scholarly journals The Effects of Some Antioxidants on the Growth of Excised Date Palm (Phoenix dactylifera L.) Roots Cultured in Vitro

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 875B-875
Author(s):  
Yousef I. Dlaigan ◽  
A.E. Said ◽  
M.A. El-Hamady
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Date Palm ◽  
Root Culture ◽  
Phoenix Dactylifera ◽  
Sodium Metabisulfite ◽  
Phoenix Dactylifera L ◽  
Significant Difference

The objective of this investigation was to study the effects of some antioxidants on the growth and elongation of in vitro-cultured date palm roots. Several trials were conducted to determine the antioxidant and the concentration of antioxidants used in date palm tissue culture. Three types of antioxidants were tested—activated charcoal, polyvinylpyrrolidone (PVP), and sodium metabisulfite (NA2S2O5)—in various concentrations, singly, or in combinations. The medium used for root culture consisted of MS salts mixture, 1/2 modified White's organics, 60% sucrose, 0.004% inositol, 240 mg adenine sulfate/liter, 1.0 mg 2,4-D/liter, and 2.0 mg kinetin/liter. pH was adjusted at 5.7 ± 0.1. Concentrations of activated charcoal tested (in g–liter–1) were: 0.0, 1, 2, 3, 4, 6, 8, 10, and those of PVP (in g–liter–1) were: 0.0, 5, 10, 20, 40 (with or without 4 g activated charcoal/liter). NA2S2O5 concentrations (in mM) tested were: 0.0, 0.2, 0.4, 0.8, 1.6 (with 4 g activated charcoal/liter). The addition of activated charcoal to the culture medium of date palm roots is of vital importance. Four grams per liter gave the best growth and elongation, and there was no significant difference between it and 10 g–liter–1. No growth or elongation occurred in the control. PVP, however, significantly inhibited growth and elongation of cultured roots at all concentrations tested. NA2S2O5, however, stimulated growth and elongation at 0.2 mM. Higher concentrations inhibited growth and elongation.

scholarly journals The Influence of the Physical State of the Nutrient Media on the Growth of Excised Date Palm (Phoenix dactylifera L.) Roots

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 875C-875
Author(s):  
Yousef I. Dlaigan ◽  
A.E. Said ◽  
M.A. El-Hamady
Keyword(s):  
Activated Charcoal ◽  
Nutrient Medium ◽  
Physical State ◽  
Date Palm ◽  
Root Culture ◽  
Phoenix Dactylifera ◽  
Liquid Media ◽  
Nutrient Media ◽  
Phoenix Dactylifera L ◽  
Significant Difference

The effects of the physical state of nutrient media on the growth and elongation of excised date palm roots were investigated. Roots were cultured in a nutrient medium containing MS salts mixture, 1/2 modified White's organics, and (in mg–liter–1): NaH2PO4–H2O, 170; sucrose, 60,000; inositol, 40; adenine sulfate, 240; activated charcoal, 4000; 2,4-D, 1; kinetin, 2. pH was adjusted at 5.7 ± 0.1. Both agar and Gelrite were singly used as solidifying agents. Liquid media were either stationary or rotated on gyratory shakers at 70 to 80 rpm. The effects of incubation of cultured roots under light or dark conditions were also studied. Media pH and its effects on growth and elongation of cultured roots were tested at various ranges (3, 4, 5, 6, 7, 8, 9, 10). Trials were also made to determine the passage length for transfer and subculture of cultured roots to a newly prepared medium. Liquid media highly supported better growth and elongation of cultured roots compared to solidified media. There was no significant difference in growth or elongation between agar or Gelrite media. Shaking liquid medium resulted in significantly better growth and elongation compared to stationary medium. No difference was observed between dark- and light-incubated cultured roots. Cultured roots grew and elongated better at pH 7.0–8.0. No growth or elongation occurred at pH 9.0. Roots continued to grow and elongate even after 12 weeks in culture. Therefore, 10 to 12 weeks after culture was determined to be the optimum passage length for date palm root culture.

scholarly journals Effects of Polyamines And Silver Thiosulphate On Phoenix Dactylifera L. Cv. Quntar Micropropagation And Molecular Analysis.

2021 ◽  
Author(s):  
Ahmed Almayahi
Keyword(s):  
Tissue Culture ◽  
Date Palm ◽  
Genetic Fidelity ◽  
Phoenix Dactylifera ◽  
Silver Thiosulfate ◽  
Practical Applications ◽  
Root Regeneration ◽  
Phoenix Dactylifera L ◽  
Fidelity Assessment

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. The objective of the present study is to determine the effect of polyamines (putrescine "PUT" and spermidine" SPD") and silver thiosulfate (STS) on enhancing propagation of date palm cv Quntar in vitro. Media supplemented with 75 mg L-1 SPD in combination with 10 mgL-1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root regeneration and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL-1 PUT and 10 mgL-1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

scholarly journals In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ahmed Madi Waheed Al-Mayahi
Keyword(s):  
Biochemical Analysis ◽  
Culture Medium ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Tissue Cultures ◽  
In Vitro Plant Regeneration ◽  
Regeneration System ◽  
Iron Chelate ◽  
Phoenix Dactylifera L

Abstract Background Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. Results The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe), compared with other treatments. The results also indicate that using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L−1 (16.8 mg L−1 Fe) and 187.0 mg L−1 (11.2 mg L−1Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) as Fe source. Conclusion FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.

scholarly journals In vitro response of date palm (Phoenix dactylifera L.) inflorescence explants to high 2iP and 2,4-D concentrations

2020 ◽  
pp. 831-835
Author(s):  
Juliana Martins Ribeiro ◽  
Silvio Lopes Teixeira ◽  
Joselita Cardoso de Souza ◽  
Brenda Lima Ribeiro ◽  
Antônio Bruno Nunes Oliveira ◽  
...  
Keyword(s):  
Culture Medium ◽  
Date Palm ◽  
Root Formation ◽  
Phoenix Dactylifera ◽  
Culture Technique ◽  
Phoenix Dactylifera L ◽  
Inflorescence Explants ◽  
In Vitro Response ◽  
Floral Bud

One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, explants did not have swelling, callus or root formation. The treatment containing 150 mg/L 2,4-D in the presence of 1.5 mg/L 2iP initiated explant swelling, and treatments with either 100 mg/L or 150 mg/L 2,4-D, combined with 3.0 mg/L 2iP, were also efficient in stimulating in vitro swelling of inflorescence buds. Rhizogenesis was induced at the highest concentrations of 2,4-D (100 and 150 mg/L), combined with 4.5 mg/L 2iP, and was visually more evident in the treatment containing 150 mg/L 2,4-D + 4.5 mg/L 2iP. These results suggest that even higher concentrations of these two reagents might be efficient in the micropropagation of new existing date palm genotypes in the Submedium São Francisco River Valley.

Optimizing Tissue Culture Protocol for In Vitro Shoot and Root Development and Acclimatization of Date Palm (Phoenix dactylifera L.) Plantlets

2022 ◽  
Author(s):  
Najamuddin Solangi ◽  
Mushtaque Ahmed Jatoi ◽  
Ghulam Sarwar Markhand ◽  
Adel Ahmed Abul-Soad ◽  
Muhammad Aslam Solangi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Root Development ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L ◽  
Culture Protocol

scholarly journals The Effect of Thidiazuron on Callus and in vitro Shoots Development of Date Palm (Phoenix dactylifera L.) cv. Barhee

2019 ◽  
Vol 32 ◽  
pp. 258-265 ◽  
Author(s):  
Ahmed Z. Al-Asadi ◽  
Aqeel H. Abdulwahid ◽  
Ahmed M.W. Al-Mayahi
Keyword(s):  
Tissue Culture ◽  
Date Palm ◽  
Fruit Trees ◽  
Phoenix Dactylifera ◽  
Optimal Concentration ◽  
Working Paper ◽  
Phoenix Dactylifera L ◽  
In Vitro Shoots ◽  
Southern Iraq

Date palm (Phoenix dactylifera L.) is one of the most important fruit trees spread in southern Iraq, but because of many circumstances decreased their numbers significantly, the propagation of date palm by tissue culture was the best solution for re-preparation of the problem as it was previously, because propagation is accelerated. Summarizes the working paper in an attempt to find the optimal concentration of thidiazuran (TDZ) (0.0 , 0.5 and 1.0) mgl-1, in production of callus and regeneration of shoots, at different times of initial and callus culturing in medium budding. The results showed that the concentration of (0.5) mgl-1 was optimal in all traits under study.

scholarly journals 2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

2021 ◽  
Author(s):  
Emna Baklouti ◽  
Thierry Beulé ◽  
Amèni Nasri ◽  
Amal Ben Romdhane ◽  
Riadh Drira ◽  
...  
Keyword(s):  
Date Palm ◽  
Specific Activity ◽  
Phoenix Dactylifera ◽  
Proline Content ◽  
Epigenetic Changes ◽  
Negative Effects ◽  
Somaclonal Variations ◽  
Phoenix Dactylifera L ◽  
Free Media

Abstract The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L− 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g− 1 to 2973 nmol g− 1 FW when 40 mg L− 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA); likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8–18.93%.

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