numerical symptom severity rating scale was developed and used in each of two equations to demonstrate statistical proof for synergy based on symptom severity for co-infected plants. Virus accumulation in noninoculated leaves was determined by enzyme-linked immunosorbent assay. In singly infected plants, CMV titers declined in mildly symptomatic leaves representing later stages of plant development, but titers increased in similar leaves of co-infected plants. In contrast, PepMoV titers did not differ in singly or co-infected plants.


2021 ◽  
Vol 37 (6) ◽  
pp. 632-640
Author(s):  
Venkata Subba Reddy Gangireddygari ◽  
Bong Nam Chung ◽  
In-Sook Cho ◽  
Ju-Yeon Yoon

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material – chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.


2006 ◽  
Vol 13 (2) ◽  
pp. 53-57 ◽  
Author(s):  
DWI SUBEKTI ◽  
SRI HENDRASTUTI HIDAYAT ◽  
ENDANG NURHAYATI ◽  
SRIANI SUJIPRIHATI

Author(s):  
Pedro Valadez-Ramírez ◽  
Javier Paz-Román ◽  
Salvador Guzmán-González ◽  
Marco Tulio Buenrostro-Nava ◽  
Daniel Leobardo Ochoa-Martínez

El <em>Cucumber mosaic virus</em> (CMV) ocasiona una de las enfermedades virales más importantes a nivel mundial en plantas silvestres y cultivadas. En México son pocos los estudios que se han abordado con este virus, y dada su amplia gama de hospedantes e impacto económico, es necesario contar con mayor información de su presencia y distribución en zonas de importancia agrícola como las del estado de Colima. En este trabajo, se reportan nuevos aislamientos del CMV identificados por RT-PCR, secuenciación de DNA y su análisis filogenético: CMV-Vin en vinca (<em>Catharanthus roseus</em>), CMV-Chi en chile jalapeño (<em>Capsicum annuum</em>) y CMV-Tom en tomate saladette (<em>Solanum lycopersicum</em>). Se confirmó, además, la presencia del CMV en melón cantaloupe (<em>Cucumis melo</em>) (CMV-Mel). Los aislamientos CMV-Vin, CMV-Chi y CMV-Mel agruparon en el subgrupo IB, mientras que CMV-Tom agrupó en el subgrupo IA de CMV. De estos aislamientos, sólo CMV-Vin evidenció la presencia de un RNA satélite (satRNA Vin) sin dominio necrogénico. Este es el primer reporte de la presencia del CMV en vinca, chile y tomate y de un RNA satélite en vinca en Colima, México.


2012 ◽  
Vol 7 (2) ◽  
pp. 130-139
Author(s):  
Muhammad Taufik ◽  
Sri Hendrastuti Hidayat ◽  
Sriani Sujiprihati ◽  
Gede Suastika ◽  
Sientje Mandang Sumaraw

Resistance Evaluation of Chillipepper Cultivars for Cucumber Mosaic Virus and Chilli Veinal Mottle Virus.  The use of resistance culivars is an important strategy for management of virus infection in chillipepper. A research was undergone to study the effect of single and mix infection of CMV and ChiVMV on the disease incidence and on the growth and yield of nine chillipepper cultivars, i.e. Cilibangi 4, Cilibangi 5, Cilibangi 6, Helem, Jatilaba, Tit Bulat, Tit Segitiga, Tit Super and Tampar. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA.  In general, inoculated chillipepper cultivars developed similar symptoms, i.e. mosaic type for CMV and mottle type for ChiVMV.  More severe symptom was not always observed from mix infection of CMV and ChiVMV. Disease incidence occurred in the range of 16.67 – 86.0% and this caused 18.3 – 98.6% yield loss.  Based on symptom expression, ELISA result, and reduction on yield, it can be concluded that all chillipepper cultivars used in this study could not hold up the virus infection. However, several cultivars showed tolerance response :  Jatilaba, Tit Super, and Tampar for CMV; Cilibangi 4 for ChiVMV; Tit Super for mix infection; and Cilibangi 5 for CMV, ChiVMV, and mix infection.  Further evaluation and investigation involving different chillipepper cultivars should be conducted.


2010 ◽  
Vol 2 (1) ◽  
Author(s):  
Olawale Arogundade ◽  
Olusegun Samuel Balogun ◽  
Olumide Shokalu ◽  
Taiye Hussein Aliyu

2003 ◽  
Vol 69 (5) ◽  
pp. 348-350 ◽  
Author(s):  
Yoshihiro Ogawa ◽  
Kyoji Hagiwara ◽  
Hisashi Iwai ◽  
Shoichi Izumi ◽  
Kei Arai

Sign in / Sign up

Export Citation Format

Share Document