Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae cultured in recirculating aquaculture systems with and without biofloc

2018 ◽  
Vol 27 (1) ◽  
pp. 209-225 ◽  
Author(s):  
Jorge Soto-Alcalá ◽  
Píndaro Álvarez-Ruiz ◽  
J. M. Audelo-Naranjo ◽  
H. M. Esparza-Leal ◽  
I. E. Luis-Villaseñor ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Transcriptional Response ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems ◽  
Immune Related Genes

scholarly journals Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

2021 ◽  
Vol 50 ◽  
Author(s):  
Ramón Casillas-Hernández ◽  
Karla Janeth Arévalo-Sainz ◽  
María Belem Flores-Pérez ◽  
Jesus Guadalupe Garcia-Clark ◽  
Libia Zulema Rodriguez-Anaya ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Litopenaeus Vannamei ◽  
Transcriptional Response ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems ◽  
Immune Related Genes

scholarly journals Comparing RAS with and without biofloc: Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae

2020 ◽  
Vol 33 (1) ◽  
pp. 32-43
Author(s):  
Jorge Soto Alcalá ◽  
Píndaro Álvarez Ruiz ◽  
Juan M Audelo Naranjo ◽  
Héctor M Esparza Leal ◽  
Irasema E Luis Villaseñor ◽  
...  
Keyword(s):  
Immune System ◽  
Litopenaeus Vannamei ◽  
Tumor Necrosis Factor Receptor ◽  
Transcriptional Response ◽  
Early Response ◽  
Shrimp Farming ◽  
Rt Pcr ◽  
Recirculating Aquaculture ◽  
El Sistema ◽  
Immune Related Genes

Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and noBF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei. Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were upregulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response.Keywords: antimicrobial peptide; aquaculture; biofloc; gene expression; Penaeidin; RAS; shrimp; shrimp immune system.  Resumen Antecedentes: Los cultivos de camarón están evolucionando de sistemas semi-intensivos a hiper-intensivos con biofloc y con recirculación. Objetivo: Evaluar la respuesta transcripcional promovida por el biofloc en un sistema acuícola con recirculación (SAR). Métodos: Monitoreamos mediante RT-PCR cuantitativo siete genes relacionados con el sistema inmune en postlarvas de camarón cultivadas en un SAR con y sin biofloc (BF y no-BF). Además, presentamos por primera vez la secuencia de nucleótidos del factor de ribosilación 4 de ADP (LvArf4) de Litopenaeus vannamei. Resultados: Los genes penaeidina3 (Pen3), penaeidina4 (Pen4), Crustina y Toll (LvToll) se sobre-expresaron entre las 3 y 24 h en ambos sistemas, y el factor 6 asociado al factor de necrosis tumoral (TRAF6) en BF como una respuesta temprana. Con respecto a la expresión diferencial entre los tratamientos, se presentaron 13 ocurrencias. Nueve donde el BF fue mayor que sin-BF y cuatro donde el no-BF fue mayor que el BF. La expresión fue más alta en BF que en no-BF en Pen3, Crustin, LvToll, TRAF6, IMD y LvArf4. En contraste, la expresión fue mayor en no-BF en Pen3, Pen4 y TRAF6. Conclusiones: el BF modula la transcripción de los genes relacionados con la respuesta inmune en camarón como una respuesta temprana. Sin embargo, el SAR sin-BF promueve una respuesta similar.Palabras clave: aquicultura; biofloc; camarón; expresión genética; Penaeidina; péptido antimicrobiano; SAR; sistema inmune de camarón.  Resumo Antecedentes: A criação de camarões está evoluindo de sistemas semi-intensivos para hiper-intensivos como tecnologia de bioflocos e sistemas de recirculação. Objetivo: Avaliar a resposta transcricional promovida pelo biofloco em um sistema de aquicultura recirculante (SAR). Métodos: Utilizamos RT-PCR quantitativo em tempo real para monitorar sete genes-chave relacionados ao sistema imune em pós-larvas de camarão, criados em SAR com e sem bioflocos (BF e no-BF). Além disso, apresentamos pela primeira vez sequências nucleotídicas do fator de ribosilação do ADP 4 (LvArf4) de Litopenaeus vannamei. Resultados: Os resultados mostraram que o Penaeidina3 (PEN3), Penaeidina4 (Pen4), Crustina e Toll genes (LvToll) foram sobre-expressos entre 3 e 24 h em ambos os sistemas, e o Factor de Necrose do Receptor 6 associado e protuberância (TRAF6) no BF como uma resposta precoce. Com relação à expressão diferencial entre tratamentos, 13 ocorrências foram apresentadas. Nove onde o BF foi maior do que os não-BF e quatro onde o não-BF foi maior do que o BF. A expressão foi maior do que em BF não-BF em Pen3, Crustin, LvToll, TRAF6, IMD e LvArf4. Em contraste, a expressão foi mais elevada no não-BF em Pen3, Pen4 e TRAF6. Conclusões: O BF modula a transcrição de resposta imune relacionada no camarão como um genes de resposta precoce. No entanto, o SAR não BF promove uma resposta semelhante.Palavras-chave: aquicultura; biofloco; camarão; expressão genética; Penaeidina; peptídeo antimicrobiano; SAR; sistema imunológico do camarão.

scholarly journals Retrofitting Sea Cucumber Nursery Tanks to Recirculating Aquaculture Systems for Highly Intensive Litopenaeus vannamei Aquaculture

2021 ◽  
Vol 11 (20) ◽  
pp. 9478
Author(s):  
Yishuai Du ◽  
Jianping Xu ◽  
Li Zhou ◽  
Fudi Chen ◽  
Tianlong Qiu ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Sea Cucumber ◽  
Stocking Density ◽  
Ammonia Nitrogen ◽  
Average Weight ◽  
Recirculating Aquaculture ◽  
Nitrite Nitrogen ◽  
Innovative Methodology ◽  
Recirculating Aquaculture Systems ◽  
Total Ammonia

Designing good recirculating aquaculture systems (RASs) is challenging in shrimp aquaculture. In this study, two sets of RASs were constructed using sea cucumber nursery tanks for rearing Litopenaeus vannamei. Recirculating aquaculture was supported by key technologies such as sewage collection and aeration systems adapted to the rectangular tanks and technologies for the removal of sewage, shrimp shells, and dead individuals. Six-hundred and eighty-five thousand juveniles were selected for rearing in the newly constructed RASs, where the average stocking density was 1013 shrimp/m3. During the recirculating aquaculture period of 53 days, the water temperature of the tanks was 24–31 °C, the salinity was 25–32‰, the pH was 6.4–8.2, the DO was ≥ 4.9 mg/L, the concentration of total ammonia nitrogen (TAN) was maintained between 0.17 and 4.9 mg/L, the concentration of nitrite nitrogen (NO2-N) was between 0.12 and 4.7 mg/L, and the total number of Vibrio bacteria remained between 330 and 9700 cfu/mL. At the end of the experiment, the final average weight of individual shrimp was 13.43 g, and the average yield reached 12.92 kg/m3. The great improvement in growth performance marks a breakthrough in RAS technology of shrimp, and it supports the use of an innovative methodology for the retrofitting and utilization of idle sea cucumber nursery tanks.

Effects of carbon source addition on microbial community and water quality in recirculating aquaculture systems for Litopenaeus vannamei

2020 ◽  
Vol 86 (3) ◽  
pp. 507-517
Author(s):  
Zhao Chen ◽  
Zhiqiang Chang ◽  
Long Zhang ◽  
Jiajia Wang ◽  
Ling Qiao ◽  
...  
Keyword(s):  
Water Quality ◽  
Microbial Community ◽  
Carbon Source ◽  
Litopenaeus Vannamei ◽  
Recirculating Aquaculture ◽  
Recirculating Aquaculture Systems
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