Phenotypic characterization of Pseudomonas fluorescens PfG32R and its spontaneous gacS mutants and biocontrol activity against bacterial wilt disease of tomato

2006 ◽  
Vol 72 (3) ◽  
pp. 168-175 ◽  
Author(s):  
W. Gusti Ngurah Alit-Susanta ◽  
Yuichi Takikawa
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Wilt Disease ◽  
Phenotypic Characterization ◽  
Bacterial Wilt Disease ◽  
Biocontrol Activity

scholarly journals Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam pengendalian hayati Ralstonia solanacearum penyebab penyakit layu bakteri pada tomat

Jurnal Agro ◽  
10.15575/2305 ◽  
2018 ◽  
Vol 5 (1) ◽  
pp. 1-12
Author(s):  
Istiqomah Istiqomah ◽  
Dian Eka Kusumawati
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Total Phenol ◽  
Tomato Production ◽  
Bacterial Wilt Disease ◽  
Type Test ◽  
Important Disease

Salah satu penyakit penting pada produksi tomat di Indonesia adalah layu bakteri yang disebabkan oleh Ralstonia solanacearum. Alternatif untuk mengendalikan penyakit layu bakteri adalah dengan menggunakan Bacillus subtilis dan Pseudomonas fluorescens. Tujuan penelitian ini untuk mengetahui kemampuan B. subtilis dan P. fluorescens dalam mengendalikan penyakit layu bakteri yang disebabkan R. solanacearum serta mekanisme penghambatannya. Penelitian ini terdiri dari 5 tahap, yaitu perbanyakan inokulum R. solanacearum, uji virulensi dan uji hipersensitif  R. solanacearum, uji antagonis B. subtilis dan P. fluorescens terhadap R. solanacearum pada media agar, uji jenis antibiosis, penelitian di rumah kaca, dan analisis total fenol. Hasil penelitian uji antagonis menunjukkan bahwa semua isolat B. subtilis dan P. fluorescens memiliki potensi menghambat R. solanacearum dengan tipe antibiosis bakteriostatik. Hasil analisis kadar fenol menunjukkan bahwa terjadi peningkatan total fenol secara signifikan pada tanaman tomat yang diaplikasikan isolat B. subtilis UB-ABS6, P. fluorescens UB-PF5 dan P. fluorescens UB-PF6. Penelitian di rumah kaca menunjukkan bahwa semua tanaman tomat yang diaplikasikan agens hayati mengalami penundaan masa inkubasi dibandingkan dengan kontrol. Isolat B. subtilis UB-ABS2, B. subtilis UB-ABS6, P. fluorescens UB-PF5 dan P. fluorescens UB-PF6 secara signifikan menekan kejadian penyakit layu bakteri berturut-turut 50%, 30%, 60%, dan 60%. B. subtilis dan P. fluorescens dapat dimanfaatkan untuk mengendalikan layu bakteri pada tomat yang disebabkan oleh Ralstonia solanacearum. One of important disease that infects tomato production in Indonesia is bacterial wilt disease caused by Ralstonia solanacearum. Alternative on controlling bacterial wilt is using Bacillus subtilis and Pseudomonas fluorescens. Goal of the research was to find out ability of B. subtilis and P. fluorescens to control R. Solanacearum and mechanism of the inhibition. This research divided into 5 stages, i.e. propagation of R. solanacearum, virulence and hypersensitive tests of R. Solanacearum, antagonist test of B. subtilis and P. fluorescens against R. solanacearum on agar medium, antibiosis type test, research in greenhouse, and total phenol analysis. The result showed that all isolates of B. subtilis and P. fluorescens have potential to inhibite R. solanacearum by bacteriostatic antibiosis type. The total phenol level showed significant increase of phenol on tomato along with the application of isolates B. subtilis UB-ABS6, P. fluorescens UB-PF5 and P. fluorescens UB-PF6. Research in the greenhouse showed that all tomatoes, which had been given bioagent, did delay on the incubation than the control. Isolates of B. subtilis UB-ABS2, B. subtilis UB-ABS6, P. fluorescens UB-PF5, and P. fluorescens UB-PF6 had significantly inhibited the bacterial wilt disease 50%, 30%, 60%, and 60%, respectively. Therefore, B. subtilis and P. fluorescens can be used to control bacterial wilt diseases on tomato caused by Ralstonia solanacearum.

scholarly journals EFEKTIVITAS Pseudomonas fluorescens TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) PADA TANAMAN KACANG TANAH

2009 ◽  
Vol 9 (2) ◽  
pp. 174-180
Author(s):  
Yadi Suryadi
Keyword(s):  
Pseudomonas Fluorescens ◽  
Plant Height ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Bacterial Wilt Disease ◽  
Wilt Incidence

Effectiveness of Pseudomonas fluorescens against groundnut bacterial wilt (Ralstonia solanacearum). The experiment was conducted to study effectiveness of Pseudomonas fluorescens (PF) soil drenching application against groundnut bacterial wilt disease (Ralstonia solanacearum) suppression. Results showed that PF applications gave significant effect to reduce wilt incidence. The lowest wilt incidence (7.17%) was obtained by three times periodically soil drenching of the growing plant (1 day before planting, 15 days after planting and 30 days after planting) with PF (107 cfu/ml) suspensions. PF applications showed no significant effect on plant height which ranging from 13,07 cm - 15,60 cm.  In addition, PF applications showed wet  root and pod weight ranging from  0,35 g – 0,83 g and  7,13 g – 9,13 g, respectively which significantly different compared with untreated PF application.

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