Examining bivalve fecundity: oocyte viability revealed by Neutral Red vital staining

2021 ◽  
Author(s):  
Peter G. Beninger ◽  
Daphné Chérel ◽  
Lucie Kessler
Keyword(s):  
Vital Staining ◽  
Neutral Red

Interdigital tissue chondrogenesis induced by surgical removal of the ectoderm in the embryonic chick leg bud

Development ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 231-244
Author(s):  
J. M. Hurle ◽  
Y. Gañan
Keyword(s):  
Cell Death ◽  
Vital Staining ◽  
Mesenchymal Cell ◽  
Neutral Red ◽  
Surgical Removal ◽  
Experimental Conditions ◽  
Local Inhibition ◽  
Dorsal Ectoderm ◽  
Interdigital Cell Death ◽  
Proximal Zone

In the present work, we have analysed the possible involvement of ectodermal tissue in the control of interdigital mesenchymal cell death. Two types of experiments were performed in the stages previous to the onset of interdigital cell death: (i) removal of the AER of the interdigit; (ii) removal of the dorsal ectoderm of the interdigit. After the operation embryos were sacrificed at 10–12h intervals and the leg buds were studied by whole-mount cartilage staining, vital staining with neutral red and scanning electron microscopy. Between stages 27 and 30, ridge removal caused a local inhibition of the growth of the interdigit. In a high percentage of the cases, ridge removal at these stages was followed 30–40 h later by the formation of ectopic nodules of cartilage in the interdigit. The incidence of ectopic cartilage formation was maximum at stage 29 (60%). In all cases, cell death took place on schedule although the intensity and extent of necrosis appeared diminished in relation to the intensity of inhibition of interdigital growth and to the presence of interdigital cartilages. Ridge removal at stage 31 did not cause inhibition of the growth of the interdigit and ectopic chondrogenesis was only detected in 3 out of 35 operated embryos. Dorsal ectoderm removal from the proximal zone of the interdigit at stage 29 caused the chondrogenesis of the proximal interdigital mesenchyme in 6 out of 18 operated embryos. The pattern of neutral red vital staining was consistent with these results revealing a partial inhibition of interdigital cell death in the proximal zone of the interdigit. It is proposed that under the present experimental conditions the mesenchymal cells are diverted from the death programme by a primary transformation into cartilage.

VITAL STAINING PROPERTIES OF NEUTRAL RED

2009 ◽  
Vol 56 (5) ◽  
pp. 742-750 ◽  
Author(s):  
KIRSTEN MARNER ◽  
MOGENS S. NORN
Keyword(s):  
Vital Staining ◽  
Neutral Red

The Preparation of Neutral Red Iodide Suitable for Vital Staining

1927 ◽  
Vol 2 (1) ◽  
pp. 17-18 ◽  
Author(s):  
Max Phillips ◽  
Barnett Cohen
Keyword(s):  
Vital Staining ◽  
Neutral Red

A NEW METHOD FOR VITAL STAINING OF CENTRAL NERVOUS TISSUES WITH NEUTRAL RED

1971 ◽  
Vol 21 (1) ◽  
pp. 151-155
Author(s):  
Ryuei Maeda ◽  
Nobuo Ihara ◽  
Reiko Takada ◽  
Tadashi Kondo ◽  
Emyo Nakano ◽  
...  
Keyword(s):  
Vital Staining ◽  
Neutral Red ◽  
New Method

scholarly journals A Vital Staining Practice That Discerns Ancestry within Groups of Settling Larvae of a Brooding Coral

2021 ◽  
Vol 9 (6) ◽  
pp. 616
Author(s):  
Dor Shefy ◽  
Nadav Shashar ◽  
Baruch Rinkevich
Keyword(s):  
Settlement Patterns ◽  
Coral Species ◽  
Larval Settlement ◽  
Vital Staining ◽  
Neutral Red ◽  
Larval Metamorphosis ◽  
Coral Larvae ◽  
Stylophora Pistillata ◽  
Settlement Location ◽  
Experimental Tool

Xenogeneic and allogeneic encounters following aggregated and clustered settlements of coral larvae (planulae) may carry important ecological consequences in shaping coral reefs’ communities. However, larval settlement behaviors and settlement location choices in the presence of conspecifics or heterospecifics have not been examined in detail, due to a lack of experimental tools. One potential approach is the employment of vital staining of planulae with dyes that do not impair larval metamorphosis processes, are stable for prolonged periods, and do not diffuse to un-labeled counterpart planulae. For these purposes, we examined the use of neutral red (NR) dye, as an identification marker, on the planulae of Stylophora pistillata, a Red Sea branching coral species. To examine possible NR impacts on larval settlement in the presence of conspecific planulae, we followed the settlement ratios of kin, non-kin, and mixed assemblages, as a proxy for metamorphosis success. We found no differences in settlement rates of stained vs. unstained larvae, lack of stain diffusion to other larvae and that NR stain is maintained for more than a week under a still water regimen. Thus, staining with NR may serve as a useful experimental tool, opening new opportunities in studying larval settlement patterns in sessile marine organisms.

scholarly journals Vacuolar pH in yeast cells during pseudohyphal growth induced by nitrogen starvation

2017 ◽  
Author(s):  
Koji Makanae
Keyword(s):  
Intracellular Ph ◽  
Nitrogen Starvation ◽  
Vital Staining ◽  
Time Lapse ◽  
Neutral Red ◽  
Yeast Cells ◽  
Ph Indicator ◽  
Ph Gradients ◽  
Yeast Form ◽  
Pseudohyphal Growth

It has been reported that the intracellular pH of the budding yeast Saccharomyces cerevisiae is asymmetric between mother and daughter cells, and this asymmetry in pH underlies replicative aging and rejuvenation. S. cerevisiae growth morphology changes between the yeast form and pseudohyphal form, according to nutrient availability. A previous study reported that the replicative life span of pseudohyphal form cells is longer than that of yeast form cells in S. cerevisiae. However, the intracellular pH of pseudohyphal cells is unknown. To examine the intracellular pH of S. cerevisiae cells during pseudohyphal growth, vital staining was performed with neutral red, which is a pH indicator, of cells growing on nitrogen starvation (SLAD) medium. The results showed that the vacuoles of S. cerevisiae cells during pseudohyphal growth induced by nitrogen starvation formed polar pH gradients. The relationship between cell size and shape and the neutral red staining patterns suggested that the pH of cell vacuoles during pseudohyphal growth changed from uniformly near pH 6.8 to steep gradients of pH from vacuole ends along the long axis of the cell. The results of time-lapse imaging to examine vacuolar dynamics and neutral red staining suggested that the pH gradients were not formed simply by inheritance of vacuolar contents accompanying vacuolar movements.

The effect of physical agents on hydatid scolex viability

Parasitology ◽  
1949 ◽  
Vol 39 (3-4) ◽  
pp. 157-163 ◽  
Author(s):  
L. B. Fastier
Keyword(s):  
Cold Water ◽  
Culture Media ◽  
Wave Length ◽  
Ultra Violet ◽  
Vital Staining ◽  
Neutral Red ◽  
Simple Method ◽  
Hydatid Fluid ◽  
Infected Liver

By employing a combination of sub-stage heating and supra-vital staining with eosin or neutral red it was found that viable and non-viable hydatid scolices could be distinguished microscopically. Using this technique a study of the effect of heat in vitro on hydatid scolices has shown that death resulted from exposures at 50° C. for 70 min. and at 55° C. for 30 min.Experiments on the effect of boiling in destroying the contents of fertile cysts embedded in sheep liver showed that when offal was added directly to boiling water destruction of scolices was complete after 40 min. boiling. When the infected liver was placed in cold water brought to the boil, scolex death was assured only after 30 min. subsequent boiling.Ultra-violet radiations of 2537 A. did not kill scolices under the conditions adopted. Although this wave-length would apparently be ineffective as a scolicicidal agent in surgery of the human hydatid cyst, irradiation of hydatid fluid containing scolices might offer a simple method for initial sterilization of scolex culture media.The author wishes to acknowledge many helpful suggestions and loan of the Hanovia lamp by Dr G. M. Richardson of the Virus and Immunology Research Department, Otago Medical School.

Vital Staining with Neutral Red and Trypan Blue of3H-Thymidine-Labeled Cells Prior to Autoradiography

1967 ◽  
Vol 42 (3) ◽  
pp. 143-146 ◽  
Author(s):  
Wojciech Sawicki ◽  
Jørgen Kieler ◽  
Per Briand
Keyword(s):  
Trypan Blue ◽  
Vital Staining ◽  
Neutral Red
Sign in / Sign up

Export Citation Format

Share Document