Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms

Fusarium wilt is a devastating soil-borne disease caused mainly by highly host-specific formae speciales of Fusarium oxysporum. Antagonistic microorganisms play a very important role in Fusarium wilt control, and the isolation of potential biocontrol strains is becoming more and more important. We isolated a bacterial strain (SEM-9) from the high-temperature stage of silkworm excrement composting, which had a marked ability to solubilize phosphorus, promote the growth and increase the yield of the small Chinese cabbage, and which also exhibited considerable antagonistic effect towards Fusarium sambucinum and other fungi. The result of physiological and biochemical analyses, as well as genome sequencing, showed that SEM-9 was a strain of Bacillus subtilis. Through genome annotation and analysis, it was found that SEM-9 contained genes related to the regulation of biofilm formation, which may play an important role in colonization, and gene clusters encoding the biosynthesis of antimicrobials, such as surfactin, bacilysin, fengycin, and subtilosin-A. The production of such antifungal compounds may constitute the basis of the mode-of-action of SEM-9 against Fusarium spp. These data suggested that the SEM-9 strain has potential as both a biofertilizer and a biocontrol agent, with the potential to manage Fusarium wilt disease in crops.

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Plant Growth-Promoting Rhizobacteria StrainBacillus amyloliquefaciensNJN-6-Enriched Bio-organic Fertilizer Suppressed Fusarium Wilt and Promoted the Growth of Banana Plants

Journal of Agricultural and Food Chemistry ◽

10.1021/jf400038z ◽

2013 ◽

Vol 61 (16) ◽

pp. 3774-3780 ◽

Cited By ~ 66

Author(s):

Jun Yuan ◽

Yunze Ruan ◽

Beibei Wang ◽

Jian Zhang ◽

Raza Waseem ◽

...

Keyword(s):

Plant Growth ◽

Fusarium Wilt ◽

Organic Fertilizer ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Growth Promoting ◽

Growth Promoting

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Application of bio-organic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil

Biology and Fertility of Soils ◽

10.1007/s00374-012-0675-4 ◽

2012 ◽

Vol 48 (7) ◽

pp. 807-816 ◽

Cited By ~ 161

Author(s):

Meihua Qiu ◽

Ruifu Zhang ◽

Chao Xue ◽

Shusheng Zhang ◽

Shuqing Li ◽

...

Keyword(s):

Microbial Community ◽

Fusarium Wilt ◽

Rhizosphere Soil ◽

Organic Fertilizer

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Allelopathic Control Effect of Complex Formulation of Compound Microbial Community and Bio-Organic Fertilizer on Cucumber fusarium Wilt

Revista de Chimie ◽

10.37358/rc.20.4.8104 ◽

2020 ◽

Vol 71 (4) ◽

pp. 646-659

Author(s):

Lijin Qin ◽

Zengming Zhong ◽

Dandan Wang ◽

Haiqi Hu ◽

Duo Li ◽

...

Keyword(s):

Microbial Community ◽

Cucumis Sativus ◽

Fusarium Wilt ◽

Organic Fertilizer ◽

Disease Index ◽

Allelopathic Effect ◽

Soil Leaching ◽

Control Effect ◽

Complex Formulation ◽

Cucumber Fusarium Wilt

To study the influence of complex formulation of �WoFengKang� compound microbial community and bio-organic fertilizer on allelopathic effect of cucumber fusarium wilt and field growth traits, in this experiment, different indoor treatments were taken to cultivate cucumber fusarium wilt using soil leaching liquor and Cucumis sativus growth index, fusarium wilt disease index were determined, so that allelopathic control effect of different dosages of compound microbial community on cucumber fusarium wilt can be investigated. The results showed that: compared with conventional CK, cucumber fusarium wilt cultured in different soil leaching liquor had smaller diameter. Where, colony diameter in treatment 3 was the smallest, which was 36.72 mm. Compound microbial community had certain allelopathic effect on cucumber fusarium wilt, and inhibition effect was shown with allelopathic effect reaching 27.13~43.91%. The above differently-treated soil was loaded to seedling-raising pot for Cucumis sativus planting. Cucumber fusarium wilt (FOC) was inoculated in the rough leaf stage of Cucumis sativus, thus reducing the disease index of cucumber fusarium wilt with obvious control effect reaching 55.94~72.63%. Where, treatment 3 demonstrated the best effect and allelopathic control effect reached 72.63% after 15d inoculation. The complex formulation of the two promoted vegetative growth of Cucumis sativus, lowered node of the first female flower bud, increased the number of female flowers within 30 nodes, so that 3.25d early flowering and 4.75d delayed seedling raising were achieved. Therefore, application of microbial community amid and after Cucumis sativus field planting has certain allelopathic control effect on cucumber fusarium wilt, which can effectively reduce the incidence of cucumber fusarium wilt and provide a scientific basis for the allelopathic prevention and control of soil-borne diseases in Cucumis sativus.

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Fumigation coupled with bio-organic fertilizer for the suppression of watermelon Fusarium wilt disease re-shapes the soil microbiome

Applied Soil Ecology ◽

10.1016/j.apsoil.2019.04.007 ◽

2019 ◽

Vol 140 ◽

pp. 49-56 ◽

Cited By ~ 4

Author(s):

Chao Xue ◽

Zongzhuan Shen ◽

Yuewen Hao ◽

Sitian Yu ◽

Yuchan Li ◽

...

Keyword(s):

Fusarium Wilt ◽

Organic Fertilizer ◽

Wilt Disease ◽

Soil Microbiome ◽

Fusarium Wilt Disease

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Isolation of Bacillus subtilis strain SEM-2 from silkworm excrement and characterisation of its antagonistic effect against Fusarium spp.

Canadian Journal of Microbiology ◽

10.1139/cjm-2019-0621 ◽

2020 ◽

Vol 66 (6) ◽

pp. 401-412

Author(s):

Qingrong Li ◽

Sentai Liao ◽

Junhong Wei ◽

Dongxu Xing ◽

Yang Xiao ◽

...

Keyword(s):

Bacillus Subtilis ◽

Fusarium Wilt ◽

Fluorescent Protein ◽

Early Stage ◽

Gene Clusters ◽

Inhibitory Effect ◽

Antagonistic Effect ◽

Subtilis Strain ◽

Fusarium Spp ◽

Antagonistic Microorganisms

Fusarium wilt is a devastating soil-borne disease mainly caused by highly host-specific formae speciales of Fusarium spp. Antagonistic microorganisms play a very important role in Fusarium wilt control. Isolation of potential biocontrol strains has become increasingly important. Bacterial strain SEM-2 was isolated from the high-temperature stage of silkworm excrement composting. SEM-2 exhibited a considerable antagonistic effect against Fusarium graminearum mycelial growth and spore germination. The results of pot experiments suggested that SEM-2 has a better inhibitory effect on the early stage of disease occurrence. The green fluorescent protein labelled SEM-2 coated on the surface of tomato seeds colonised the roots of tomato plants in 15 days. Genome sequencing identified SEM-2 as a new strain of Bacillus subtilis, and genome annotation and analysis determined gene clusters related to the biosynthesis of antimicrobials, such as bacillaene, fengycin, bacillibactin, subtilosin A, surfactin, and bacilysin. Interestingly, liquid chromatography – quadrupole time-of-flight mass spectrometry revealed that metabolites in pathways associated with the synthesis of secondary metabolites and antibiotics were highly differentially expressed. These findings may help to explain the mode of action of B. subtilis SEM-2 against Fusarium spp.

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