Thermohalobaculum xanthum gen. nov., sp. nov., a moderately thermophilic bacterium isolated from mangrove sediment

2021 ◽  
Author(s):  
Xinli Pan ◽  
Zhe Li ◽  
Fei Li ◽  
Yuanlin Huang ◽  
Qiaozhen Wang ◽  
...  
Keyword(s):  
Thermophilic Bacterium ◽  
Mangrove Sediment ◽  
Moderately Thermophilic

scholarly journals Thermohalobaculum Xanthum Gen. Nov., Sp. Nov., A Novel Moderately Thermophilic Bacterium Isolated From Mangrove Sediment

2021 ◽  
Author(s):  
Xinli Pan ◽  
Fei Li ◽  
Zhe Li ◽  
Yuanlin Huang ◽  
Qiaozhen Wang ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Nutrient Utilization ◽  
Thermophilic Bacterium ◽  
Mangrove Sediment ◽  
Species Discrimination ◽  
Rrna Gene ◽  
Beibu Gulf ◽  
Moderately Thermophilic ◽  
The Family

Abstract A novel moderately thermophilic and halophilic bacterium, designated strain M0105T, was isolated from mangrove sediment collected in the Beibu Gulf, south China. The isolate is Gram-negative, non-motile and rod-shaped bacterium with smooth colonies of pale-yellow appearance. Growth occurs at 15-46℃ (optimum 37-40℃) and pH range of 6.0-10.0 (optimum pH 8.0-9.0). It required 1-7% NaCl (optimum 3-5%) for growth. Strain M0105T was affiliated to the family ‘Rhodobacteraceae’, sharing the highest 16S rRNA gene sequence similarity with Limibaculum halophilum CAU 1123T (96.8%). The major menaquinone Q-10 and the dominant unsaturated fatty acid (C18:1ω7) in this family were also detected in the strain M0105T. The genome sequence possesses a circular 4.1 Mb chromosome with a G+C content of 67.9%. Strain M0105T encoded many genes for cellular stress resistance and nutrient utilization, which could improve its adaptive capacity to the mangrove environment. Values of conserved proteins (POCP), average nucleotide identity (ANI), average amino acid identity (AAI) and DNA-DNA hybridization (dDDH) between the isolate and closely related species were below the proposed threshold for species discrimination. Information from phenotypic, chemotaxonomic and phylogenetic analyses proposed that strain M0105T should be assigned to a novel genus within the family ‘Rhodobacteraceae’. Thus, we suggested that the strain M0105T represents a novel species in a new genus, for which the name Thermohalobaculum xanthum gen. nov., sp. nov. is proposed. The type strain of the type species is M0105T (=BGMRC 2019T=KCTC 52118T =MCCC 1K03767T=NBRC 112057T).Six supplementary figures and two supplementary tables are available with the online version of this article.

scholarly journals Thermobacillus composti sp. nov., a moderately thermophilic bacterium isolated from a composting reactor

2007 ◽  
Vol 57 (7) ◽  
pp. 1473-1477 ◽  
Author(s):  
Keiko Watanabe ◽  
Norio Nagao ◽  
Shuich Yamamoto ◽  
Tatsuki Toda ◽  
Norio Kurosawa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Moderately Thermophilic

A Gram-negative, rod-shaped, spore-forming and moderately thermophilic bacterium, strain KWC4T, was isolated from a composting reactor. Cells of strain KWC4T were 2.0–5.0 μm long and 0.5–0.7 μm in diameter. Strain KWC4T grew aerobically at 32–61 °C, with optimal growth occurring at 50 °C. It grew at pH 5.6–10.1, with optimal growth at around pH 9.0. The optimum NaCl concentration for growth was almost 0 % (w/v), but strain KWC4T was moderately halotolerant and was able to grow at NaCl concentrations up to 4.4 % (w/v). The DNA G+C content of strain KWC4T was 60.0 mol%. The major fatty acids were iso-16 : 0 (39.0 %) and anteiso-15 : 0 (33.3 %). Based on 16S rRNA gene sequence similarity data, strain KWC4T belonged to the genus Thermobacillus and was related to Thermobacillus xylanilyticus. However, strain KWC4T had a 38 bp insertion sequence located near the 3′ end of its 16S rRNA gene that was not present in T. xylanilyticus. The 16S rRNA gene sequence similarity value between strain KWC4T and T. xylanilyticus was 95.7 %. The DNA–DNA hybridization value between strain KWC4T and T. xylanilyticus strain XET was 66 %. On the basis of phenotypic and genotypic evidence, strain KWC4T (=DSM 18247T=JCM 13945T) is the type strain of a novel species, for which the name Thermobacillus composti sp. nov. is proposed.

Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring

2018 ◽  
Vol 111 (12) ◽  
pp. 2275-2282 ◽  
Author(s):  
Inam Ullah Khan ◽  
Neeli Habib ◽  
Min Xiao ◽  
Asem Mipeshwaree Devi ◽  
Maham Habib ◽  
...  
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Moderately Thermophilic

scholarly journals Aquabacterium tepidiphilum sp. nov., a moderately thermophilic bacterium isolated from a hot spring

2019 ◽  
Vol 69 (2) ◽  
pp. 337-342 ◽  
Author(s):  
Inam Ullah Khan ◽  
Neeli Habib ◽  
Mipeshwaree Devi Asem ◽  
Nimaichand Salam ◽  
Min Xiao ◽  
...  
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Moderately Thermophilic

scholarly journals Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 849-855 ◽  
Author(s):  
Kadriye Inan ◽  
Sabriye Canakci ◽  
Ali Osman Belduz ◽  
Fikrettin Sahin
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Lipid Analysis ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Gene Sequence Analysis

Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus ; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15 : 0 (39.30 %), anteiso-C15 : 0 (26.10 %) and iso-C16 : 0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA–DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T ( = DSM 24395T = LMG 26289T).

scholarly journals Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, nitrate-reducing bacterium isolated from an Australian oil well

2004 ◽  
Vol 54 (3) ◽  
pp. 645-649 ◽  
Author(s):  
Monica Bonilla Salinas ◽  
Marie-Laure Fardeau ◽  
Jean-Luc Cayol ◽  
Laurence Casalot ◽  
Bharat K. C. Patel ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Growth Conditions ◽  
Thermophilic Bacterium ◽  
Oil Reservoir ◽  
Oil Well ◽  
Terminal Electron Acceptor ◽  
Gram Negative ◽  
Moderately Thermophilic ◽  
Bacterium Strain

A novel Gram-negative, aerobic and moderately thermophilic bacterium, strain 4BONT, was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 °C, pH 6·9 and 0·5 % NaCl. Strain 4BONT was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58·6 mol%. The closest phylogenetic relative of strain 4BONT was Hydrogenophilus thermoluteolus (similarity of 91·8 %), of the β-Proteobacteria. As strain 4BONT is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BONT (=DSM 15512T=CIP 107790T).

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