In vitro regeneration and multiplication for mass propagation of Acacia ehrenbergiana Hayne: a potential reclaiment of denude arid lands

Radermachera xylocarpa (Roxb.) K. Schum. is a rare indigenous forest tree species which is utilized for its wood and medicinal properties. Due to its overexploitation and specific habitat requirements the species is restricted to limited areas. In vitro mass propagation of tree species faces various challenges and no such efforts have yet been taken in propagation of this useful plant using these methods. In order to overcome the hurdles and understanding an urgent need of its conservation and mass propagation present authors attempt to develop a simple effective tissue culture protocol for regeneration of R. xylocarpa. Nodal explants were cultured on MS supplemented with various concentrations of cytokinins and auxins. Among different cytokinins, maximum bud induction and proliferation was obtained in media supplemented with Kn along with IBA and for effective root induction which is tough to obtain in tree species, 100% rooting was achieved in cultures with increasing concentrations of IBA. Field survival is a major challenge with regenerated plants of forest tree species. We report here for the first time 100% survival of plants in soil by carefully standardizing the period of hardening and acclimatization procedures. A novel and effective in vitro regeneration protocol of R. xylocarpa has been successfully standardized which can be adopted for large scale propagation, reforestation and conservation of rare Radermachera xylocarpa of medicinal importance.Plant Tissue Cult. & Biotech. 23(1): 21?29, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15556

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In vitro regeneration of a common medicinal plant, Ocimum sanctum L. for mass propagation

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2015.15200 ◽

2016 ◽

Vol 15 (24) ◽

pp. 1269-1275 ◽

Cited By ~ 1

Author(s):

Abu Hena Mostofa Jamal Mohammad ◽

Hoque Sharif Imdadul ◽

Mostofa Shakil Md ◽

N M Rubaiyath Bin Rahman A ◽

Akhter Banu Nilufa ◽

...

Keyword(s):

Medicinal Plant ◽

In Vitro Regeneration ◽

Ocimum Sanctum ◽

Mass Propagation

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Effects of different media strengths and hormone concentrations on in-vitro regeneration of Vitellaria paradoxa C.F. Gaertn

Nigerian Journal of Biotechnology ◽

10.4314/njb.v37i1.16 ◽

2020 ◽

Vol 37 (1) ◽

pp. 150-158

Author(s):

J.O. Afolabi ◽

E.M. Olorode ◽

D.B. Olomola ◽

Y.O. Fasakin ◽

E.A Adekunle

Keyword(s):

In Vitro Propagation ◽

In Vitro Regeneration ◽

Deciduous Tree ◽

Shea Butter ◽

Vitellaria Paradoxa ◽

Mass Propagation ◽

Charcoal Production ◽

Nodal Cuttings ◽

Half Strength

The importance of Vitellaria paradoxa has necessitated its inclusion in the ongoing afforestation project which requires mass production of its seedlings. The shea-butter tree produces oil-rich seeds that are important to cosmetic and food and nutrition industries. It is a deciduous tree which could survive in the dry-savannah and help withstand desert encroachment. However, overexploitation of its timber for firewood and charcoal production made it vulnerable. In-vitro propagation of this species could be a viable means towards its mass propagation. Two plant-hormones: Gibberellic Acid (GA3) and Benzyl Amino Purine (BAP) were used to grow the plant in different combinations making up six treatments (A-F) with 6 replicates in each group. The combinations include 1.5/0, 1.5/1.0, 1.5/1.5, 2.0/0, 2.0/1.0, 2.0/1.5 mg/L of GA3/BAP, which were used to grow 2cm nodal cuttings of the V. paradoxa on half-strength Murashige and Skoog (MS) media. Treatment F (with 2.0/1.5 mg/L GA3/BAP) produced the highest number of leaves (2.4 ± 0.6, 3.2 ± 0.8) and longest shoot length (1.1 ± 0.3 cm, 1.4 ± 0.5 cm) at 4 and 8 Weeks After Inoculation (WAI), respectively. The results of this study showed that application of growth- enhancing hormones for the regeneration of important tree species could provide means for their mass propagation so as to meet the need for afforestation projects. Keywords: Vitellaria paradoxa, Afforestation, In-vitro propagation, Mass propagation.

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In Vitro Regeneration and Mass Propagation of Ruta graveolens L.—A Multipurpose Shrub

HortScience ◽

10.21273/hortsci.40.5.1478 ◽

2005 ◽

Vol 40 (5) ◽

pp. 1478-1480 ◽

Cited By ~ 12

Author(s):

Mohd Faisal ◽

Naseem Ahmad ◽

Mohammad Anis

Keyword(s):

High Frequency ◽

In Vitro Regeneration ◽

Nodal Segments ◽

Ms Medium ◽

Ruta Graveolens ◽

Mass Propagation ◽

Regeneration Frequency ◽

Whole Plant ◽

Half Strength

A protocol for rapid in vitro propagation of Ruta graveolens L. through high-frequency shoot induction from nodal explants was established. Proliferation of shoots from nodal segments was achieved on Murashige and Skoog medium supplemented with various concentrations of BA, Kin, IAA, and NAA, either singly or in various combinations. The highest shoot regeneration frequency (98.5%) and the highest number of shoots per explant (40.2 ± 2.8) was obtained on MS medium supplemented with 10 μm BA and 2.5 μm NAA. In vitro regenerated shoots rooted best on half-strength MS medium containing 0.5 μm IBA. Rooted shoots, following acclimatization in the greenhouse, were successfully transferred to field conditions, and 90% of plants survived. The efficient in vitro regeneration of the whole plant can be used as a fast and reliable method to transform R. graveolens genetically for its active principles.

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