Characterization of Recombinant Human Erythropoietin Isoforms by Capillary Isoelectric Focusing with Hydroxypropyl Cellulose-Coated Capillaries

Recombinant human erythropoietin (rhEPO) is one of the most important biopharmaceuticals worldwide, with global sales expected to reach US$11.9 billion in 2020.

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Rapid and high-resolution glycoform profiling of recombinant human erythropoietin by capillary isoelectric focusing with whole column imaging detection

Journal of Chromatography A ◽

10.1016/j.chroma.2008.03.001 ◽

2008 ◽

Vol 1190 (1-2) ◽

pp. 372-376 ◽

Cited By ~ 37

Author(s):

Peng Dou ◽

Zhen Liu ◽

Jiangang He ◽

Jing-Juan Xu ◽

Hong-Yuan Chen

Keyword(s):

High Resolution ◽

Isoelectric Focusing ◽

Recombinant Human Erythropoietin ◽

Capillary Isoelectric Focusing ◽

Human Erythropoietin ◽

Imaging Detection

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Preparation and characterization of 96-well microplates coated with molecularly imprinted polymer for determination and biosimilarity assessment of recombinant human erythropoietin

Journal of Chromatography A ◽

10.1016/j.chroma.2021.462012 ◽

2021 ◽

Vol 1641 ◽

pp. 462012

Author(s):

May A. Abd El-Aal ◽

Medhat A. Al-Ghobashy ◽

Yasser S. El-Saharty

Keyword(s):

Molecularly Imprinted Polymer ◽

Recombinant Human Erythropoietin ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Human Erythropoietin

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Capillary isoelectric focusing (CIEF) for the characterization of humic substances

Water Research ◽

10.1016/s0043-1354(97)00021-3 ◽

1997 ◽

Vol 31 (8) ◽

pp. 2037-2049 ◽

Cited By ~ 17

Author(s):

Ph. Schmitt ◽

A.W. Garrison ◽

D. Freitag ◽

A. Kettrup

Keyword(s):

Humic Substances ◽

Isoelectric Focusing ◽

Capillary Isoelectric Focusing

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Factors Affecting the Performance of Capillary Isoelectric Focusing in Dynamically Coated Capillaries Using Polyethylene Oxide Polymer

Analytical Letters ◽

10.1080/00032710802162319 ◽

2008 ◽

Vol 41 (10) ◽

pp. 1914-1931 ◽

Cited By ~ 8

Author(s):

Medhat A. Al-Ghobashy ◽

Martin A. K. Williams ◽

David R. K. Harding

Keyword(s):

Isoelectric Focusing ◽

Polyethylene Oxide ◽

Capillary Isoelectric Focusing ◽

Factors Affecting ◽

Coated Capillaries

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An improved capillary isoelectric focusing-mass spectrometry method for high-resolution characterization of monoclonal antibody charge variants

Analytical Methods ◽

10.1039/d1ay01556g ◽

2022 ◽

Author(s):

Tian Xu ◽

Linjie Han ◽

Alayna M. George Thompson ◽

Liangliang Sun

Keyword(s):

Mass Spectrometry ◽

Monoclonal Antibody ◽

High Resolution ◽

Isoelectric Focusing ◽

Capillary Isoelectric Focusing ◽

Spectrometry Method ◽

Mass Spectrometry Method ◽

Charge Variants

Routine and high-resolution characterization of monoclonal antibody (mAb) charge variants is vital for controlling mAb quality as therapeutics. Capillary isoelectric focusing-mass spectrometry (cIEF-MS) has emerged as a powerful tool for...

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