scholarly journals Biological control of sentinel egg masses of the exotic invasive stink bug Halyomorpha halys (Stål) in Mid-Atlantic USA ornamental landscapes

2016 ◽  
Vol 103 ◽  
pp. 11-20 ◽  
Author(s):  
Mary L. Cornelius ◽  
Christine Dieckhoff ◽  
Kim A. Hoelmer ◽  
Richard T. Olsen ◽  
Donald C. Weber ◽  
...  
Keyword(s):  
Biological Control ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Egg Masses ◽  
Exotic Invasive

scholarly journals Assemblage of the Egg Parasitoids of the Invasive Stink Bug Halyomorpha halys: Insights on Plant Host Associations

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 588
Author(s):  
Livia Zapponi ◽  
Marie Claude Bon ◽  
Jalal Melhem Fouani ◽  
Gianfranco Anfora ◽  
Silvia Schmidt ◽  
...  
Keyword(s):  
Biological Control ◽  
Mitochondrial Gene ◽  
Strong Support ◽  
Egg Parasitoids ◽  
Economic Losses ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Plant Host ◽  
Egg Masses ◽  
Host Associations

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive alien species and a key agricultural pest. Its native parasitoids (Trissolcus japonicus Ashmead and Tr. mitsukurii Ashmead) have been registered in several countries where H. halys brought dramatic economic losses and where biological control is considered to be the most effective long-term solution. By searching for stink bug egg masses and exposing sentinel egg masses, we monitored the distribution of native and exotic egg parasitoids in Trentino-Alto Adige (Italy), an area where both the host and parasitoids are in expansion. We recorded ten pentatomids, seven parasitoid species, with the first report of Tr. japonicus in this area and a hyperparasitoid. In the assemblage, Anastatus bifasciatus (Geoffroy) and Tr. mitsukurii were the dominant parasitoids, with a different distribution in terms of context and host plants. Sycamore was the host plant where the highest number of naturally laid parasitized egg masses (26%) were recorded. Trissolcus mitsukurii showed the highest parasitism rate, and was often found in apple orchards. The emergence of exotic parasitoids showed a temporal delay compared to native ones. Sequence analysis of 823 bp of the CO1 mitochondrial gene revealed that the recovered Tr. japonicus and Tr. mitsukurii harbored one single haplotype each. These haplotypes were previously found in 2018 in Northern Italy. While sentinel egg masses proved to be very effective in tracking the arrival of exotic Trissolcus species, the collection of stink bug egg masses provided fundamental data on the plant host species. The results lend strong support to the adaptation of exotic Trissolcus species to the environmental conditions of the range of introduction, providing new information on plant host-associations, fundamental for the development of biological control programs.

scholarly journals An effective cold storage method for stockpiling Halyomorpha halys eggs for field surveys and laboratory rearing of Trissolcus japonicus

2020 ◽  
Author(s):  
Warren H. L. Wong ◽  
Matt A. Walz ◽  
Angela B. Oscienny ◽  
Jade L. Sherwood ◽  
Paul K. Abram
Keyword(s):  
Current Method ◽  
Field Studies ◽  
Egg Parasitoid ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Time Activity ◽  
Egg Masses ◽  
Fitness Consequences ◽  
Laboratory Colony ◽  
Trissolcus Japonicus

AbstractAn effective stockpiling method for egg masses of the invasive brown marmorated stink bug (Halyomorpha halys [Stål]; Hemiptera: Pentatomidae) would be useful for rearing and field studies of its egg parasitoid Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae). The current method of stockpiling H. halys egg masses at sub-zero temperatures has lethal and sublethal fitness consequences for T. japonicus. We show that parasitoid emergence from egg masses refrigerated at 8°C for up to two months before parasitism is higher than from frozen egg masses and usually has minimal or no sublethal fitness effects (sex ratio, development time, activity, fecundity, longevity, and weight) on emerging T. japonicus. Only after two months of host egg refrigeration did the emergence of T. japonicus begin to decrease significantly (by 9.6% relative to untreated viable egg masses), whereas egg masses previously frozen at -80°C had a 58.8% reduction in parasitoid emergence after 14 days of storage. Refrigerated egg masses that were subsequently exposed to average field temperatures (warm: 22.9°C; cool: 13.2°C) were still suitable for T. japonicus parasitism after 7 days, while viable egg masses exposed to warm temperatures for 7 days before parasitism had 24.1% lower parasitoid emergence. Our results demonstrate that refrigeration at 8°C, while resulting in complete mortality of H. halys embryos after 10 days, are more suitable for T. japonicus parasitism than those stored at sub-zero temperatures. The quantity and quality of H. halys eggs that can be stockpiled with this method could facilitate T. japonicus laboratory colony maintenance, field monitoring, and releases.

scholarly journals Testing local isolates of entomopathogenic microorganisms against Brown Marmorated Stink Bug Halyomorpha halys in Georgia

2020 ◽  
Vol 18 ◽  
pp. 00006 ◽  
Author(s):  
Medea Burjanadze ◽  
Natalia Kharabadze ◽  
Nona Сhkhidze
Keyword(s):  
Beauveria Bassiana ◽  
Black Sea ◽  
Entomopathogenic Fungus ◽  
Local Strain ◽  
High Mountain ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Disease Symptoms ◽  
Exotic Invasive

The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) (BMSB) is an exotic invasive insect which has spread extensively and established in new area of Black sea regions of Georgia. During 2017-2019, infected adults with fungal disease symptoms were found in population of H. halys from different regions of Western Georgia. Three isolates of entomopathogenic fungus Beauveria bassiana sensu lato and one of Isaria cf fumosorosea were recovered. Mycoinsecticide Bover-Ge, based on a local strain of B. bassiana-024 (isolated from soil high mountain of Caucasus Range, provide molecular identification in CABI-UK, gave a unique cultural number - IMI # 501797 and keep in CABI Genetic Recourse Collection) two concentrations (1×107 and 1×108 conidia/ml) was tested on the adults of H. halys in laboratory and its efficiency reached 72.0-90.5%. Under laboratory conditions, local isolates of nematodes Heterorhabditis bacteriophora and Steinernema borjomiensis were tested on H. halys adults. At three increasing concentrations (200, 500 and 1000 IJs/mL), H. bacteriophora caused bug mortality at the rates of 33-47%. As for Steinernema borjomiensis, the respective values were 13-33%.

Physiological Host Range of Trissolcus Mitsukurii, A Candidate Biological Control Agent of Halyomorpha Halys

2021 ◽  
Author(s):  
Lucrezia Giovannini ◽  
Giuseppino Sabbatini-Peverieri ◽  
Leonardo Marianelli ◽  
Gabriele Rondoni ◽  
Eric Conti ◽  
...  
Keyword(s):  
Biological Control ◽  
Host Range ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Classical Biological Control ◽  
Egg Parasitoid ◽  
Control Agent ◽  
Egg Load ◽  
Halyomorpha Halys ◽  
Stink Bug

Abstract The invasive stink bug Halyomorpha halys is a severe agricultural pest of worldwide importance, and chemical insecticides are largely sprayed for the control of its populations. Negative impacts and several failures in chemical pest management led to consider classical biological control as one of the most promising methods in a long-term perspective. The Asian egg parasitoid Trissolcus japonicus is the main candidate biocontrol agent of H. halys, but more recently a second Asian egg parasitoid, Trissolcus mitsukurii, is getting attention after adventive populations were found on H. halys egg masses in Europe. Before recommending the use of T. mitsukurii for biological control of H. halys, a risk analysis is necessary and therefore here we present the first study on the fundamental physiological host range of this parasitoid in Europe. Tests conducted with T. mitsukurii on different hemipterans, using three different experimental designs, revealed a broad physiological host range, comparable with the host range displayed by T. japonicus under similar laboratory conditions. Specifically, in addition to its coevolved host H. halys, T. mitsukurii successfully parasitized the majority of tested pentatomid species and one scutellerid, although with highly variable emergence rates. Host egg sizes positively affected parasitoid size and female egg load. Further studies, testing more complex systems that involve olfactory cues from host and host plants, will simulate different aspects of the parasitoid host location behavior under field conditions, allowing in-depth evaluation of the possible risks associated with the use of T. mitsukurii as a biocontrol agent of H. halys.

Effects of Aggregation Lure and Tree Species on Halyomorpha halys (Hemiptera: Pentatomidae) Seasonal Oviposition

2019 ◽  
Author(s):  
Adam Formella ◽  
Seth J Dorman ◽  
Sally V Taylor ◽  
Thomas P Kuhar
Keyword(s):  
Host Density ◽  
Egg Parasitoid ◽  
Egg Laying ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Egg Masses ◽  
Cercis Canadensis ◽  
Augmentative Releases ◽  
Ornamental Trees ◽  
Tree Fruits

Abstract The brown marmorated stink bug, Halyomorpha halys (Stål), is a polyphagous pest that feeds on a wide variety of agricultural commodities including tree fruits, berries, vegetables, field crops, and ornamental trees and shrubs. Accurate knowledge of where H. halys lays eggs is critical to optimize the potential release of Trissolcus japonicus (Ashmead), a scelionid egg parasitoid native to the same host region as H. halys. Ideally, parasitoids should be released in and around areas with high host density. In southwestern Virginia in 2017 and 2018, we searched trees for egg masses in an urban environment and nonmanaged wooded border environment. We also evaluated the effects of a commercial aggregation lure on the number of eggs being deposited. This aggregation lure, when combined with methyl (E,E,Z)-2,4,6-decatrienoate (MDT), has been shown to attract both adult and nymph H. halys and its effects on egg laying were not known. Results of this study showed no difference between the number of eggs laid on trees with and without lures. Catalpa trees, Catalpa bignonioides Walter, had the most egg masses throughout the course of the study; however, the redbud, Cercis canadensis L., had similar numbers in the late July and August. There was an overall trend with more eggs masses found on trees with fruiting structures present. This information can provide insight on where and when to make augmentative releases of egg parasitoids for H. halys.

scholarly journals Potentials of some entomopathogens against the brown marmorated stink bug, Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae)

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Elif Tozlu ◽  
Islam Saruhan ◽  
Göksel Tozlu ◽  
Recep Kotan ◽  
Fatih Dadaşoğlu ◽  
...  
Keyword(s):  
Biological Control ◽  
Chemical Control ◽  
Mortality Rates ◽  
Fungal Isolate ◽  
Economic Losses ◽  
Halyomorpha Halys ◽  
Pest Species ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Bacterial Isolates

AbstractThe brown marmorated stink bug, Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae), is an invasive harmful pest species due to its economic losses. Its wide host range and continuous movement make its control difficult with insecticides. Biological control has recently gained importance due to the negative aspects of chemical control. The study evaluated the biological control tools by testing the entomopathogens against the pest by 11 bacteria strains and 1 fungal isolate. Brevibacillus, Bacillus, Pantoea, Vibrio, Pseudomonas, and Beauveria were tested against the nymphs of H. halys under controlled conditions. All applied entomopathogens had potentials for controlling H. halys. Mortality rates of 75 and 100% were obtained by the bacteria strains and 76.19% by the fungus, B. bassiana. Successfully reaching a 100% control rate, the bacterial isolates of the Bacillus cereus GC subgroup B and Pantoea agglomerans GC subgroup were recorded to have a greater potential than the others.

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