Characterization and sequence analysis of potential biofertilizer and biocontrol agent Bacillus subtilis strain SEM-9 from silkworm excrement

2019 ◽  
Vol 65 (1) ◽  
pp. 45-58 ◽  
Author(s):  
Qingrong Li ◽  
Sentai Liao ◽  
Huyu Zhi ◽  
Dongxu Xing ◽  
Yang Xiao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Gene Clusters ◽  
Antagonistic Effect ◽  
Subtilis Strain ◽  
Fusarium Spp ◽  
Antagonistic Microorganisms ◽  
Biochemical Analyses ◽  
Physiological And Biochemical

Fusarium wilt is a devastating soil-borne disease caused mainly by highly host-specific formae speciales of Fusarium oxysporum. Antagonistic microorganisms play a very important role in Fusarium wilt control, and the isolation of potential biocontrol strains is becoming more and more important. We isolated a bacterial strain (SEM-9) from the high-temperature stage of silkworm excrement composting, which had a marked ability to solubilize phosphorus, promote the growth and increase the yield of the small Chinese cabbage, and which also exhibited considerable antagonistic effect towards Fusarium sambucinum and other fungi. The result of physiological and biochemical analyses, as well as genome sequencing, showed that SEM-9 was a strain of Bacillus subtilis. Through genome annotation and analysis, it was found that SEM-9 contained genes related to the regulation of biofilm formation, which may play an important role in colonization, and gene clusters encoding the biosynthesis of antimicrobials, such as surfactin, bacilysin, fengycin, and subtilosin-A. The production of such antifungal compounds may constitute the basis of the mode-of-action of SEM-9 against Fusarium spp. These data suggested that the SEM-9 strain has potential as both a biofertilizer and a biocontrol agent, with the potential to manage Fusarium wilt disease in crops.

Isolation of Bacillus subtilis strain SEM-2 from silkworm excrement and characterisation of its antagonistic effect against Fusarium spp.

2020 ◽  
Vol 66 (6) ◽  
pp. 401-412
Author(s):  
Qingrong Li ◽  
Sentai Liao ◽  
Junhong Wei ◽  
Dongxu Xing ◽  
Yang Xiao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Wilt ◽  
Fluorescent Protein ◽  
Early Stage ◽  
Gene Clusters ◽  
Inhibitory Effect ◽  
Antagonistic Effect ◽  
Subtilis Strain ◽  
Fusarium Spp ◽  
Antagonistic Microorganisms

Fusarium wilt is a devastating soil-borne disease mainly caused by highly host-specific formae speciales of Fusarium spp. Antagonistic microorganisms play a very important role in Fusarium wilt control. Isolation of potential biocontrol strains has become increasingly important. Bacterial strain SEM-2 was isolated from the high-temperature stage of silkworm excrement composting. SEM-2 exhibited a considerable antagonistic effect against Fusarium graminearum mycelial growth and spore germination. The results of pot experiments suggested that SEM-2 has a better inhibitory effect on the early stage of disease occurrence. The green fluorescent protein labelled SEM-2 coated on the surface of tomato seeds colonised the roots of tomato plants in 15 days. Genome sequencing identified SEM-2 as a new strain of Bacillus subtilis, and genome annotation and analysis determined gene clusters related to the biosynthesis of antimicrobials, such as bacillaene, fengycin, bacillibactin, subtilosin A, surfactin, and bacilysin. Interestingly, liquid chromatography – quadrupole time-of-flight mass spectrometry revealed that metabolites in pathways associated with the synthesis of secondary metabolites and antibiotics were highly differentially expressed. These findings may help to explain the mode of action of B. subtilis SEM-2 against Fusarium spp.

scholarly journals Newly Isolated Streptomyces sp. JBS5-6 as a Potential Biocontrol Agent to Control Banana Fusarium Wilt: Genome Sequencing and Secondary Metabolite Cluster Profiles

2020 ◽  
Vol 11 ◽  
Author(s):  
Tao Jing ◽  
Dengbo Zhou ◽  
Miaoyi Zhang ◽  
Tianyan Yun ◽  
Dengfeng Qi ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Complete Genome ◽  
Biocontrol Agent ◽  
Fermentation Broth ◽  
Membrane Integrity ◽  
Gene Clusters ◽  
Wilt Disease ◽  
Gas Chromatography Mass Spectrometry ◽  
Fusarium Wilt Disease ◽  
Potential Biocontrol Agent

Banana is a key staple food and fruit in countries all over the world. However, the development of the global banana industry is seriously threatened by Fusarium wilt disease, which is caused by Fusarium oxysporum f. sp. cubense (Foc). In particular, Foc tropical race 4 (Foc TR4) could infect more than 80% of global banana and plantain crops. Until now, there were no commercial chemicals or resistant cultivars available to control the disease. Biological control using actinomycetes is considered a promising strategy. In this study, 88 actinomycetes were isolated from a banana orchard without symptoms of Fusarium wilt disease for more than 10 years. An actinobacterial strain labeled as JBS5-6 has exhibited strong antifungal activities against Foc TR4 and other selected 10 phytopathogenic fungi. Based on phenotypic and biochemical traits as well as complete genome analysis, strain JBS5-6 was assigned to Streptomyces violaceusniger. Extracts of the strain inhibited the mycelial growth and spore germination of Foc TR4 by destroying membrane integrity and the ultrastructure of cells. The complete genome of strain JBS5-6 was sequenced and revealed a number of key function gene clusters that contribute to the biosynthesis of active secondary metabolites. Sixteen chemical compounds were further identified by gas chromatography-mass spectrometry (GC-MS). 5-hydroxymethyl-2-furancarboxaldehyde was one of the dominant components in strain JBS5-6 extracts. Moreover, fermentation broth of strain JBS5-6 significantly reduced the disease index of banana seedlings by inhibiting the infection of Foc TR4 in a pot experiment. Hence, strain JBS5-6 is a potential biocontrol agent for the management of disease and the exploitation of biofertilizer.

Characterization of Mesorhizobium strains for salt tolerance and wilt control: Their potential for plant growth promotion of chickpea (Cicer arietinum L.)

2018 ◽  
Author(s):  
Anju Sehrawat ◽  
Aakanksha Khandelwal ◽  
Satyavir Singh Sindhu
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Maximum Increase ◽  
Shoot Dry Weight ◽  
Plant Growth Promoting

Mesorhizobium sp. indirectly promote the growth of plants as a biocontrol agent by inhibiting the growth of pathogens particularly Fusarium wilt of chickpea. Out of 24 Mesorhizobium isolates obtained from chickpea nodules, eight isolates showed antagonistic effect against Fusarium oxysporum. Salinity stress severely affects growth, nodulation and yield of chickpea. Mesorhizobium isolates were tested for their salt tolerance capacity at 1, 2, 4, 6 and 8% NaCl concentrations. Only two Mesorhizobium isolates MCA5 and MCA22 were found salt-tolerant upto 8% of salt concentration. Maximum increase (45.5%) in shoot dry weight was observed by inoculation of isolate MCA20 at 40 days of chickpea growth under chillum jar conditions, whereas isolate MCA23 resulted in 166.2% increase in root dry weight. Likewise, 112.6% increase in shoot dry weight was observed on inoculation of MCA14 isolate at 80th day of observation. Further extensive research is required to understand the mechanism of potential Mesorhizobium isolates of chickpea in controlling Fusarium wilt disease and salt tolerance. Selection of mesorhizobia with twin functional traits (plant growth promoting and biocontrol agent) can be exploited as future biofertilizer in chickpea.

scholarly journals Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber

2019 ◽  
Vol 20 (20) ◽  
pp. 5240 ◽  
Author(s):  
Yunlong Li ◽  
Sanfeng Chen
Keyword(s):  
Fusarium Wilt ◽  
Mutation Analysis ◽  
Biocontrol Agent ◽  
Paenibacillus Polymyxa ◽  
Gene Clusters ◽  
Wilt Disease ◽  
Systemic Resistance ◽  
Antibiotic Biosynthesis ◽  
Fusarium Wilt Disease ◽  
First Time

Cucumber is an important vegetable crop in China. Fusarium wilt is a soil-borne disease that can significantly reduce cucumber yields. Paenibacillus polymyxa WLY78 can strongly inhibit Fusarium oxysporum f. sp. Cucumerium, which causes Fusarium wilt disease. In this study, we screened the genome of WLY78 and found eight potential antibiotic biosynthesis gene clusters. Mutation analysis showed that among the eight clusters, the fusaricidin synthesis (fus) gene cluster is involved in inhibiting the Fusarium genus, Verticillium albo-atrum, Monilia persoon, Alternaria mali, Botrytis cinereal, and Aspergillus niger. Further mutation analysis revealed that with the exception of fusTE, the seven genes fusG, fusF, fusE, fusD, fusC, fusB, and fusA within the fus cluster were all involved in inhibiting fungi. This is the first time that demonstrated that fusTE was not essential. We first report the inhibitory mode of fusaricidin to inhibit spore germination and disrupt hyphal membranes. A biocontrol assay demonstrated that fusaricidin played a major role in controlling Fusarium wilt disease. Additionally, qRT-PCR demonstrated that fusaricidin could induce systemic resistance via salicylic acid (SA) signal against Fusarium wilt of cucumber. WLY78 is the first reported strain to both produce fusaricidin and fix nitrogen. Therefore, our results demonstrate that WLY78 will have great potential as a biocontrol agent in agriculture.

scholarly journals Formulation development of the biocontrol agent Bacillus subtilis strain CPA-8 by spray-drying

2012 ◽  
Vol 112 (5) ◽  
pp. 954-965 ◽  
Author(s):  
V. Yánez-Mendizábal ◽  
I. Viñas ◽  
J. Usall ◽  
R. Torres ◽  
C. Solsona ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Spray Drying ◽  
Biocontrol Agent ◽  
Subtilis Strain ◽  
Formulation Development

scholarly journals In Vitro Evaluation and Genome Mining of Bacillus subtilis Strain RS10 Reveals Its Biocontrol and Plant Growth-Promoting Potential

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1273
Author(s):  
Sajid Iqbal ◽  
Nimat Ullah ◽  
Hussnain Ahmed Janjua
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Genome Analysis ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Subtilis Strain ◽  
Antifungal Metabolites ◽  
Plant Growth Promoting ◽  
Growth Promoting

Recently, crop management has involved excessive use of chemical fertilizers and pesticides, compromising public health and environmental integrity. Rhizobacteria, which can enhance plant growth and protect plants from phytopathogen, are eco-friendly and have been attracting increasing attention. In the current study, Bacillus subtilis RS10 isolated from the rhizosphere region of Cynodon dactylon, inhibited the growth of indicator strains and exhibited in vitro plant growth-promoting traits. A whole-genome analysis identified numerous biosynthetic gene clusters encoding antibacterial and antifungal metabolites including bacillibactin, bogorol A, fengycin, bacteriocin, type III polyketides (PKs), and bacilysin. The plant growth-promoting conferring genes involved in nitrogen metabolism, phosphate solubilization, hydrogen sulfide, phytohormones, siderophore biosynthesis, chemotaxis and motility, plant root colonization, lytic enzymes, and biofilm formation were determined. Furthermore, genes associated with abiotic stresses such as high salinity and osmotic stress were identified. A comparative genome analysis indicated open pan-genome and the strain was identified as a novel sequence type (ST-176). In addition, several horizontal gene transfer events were found which putatively play a vital role in the evolution and new functionalities of a strain. In conclusion, the current study demonstrates the potential of RS10 antagonism against important pathogens and plant growth promotion, highlighting its application in sustainable agriculture.

scholarly journals The activity of native Bacillus subtilis strains in control of green mould disease of oyster mushroom (Pleurotus spp.)

2019 ◽  
Vol 34 (2) ◽  
pp. 97-102
Author(s):  
Ivana Potocnik ◽  
Svetlana Milijasevic-Marcic ◽  
Olja Stanojevic ◽  
Tanja Beric ◽  
Slavisa Stankovic ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Inhibition ◽  
Antagonistic Activity ◽  
Oyster Mushroom ◽  
Subtilis Strain ◽  
Mushroom Production ◽  
Antagonistic Microorganisms ◽  
Green Mould ◽  
Antagonistic Potential

The study aimed to isolate potential biocontrol agents from mushroom substrate that could serve as an alternative to toxic chemicals commonly used for disease control in mushroom production. The antagonistic potential of ten native Bacillus subtilis strains against the causal agents of green mould disease of oyster mushroom, Trichoderma pleuroti and Trichoderma pleuroticola, was evaluated. The antagonistic potential of Bacillus spp. strains was quantified in vitro based on dual cultivation with the pathogen. Growth inhibition of T. pleuroti ranged from 54.44% to 62.22% and no significant differences in antagonistic activity were found between the tested B. subtilis strains. Inhibition of T. pleuroticola was slightly higher, ranging from 55.56% to 69.62% and B. subtilis strain B-358 induced the highest growth inhibition. This research confirmed mushroom substrate to be a good source of antagonistic microorganisms with potentials for use in biological control of green mould in oyster mushroom production.

scholarly journals Draft Genome Sequences of a Bacillus subtilis Strain, a Bacillus velezensis Strain, a Paenibacillus Strain, and an Acinetobacter baumannii Strain, All Isolated from the Phyllosphere of Lactuca sativa or Solanum lycopersicum

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Claudia Y. Muñoz ◽  
Anne de Jong ◽  
Oscar P. Kuipers
Keyword(s):  
Bacillus Subtilis ◽  
Acinetobacter Baumannii ◽  
Solanum Lycopersicum ◽  
Lactuca Sativa ◽  
Draft Genome ◽  
Gene Clusters ◽  
Subtilis Strain ◽  
Bacillus Velezensis ◽  
Genome Sequences ◽  
Microbe Interactions

ABSTRACT Four strains isolated from tomato and lettuce phyllosphere were sequenced in order to investigate the presence of novel antimicrobial gene clusters and to get a better understanding of plant microbe interactions. These strains comprise two Bacillus strains, one Paenibacillus strain, and one Acinetobacter strain.

scholarly journals A novel antifungal peptide purified from Bacillus subtilis strain EDR4

2016 ◽  
Vol 68 (2) ◽  
pp. 319-324
Author(s):  
Jingyuan Ji ◽  
Jie Yang ◽  
Lili Huang ◽  
Zhensheng Kang
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Biocontrol Agent ◽  
Pathogenic Fungi ◽  
Subtilis Strain ◽  
Antifungal Peptide ◽  
Inhibitory Effects ◽  
Canker Disease ◽  
Ph Values ◽  
Potential Biocontrol Agent

Bacillus subtilis strain EDR4 is a potential biocontrol agent against Valsa mali (Vm), the fungus causing apple canker disease. In this study, we identified and characterized the antifungal peptide produced by B. subtilis EDR4. Peptides were isolated by 30% ammonium sulfate precipitation and purified by column chromatography. A 4-kDa peptide exhibiting antifungal activity was obtained and designated as P6. The amino acid sequence of P6 was determined by liquid chromatography combined with tandem mass spectrometry. P6 showed inhibitory effects against eight different pathogenic plant fungi, and was stable (i.e., retained its biological activity) at temperatures as high as 121?C for 20 min and at pH values ranging from 3 to 11. Microscopic examination of Vm hyphae treated with P6 showed protoplasm release, and in vitrospore germination was also inhibited. These results suggest that P6 is the active substance responsible for the antifungal activity of B. subtilisEDR4 against Vmand that P6 may be effective in the biocontrol of Vmas well as other plant pathogenic fungi.

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