Exploitation of pentatomids by native egg parasitoids in the native and introduced ranges of Halyomorpha halys: a molecular approach using sentinel egg masses

2018 ◽  
Vol 92 (2) ◽  
pp. 609-619 ◽  
Author(s):  
J. K. Konopka ◽  
T. D. Gariepy ◽  
T. Haye ◽  
J. Zhang ◽  
B. D. Rubin ◽  
...  
Keyword(s):  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Molecular Approach ◽  
Egg Masses ◽  
Native And Introduced Ranges

scholarly journals Assemblage of the Egg Parasitoids of the Invasive Stink Bug Halyomorpha halys: Insights on Plant Host Associations

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 588
Author(s):  
Livia Zapponi ◽  
Marie Claude Bon ◽  
Jalal Melhem Fouani ◽  
Gianfranco Anfora ◽  
Silvia Schmidt ◽  
...  
Keyword(s):  
Biological Control ◽  
Mitochondrial Gene ◽  
Strong Support ◽  
Egg Parasitoids ◽  
Economic Losses ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Plant Host ◽  
Egg Masses ◽  
Host Associations

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive alien species and a key agricultural pest. Its native parasitoids (Trissolcus japonicus Ashmead and Tr. mitsukurii Ashmead) have been registered in several countries where H. halys brought dramatic economic losses and where biological control is considered to be the most effective long-term solution. By searching for stink bug egg masses and exposing sentinel egg masses, we monitored the distribution of native and exotic egg parasitoids in Trentino-Alto Adige (Italy), an area where both the host and parasitoids are in expansion. We recorded ten pentatomids, seven parasitoid species, with the first report of Tr. japonicus in this area and a hyperparasitoid. In the assemblage, Anastatus bifasciatus (Geoffroy) and Tr. mitsukurii were the dominant parasitoids, with a different distribution in terms of context and host plants. Sycamore was the host plant where the highest number of naturally laid parasitized egg masses (26%) were recorded. Trissolcus mitsukurii showed the highest parasitism rate, and was often found in apple orchards. The emergence of exotic parasitoids showed a temporal delay compared to native ones. Sequence analysis of 823 bp of the CO1 mitochondrial gene revealed that the recovered Tr. japonicus and Tr. mitsukurii harbored one single haplotype each. These haplotypes were previously found in 2018 in Northern Italy. While sentinel egg masses proved to be very effective in tracking the arrival of exotic Trissolcus species, the collection of stink bug egg masses provided fundamental data on the plant host species. The results lend strong support to the adaptation of exotic Trissolcus species to the environmental conditions of the range of introduction, providing new information on plant host-associations, fundamental for the development of biological control programs.

scholarly journals Hyperparasitism of Acroclisoides sinicus (Huang and Liao) (Hymenoptera: Pteromalidae) on Two Biological Control Agents of Halyomorpha halys

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 617
Author(s):  
Alberto Mele ◽  
Davide Scaccini ◽  
Alberto Pozzebon
Keyword(s):  
Biological Control ◽  
Management Strategies ◽  
Parasitoid Wasp ◽  
Choice Experiments ◽  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Biological Control Agents ◽  
Egg Masses ◽  
Exploitation Rate ◽  
The Impact

Halyomorpha halys (Stål) is an invasive Asian pest that causes severe crop losses on various crops. Nowadays, management strategies against this pest mainly rely on pesticide use, but biological control with egg parasitoids is considered the most promising long-term and sustainable solution. Trissolcus japonicus (Ashmead) and Trissolcus mitsukurii (Ashmead) are Asian egg parasitoids already present in Europe and are the most effective biological control agents of H. halys. Therefore, these two species are considered for biological control programs in Europe and other parts of the world. Acroclisoides sinicus (Huang and Liao) is a pteromalid parasitoid wasp that frequently emerged from H. halys egg masses collected in northern Italy. This species has been hypothesized to be a hyperparasitoid of Trissolcus spp. parasitoids. This study was carried out under laboratory conditions where A. sinicus was tested in no-choice and two-choice experiments to assess the host preference between T. japonicus and T. mitsukurii. Olfactory responses of A. sinicus from volatiles emitted from different potential hosts were also tested. In all trials, A. sinicus showed a clear preference for parasitizing H. halys eggs previously parasitized by T. mitsukurii compared to T. japonicus. In no-choice experiments, the impact of the hyperparasitoid on T. japonicus was low, showing an exploitation rate of 4.0%, while up to a 96.2% exploitation rate was observed on T. mitsukurii. Acroclisoides sinicus was also attracted by volatiles emitted by egg masses parasitized by T. mitsukurii, while no response was observed to egg masses parasitized by T. japonicus or not parasitized. Therefore, according to the results obtained here, A. sinicus could limit the population development of T. mitsukurii, while lesser effects are expected on T. japonicus.

scholarly journals Surveys in northern Utah for egg parasitoids of Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) detect Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae)

2020 ◽  
Vol 8 ◽  
Author(s):  
Mark Holthouse ◽  
Zachary Schumm ◽  
Elijah Talamas ◽  
Lori Spears ◽  
Diane Alston
Keyword(s):  
Biological Control ◽  
North America ◽  
Native Species ◽  
Insect Pest ◽  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Wasp Species ◽  
Egg Masses ◽  
Trissolcus Japonicus ◽  
Northern Utah

The highly polyphagous and invasive brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), has become a significant insect pest in North America since its detection in 1996. It was first documented in northern Utah in 2012 and reports of urban nuisance problems and plant damage have since increased. Biological control is the preferred solution to managing H. halys in North America and other invaded regions due to its alignment with integrated pest management and sustainable practices. Native and non-native biological control agents, namely parasitoid wasps, have been assessed for efficacy. Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae) is an effective egg parasitoid of H. halys in its native range of southeast Asia and has recently been documented parasitising H. halys eggs in North America and Europe. Field surveys for native and exotic egg parasitoids using wild (in situ) and lab-reared H. halys egg masses were conducted in suburban and agricultural sites in northern Utah from June to September 2017–2019. Seven native wasp species in the families Eupelmidae and Scelionidae were discovered guarding H. halys eggs and adult wasps from five of these species completed emergence. Native species had low mean rates of adult emergence from wild (0.5–3.7%) and lab-reared (0–0.4%) egg masses. In 2019, an adventive population of T. japonicus was discovered for the first time in Utah, emerging from 21 of the 106 wild H. halys egg masses found that year, and none from lab-reared eggs. All T. japonicus emerged from egg masses collected on Catalpa speciosa (Warder). Our results support other studies that have observed biological control of H. halys from T. japonicus and improved parasitoid wasp detection with wild as compared to lab-reared H. halys egg masses.

scholarly journals First Report of Native Parasitoids of Halyomorpha halys (Hemiptera: Pentatomidae) in Greece

Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 984
Author(s):  
Stefanos S. Andreadis ◽  
Nikoloz E. Gogolashvili ◽  
Georgios T. Fifis ◽  
Emmanouel I. Navrozidis ◽  
Thomas Thomidis
Keyword(s):  
Biological Control ◽  
Crop Production ◽  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Green Beans ◽  
Northern Greece ◽  
Egg Masses ◽  
Control Programs ◽  
Native Parasitoids ◽  
Wide Range

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an endemic species of East Asia; it was introduced into Europe in 2007. It has a wide range of hosts as it feeds on over 170 host plant species and significantly impacts crop production. In Greece, H. halys causes significant losses in the production of kiwi, peaches, and green beans; thus, control of this species (including biological control) is essential. Here, we focus on the potential impact of native natural enemies of H. halys in Greece. From June to October 2020, we sampled naturally field-laid H. halys egg masses to recover native parasitoids. A total of 20 egg masses of H. halys were collected from infested fields from different locations in northern Greece. Out of 529 eggs, 45 parasitoids managed to hatch successfully. The overall parasitism rate was 8.5%. We found two species of Hymenopteran egg parasitoids attacking H. halys eggs—Anastatus bifasciatus (Geoffrey) (Hymenoptera: Eupelmidae) and Ooencyrtus telenomicida (Vassiliev) (Hymenoptera: Encyrtidae), with the former comprising 58% of all parasitoids that were recovered. These results contribute to the knowledge about the natural enemy community that attacks H. halys in Greece, and the use of these native egg parasitoids in biological control programs may be a viable H. halys management strategy.

scholarly journals Rearing Trissolcus japonicus and Trissolcus mitsukurii for Biological Control of Halyomorpha halys

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 787
Author(s):  
Giuseppino Sabbatini-Peverieri ◽  
Christine Dieckhoff ◽  
Lucrezia Giovannini ◽  
Leonardo Marianelli ◽  
Pio Federico Roversi ◽  
...  
Keyword(s):  
Biological Control ◽  
Control Method ◽  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Progeny Production ◽  
Asian Origin ◽  
Frequent Use ◽  
Trissolcus Japonicus ◽  
Cumulative Curves

Halyomorpha halys is a severe agricultural pest of Asian origin that has invaded many countries throughout the world. Pesticides are currently the favored control methods, but as a consequence of their frequent use, often disrupt Integrated Pest Management. Biological control with egg parasitoids is seen as the most promising control method over the long-term. Knowledge of the reproductive biology under laboratory conditions of the most effective candidates (Trissolcus japonicus and Trissolcus mitsukurii) for optimizing production for field releases is strongly needed. Rearing of these egg parasitoids was tested by offering three different host supply regimes using new emerged females and aged, host-deprived females in different combinations. Results showed a mean progeny per female ranging from 80 to 85 specimens for T. japonicus and from 63 to 83 for T. mitsukurii. Sex ratios were strongly female biased in all combinations and emergence rates exceeded 94% overall. Cumulative curves showed that longer parasitization periods beyond 10–14 days (under the adopted rearing regimes) will not lead to a significantly increase in progeny production. However, ageing females accumulate eggs in their ovaries that can be quickly laid if a sufficient number of host eggs are supplied, thus optimizing host resources. Our data showed that offering H. halys egg masses to host-deprived female Trissolcus once a week for three weeks allowed its eggs to accumulate in the ovary, providing the greatest number of offspring within a three week span.

scholarly journals Biological control of sentinel egg masses of the exotic invasive stink bug Halyomorpha halys (Stål) in Mid-Atlantic USA ornamental landscapes

2016 ◽  
Vol 103 ◽  
pp. 11-20 ◽  
Author(s):  
Mary L. Cornelius ◽  
Christine Dieckhoff ◽  
Kim A. Hoelmer ◽  
Richard T. Olsen ◽  
Donald C. Weber ◽  
...  
Keyword(s):  
Biological Control ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Egg Masses ◽  
Exotic Invasive

Native egg parasitoids recorded from the invasive Halyomorpha halys successfully exploit volatiles emitted by the plant–herbivore complex

2017 ◽  
Vol 90 (4) ◽  
pp. 1087-1095 ◽  
Author(s):  
Gabriele Rondoni ◽  
Valeria Bertoldi ◽  
Robert Malek ◽  
Maria Cristina Foti ◽  
Ezio Peri ◽  
...  
Keyword(s):  
Egg Parasitoids ◽  
Halyomorpha Halys ◽  
Plant Herbivore

scholarly journals An effective cold storage method for stockpiling Halyomorpha halys eggs for field surveys and laboratory rearing of Trissolcus japonicus

2020 ◽  
Author(s):  
Warren H. L. Wong ◽  
Matt A. Walz ◽  
Angela B. Oscienny ◽  
Jade L. Sherwood ◽  
Paul K. Abram
Keyword(s):  
Current Method ◽  
Field Studies ◽  
Egg Parasitoid ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Time Activity ◽  
Egg Masses ◽  
Fitness Consequences ◽  
Laboratory Colony ◽  
Trissolcus Japonicus

AbstractAn effective stockpiling method for egg masses of the invasive brown marmorated stink bug (Halyomorpha halys [Stål]; Hemiptera: Pentatomidae) would be useful for rearing and field studies of its egg parasitoid Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae). The current method of stockpiling H. halys egg masses at sub-zero temperatures has lethal and sublethal fitness consequences for T. japonicus. We show that parasitoid emergence from egg masses refrigerated at 8°C for up to two months before parasitism is higher than from frozen egg masses and usually has minimal or no sublethal fitness effects (sex ratio, development time, activity, fecundity, longevity, and weight) on emerging T. japonicus. Only after two months of host egg refrigeration did the emergence of T. japonicus begin to decrease significantly (by 9.6% relative to untreated viable egg masses), whereas egg masses previously frozen at -80°C had a 58.8% reduction in parasitoid emergence after 14 days of storage. Refrigerated egg masses that were subsequently exposed to average field temperatures (warm: 22.9°C; cool: 13.2°C) were still suitable for T. japonicus parasitism after 7 days, while viable egg masses exposed to warm temperatures for 7 days before parasitism had 24.1% lower parasitoid emergence. Our results demonstrate that refrigeration at 8°C, while resulting in complete mortality of H. halys embryos after 10 days, are more suitable for T. japonicus parasitism than those stored at sub-zero temperatures. The quantity and quality of H. halys eggs that can be stockpiled with this method could facilitate T. japonicus laboratory colony maintenance, field monitoring, and releases.

scholarly journals Integrating Trissolcus japonicus (Ashmead, 1904) (Hymenoptera: Scelionidae) into Management Programs for Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae) in Apple Orchards: Impact of Insecticide Applications and Spray Patterns

Insects ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 833
Author(s):  
Dalton C. Ludwick ◽  
Jessica Patterson ◽  
Layne B. Leake ◽  
Lee Carper ◽  
Tracy C. Leskey
Keyword(s):  
The United States ◽  
Post Treatment ◽  
Egg Mass ◽  
Halyomorpha Halys ◽  
Apple Orchards ◽  
Specialty Crops ◽  
Egg Masses ◽  
Trissolcus Japonicus ◽  
Application Techniques ◽  
Pre Treatment

Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae) is an invasive species in the United States, where it has caused significant damage to specialty crops, including apples. While integrated pest management techniques have been developed for H. halys in apple, including spray application techniques, it is unknown how these techniques affect foraging, adventive Trissolcus japonicus (Ashmead, 1904) (Hymenoptera: Scelionidae), and its offspring. In this study, egg masses (unparasitized and 2 and 7 day parasitized pre-treatment) were placed in apple orchards in treated and untreated locations that received full block insecticide applications or reduced application techniques, including border row or alternate row middle applications. Bifenthrin, thiamethoxam + λ-cyhalothrin, clothianidin, and methomyl were evaluated. Egg masses were retrieved 24 h after spray applications. For 2 and 7 day parasitized pre-treatment, adult T. japonicus emergence was recorded from each egg mass. For unparasitized egg masses, T. japonicus females were given 24 h to forage and oviposit on post-treatment egg masses with female survivorship, and adult emergence from egg masses was recorded. Female survivorship was significantly lower on post-treatment egg masses retrieved from areas receiving bifenthrin applications. Emergence from post-treatment egg masses was affected by thiamethoxam + λ-cyhalothrin, bifenthrin, and methomyl in some treated areas, whereas less impact was observed on 2 and 7 day pre-treatment parasitized egg masses in general. These data provide further insights into H. halys management and the potential impact of T. japonicus in sprayed orchard agroecosystems.

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