Short peptide tags increase the yield of C-terminally labeled protein

2007 ◽  
Vol 29 (7) ◽  
pp. 1065-1073 ◽  
Author(s):  
Teruaki Kobayashi ◽  
Miwa Shiratori ◽  
Hirofumi Nakano ◽  
Chikashi Eguchi ◽  
Makoto Shirai ◽  
...  
Keyword(s):  
Short Peptide ◽  
Peptide Tags

Analysis and Control of Protein Crystallization Using Short Peptide Tags That Change Solubility without Affecting Structure, Thermal Stability, and Function

2015 ◽  
Vol 15 (6) ◽  
pp. 2703-2711 ◽  
Author(s):  
Mohammad Monirul Islam ◽  
Shigeyoshi Nakamura ◽  
Keiichi Noguchi ◽  
Masafumi Yohda ◽  
Shun-ichi Kidokoro ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Protein Crystallization ◽  
Short Peptide ◽  
Peptide Tags ◽  
And Function ◽  
And Control

Genetically Encoded Short Peptide Tags for Orthogonal Protein Labeling by Sfp and AcpS Phosphopantetheinyl Transferases

2007 ◽  
Vol 2 (5) ◽  
pp. 337-346 ◽  
Author(s):  
Zhe Zhou ◽  
Pablo Cironi ◽  
Alison J. Lin ◽  
Yangqing Xu ◽  
Siniša Hrvatin ◽  
...  
Keyword(s):  
Protein Labeling ◽  
Short Peptide ◽  
Peptide Tags ◽  
Phosphopantetheinyl Transferases

Detecting ligand-protein interactions inside cells using reactive peptide tags and split luciferase

2021 ◽  
Author(s):  
Tsuyoshi Takahashi ◽  
Hiroaki Hagiwara
Keyword(s):  
Protein Interactions ◽  
Short Peptide ◽  
Nanoluc Luciferase ◽  
Peptide Tags ◽  
Split Luciferase

We report a method for detecting ligand-protein interactions occurring within cells using short peptide reactive tags appended to ligands and proteins, along with a split NanoLuc luciferase. This method can...

Mutational analysis of protein solubility enhancement using short peptide tags

Biopolymers ◽  
2007 ◽  
Vol 85 (1) ◽  
pp. 12-18 ◽  
Author(s):  
Atsushi Kato ◽  
Kosuke Maki ◽  
Teppei Ebina ◽  
Kunihiro Kuwajima ◽  
Kunitsugu Soda ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Protein Solubility ◽  
Solubility Enhancement ◽  
Short Peptide ◽  
Peptide Tags

scholarly journals Improving the robustness of engineered bacteria to nutrient stress using programmed proteolysis

2021 ◽  
Author(s):  
Klara Szydlo ◽  
Zoya Ignatova ◽  
Thomas E Gorochowski
Keyword(s):  
Messenger Rna ◽  
Essential Amino Acids ◽  
Secondary Source ◽  
Genetic Circuits ◽  
Reporter Protein ◽  
Short Peptide ◽  
E Coli ◽  
Peptide Tags ◽  
Gene Expression Dynamics ◽  
Engineered Bacteria

The use of short peptide tags in synthetic genetic circuits allows for the tuning of gene expression dynamics and the freeing of amino acid resources through targeted protein degradation. Here, we use elements of the Escherichia coli and Mesoplasma florum transfer-messenger RNA (tmRNA) ribosome rescue systems to compare endogenous and foreign proteolysis systems in E. coli. We characterize the performance and burden of each and show that while both greatly shorten the half-life of a tagged protein, the endogenous system is approximately seven times more efficient. Based on these results, we then show how proteolysis can be used to improve cellular robustness through targeted degradation of a reporter protein in auxotrophic strains, providing a limited secondary source of essential amino acids that help partially restore growth when nutrients become scarce. These findings provide avenues for controlling the functional lifetime of engineered cells once deployed and increasing their tolerance to fluctuations in nutrient availability.

A Novel Method to Analyze Nucleocytoplasmic Transport In Vivo by Using Short Peptide Tags

2013 ◽  
Vol 425 (10) ◽  
pp. 1839-1845 ◽  
Author(s):  
Cornelius Wimmer ◽  
Stephanie Platzer ◽  
Wolfgang Hillen ◽  
Marcus Klotzsche
Keyword(s):  
Nucleocytoplasmic Transport ◽  
Short Peptide ◽  
Peptide Tags ◽  
Novel Method
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