Molecular cloning and functional expression of two key carotene synthetic genes derived from Blakeslea trispora into E. coli for increased β-carotene production

2012 ◽  
Vol 34 (11) ◽  
pp. 2077-2082 ◽  
Author(s):  
Jie Sun ◽  
Xin-Xiao Sun ◽  
Ping-Wah Tang ◽  
Qi-Peng Yuan
Keyword(s):  
Molecular Cloning ◽  
Functional Expression ◽  
Blakeslea Trispora ◽  
Synthetic Genes ◽  
E Coli ◽  
Β Carotene

scholarly journals Molecular cloning and functional expression in E. coli of a novel plant enzyme mediating ξ-carotene desaturation

FEBS Letters ◽  
1995 ◽  
Vol 372 (2-3) ◽  
pp. 199-202 ◽  
Author(s):  
Manuela Albrecht ◽  
Annelyse Klein ◽  
Philippe Hugueney ◽  
Gerhard Sandmann ◽  
Marcel Kuntz
Keyword(s):  
Molecular Cloning ◽  
Functional Expression ◽  
E Coli ◽  
Plant Enzyme

scholarly journals Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masuzu Kikuchi ◽  
Keiichi Kojima ◽  
Shin Nakao ◽  
Susumu Yoshizawa ◽  
Shiho Kawanishi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Proton Pump ◽  
Expression System ◽  
Spectroscopic Characterization ◽  
Functional Expression ◽  
Proton Pumping ◽  
E Coli ◽  
Oxyrrhis Marina ◽  
Domains Of Life

AbstractMicrobial rhodopsins are photoswitchable seven-transmembrane proteins that are widely distributed in three domains of life, archaea, bacteria and eukarya. Rhodopsins allow the transport of protons outwardly across the membrane and are indispensable for light-energy conversion in microorganisms. Archaeal and bacterial proton pump rhodopsins have been characterized using an Escherichia coli expression system because that enables the rapid production of large amounts of recombinant proteins, whereas no success has been reported for eukaryotic rhodopsins. Here, we report a phylogenetically distinct eukaryotic rhodopsin from the dinoflagellate Oxyrrhis marina (O. marina rhodopsin-2, OmR2) that can be expressed in E. coli cells. E. coli cells harboring the OmR2 gene showed an outward proton-pumping activity, indicating its functional expression. Spectroscopic characterization of the purified OmR2 protein revealed several features as follows: (1) an absorption maximum at 533 nm with all-trans retinal chromophore, (2) the possession of the deprotonated counterion (pKa = 3.0) of the protonated Schiff base and (3) a rapid photocycle through several distinct photointermediates. Those features are similar to those of known eukaryotic proton pump rhodopsins. Our successful characterization of OmR2 expressed in E. coli cells could build a basis for understanding and utilizing eukaryotic rhodopsins.

scholarly journals Purification, Molecular Cloning, and Functional Expression of the Human Nicodinamide-Adenine Dinucleotide Phosphate-Regulated Thyroid Hormone-Binding Protein

1997 ◽  
Vol 11 (11) ◽  
pp. 1728-1736 ◽  
Author(s):  
Marie-Pierre Vié ◽  
Claudine Evrard ◽  
Jeannine Osty ◽  
Aline Breton-Gilet ◽  
Pascal Blanchet ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Molecular Cloning ◽  
Binding Protein ◽  
Functional Expression ◽  
Hormone Binding ◽  
Hormone Binding Protein

scholarly journals Molecular cloning, sequencing, and functional expression of a cDNA encoding human coproporphyrinogen oxidase.

1994 ◽  
Vol 91 (8) ◽  
pp. 3024-3028 ◽  
Author(s):  
P. Martasek ◽  
J. M. Camadro ◽  
M. H. Delfau-Larue ◽  
J. B. Dumas ◽  
J. J. Montagne ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Expression ◽  
Coproporphyrinogen Oxidase

scholarly journals Molecular Cloning and Functional Expression of zfCx52.6

2003 ◽  
Vol 279 (4) ◽  
pp. 2913-2921 ◽  
Author(s):  
Georg Zoidl ◽  
Roberto Bruzzone ◽  
Svenja Weickert ◽  
Marian Kremer ◽  
Christiane Zoidl ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Expression

Molecular Cloning of a cDNA Encoding Ribosome Inactivating Protein from Amaranthus viridis and Its Expression in E. coli

2000 ◽  
Vol 10 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Suk-Yoon Kwon ◽  
Chung Sun An ◽  
Jang Ryol Liu ◽  
Sang-Soo Kwak ◽  
Haeng-Soon Lee ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Ribosome Inactivating Protein ◽  
E Coli
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