Cloning and sequence analysis of the heat-stable acrylamidase from a newly isolated thermophilic bacterium, Geobacillus thermoglucosidasius AUT-01

2012 ◽  
Vol 24 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Minseok Cha ◽  
Glenn H. Chambliss
Keyword(s):  
Sequence Analysis ◽  
Thermophilic Bacterium ◽  
Geobacillus Thermoglucosidasius ◽  
Heat Stable

scholarly journals Draft Genome Sequence of a Thermophilic Desulfurization Bacterium,Geobacillus thermoglucosidasiusStrain W-2

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Lin Zhu ◽  
Mingchang Li ◽  
Shuyi Guo ◽  
Wei Wang
Keyword(s):  
Genome Sequence ◽  
Genetic Basis ◽  
Draft Genome ◽  
Northern China ◽  
Thermophilic Bacterium ◽  
Draft Genome Sequence ◽  
Oil Reservoir ◽  
Organosulfur Compounds ◽  
Deep Subsurface ◽  
Geobacillus Thermoglucosidasius

Geobacillus thermoglucosidasiusstrain W-2 is a thermophilic bacterium isolated from a deep-subsurface oil reservoir in northern China, which is capable of degrading organosulfur compounds. Here, we report the draft genome sequence ofG. thermoglucosidasiusstrain W-2, which may help to elucidate the genetic basis of biodegradation of organosulfur pollutants under heated conditions.

scholarly journals A Group II Intron-Type Open Reading Frame from the Thermophile Bacillus (Geobacillus) stearothermophilus Encodes a Heat-Stable Reverse Transcriptase

2004 ◽  
Vol 70 (12) ◽  
pp. 7140-7147 ◽  
Author(s):  
Jaishree Vellore ◽  
Samuel E. Moretz ◽  
Bert C. Lampson
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcription ◽  
Protein Product ◽  
Thermophilic Bacterium ◽  
Full Length ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Heat Stable ◽  
Heat Stable Protein ◽  
Group Ii

ABSTRACT The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the synthesis of full-length cDNAs is frequently inefficient, because the RT commonly used often produces truncated cDNAs. Synthesizing cDNA at higher temperatures, on the other hand, can provide a number of improvements. These include increasing the length of cDNA product, greater accuracy, and greater specificity during reverse transcription. Thus, an RT that remains stable and active at hot temperatures may produce better-quality cDNAs and improve the yield of full-length cDNAs. Described here is the discovery of a gene, designated trt, from the genome of the thermophilic bacterium Bacillus (Geobacillus) stearothermophilus strain 10. The gene codes for an open reading frame (ORF) similar to the ORFs encoded by group II introns found in bacteria. The gene was cloned and overexpressed in Escherichia coli, and its protein product was partially purified. Like the host organism, the Trt protein is a heat-stable protein with RT activity and can reverse transcribe RNA at temperatures as high as 75°C.

scholarly journals Heat-stable amylase complex produced by a strictly anaerobic and extremely thermophilic bacterium, Dictyoglomus thermophilum.

1988 ◽  
Vol 52 (2) ◽  
pp. 615-616 ◽  
Author(s):  
Yasuhiko KOBAYASHI ◽  
Mahoko MOTOIKE ◽  
Shoji FUKUZUMI ◽  
Toyoji OHSHIMA ◽  
Takashi SAIKI ◽  
...  
Keyword(s):  
Thermophilic Bacterium ◽  
Strictly Anaerobic ◽  
Heat Stable ◽  
Dictyoglomus Thermophilum

scholarly journals Heat-stable Amylase Complex Produced by a Strictly Anaerobic and Extremely Thermophilic Bacterium,Dictyoglomus thermophilum

1988 ◽  
Vol 52 (2) ◽  
pp. 615-616
Author(s):  
Yasuhiko Kobayashi ◽  
Mahoko Motoike ◽  
Shoji Fukuzumi ◽  
Toyoji Ohshima ◽  
Takashi Saiki ◽  
...  
Keyword(s):  
Thermophilic Bacterium ◽  
Strictly Anaerobic ◽  
Heat Stable ◽  
Dictyoglomus Thermophilum
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