Potential of a novel scaffold composed of human platelet lysate and fibrin for human corneal endothelial cells

2021 ◽  
Author(s):  
Mohammad Amir Mishan ◽  
Sahar Balagholi ◽  
Tahereh Chamani ◽  
Sepehr Feizi ◽  
Zahra-Soheila Soheili ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells ◽  
Human Platelet Lysate ◽  
Novel Scaffold

scholarly journals Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor

2021 ◽  
Author(s):  
Mohammad Amir Mishan ◽  
Sahar Balagholi ◽  
Tahereh Chamani ◽  
Sepehr Feizi ◽  
Zahra-Soheila Soheili ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Endothelial Cells ◽  
Rock Inhibitor ◽  
Treatment Groups ◽  
Medium Supplement ◽  
Proliferative Effect ◽  
Human Platelet Lysate

Purpose: Corneal endothelial cell (CEC) therapy can be used as a promising therapeutic option for patients with various corneal endothelial dysfunctions. In this study, we compared the proliferative effect of human platelet lysate (HPL), as a xeno-free medium supplement, with Y-27632 Rho/rho-associated protein kinase (ROCK) inhibitor, as a wellknown proliferative and adhesive agent for CECs, and fetal bovine serum (FBS) as the control, in the culture medium of human corneal endothelial cells (HCECs). Methods: We isolated HCECs from human donors and treated the cells as three different treatment groups including 20% HPL only, 10 μM Y-27632 ROCK inhibitor, combination of 20% HPL and 10 μM Y-27632 ROCK inhibitor, and 20% FBS as the control group. ELISA cell proliferation assay and cell counting was performed on the treated cells. Finally, HCECs were characterized by morphology and immunocytochemistry (ICC). Results: There was no significant proliferative effect of HPL on cell proliferation compared with the cells treated with Y-27632 ROCK inhibitor or the combination of HPL and Y-27632 ROCK inhibitor, but all the respected treatments had significant inducible effect on cell proliferation as compared with FBS-treated cells. The cells grown in all three treatment groups exhibited CEC morphology. Also, there was a higher expression of Na+/K+-ATPase and ZO-1, as CEC characteristic markers, in the culture of HCECs treated with HPL as compared with FBS. Conclusion: HPL offers a xeno−free and affordable medium supplement for CEC expansion that can be used in clinical applications.

Human platelet lysate is a feasible candidate to replace fetal calf serum as medium supplement for blood vascular and lymphatic endothelial cells

Cytotherapy ◽  
2014 ◽  
Vol 16 (9) ◽  
pp. 1238-1244 ◽  
Author(s):  
Pablo Hofbauer ◽  
Sabrina Riedl ◽  
Karin Witzeneder ◽  
Florian Hildner ◽  
Susanne Wolbank ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fetal Calf Serum ◽  
Human Platelet ◽  
Calf Serum ◽  
Platelet Lysate ◽  
Lymphatic Endothelial Cells ◽  
Medium Supplement ◽  
Human Platelet Lysate

Culturing Discarded Peripheral Human Corneal Endothelial Cells From the Tissues Deemed for Preloaded DMEK Transplants

Cornea ◽  
2019 ◽  
Vol 38 (9) ◽  
pp. 1175-1181 ◽  
Author(s):  
Mohit Parekh ◽  
Vito Romano ◽  
Alessandro Ruzza ◽  
Stephen B. Kaye ◽  
Diego Ponzin ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Human platelet lysate delivered via an ocular wound chamber for the treatment of corneal epithelial injuries

2021 ◽  
Vol 206 ◽  
pp. 108493
Author(s):  
Gina L. Griffith ◽  
Andrew W. Holt ◽  
Elof Eriksson ◽  
Anthony J. Johnson ◽  
Jennifer S. McDaniel
Keyword(s):  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Epithelial ◽  
Human Platelet Lysate

scholarly journals Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful Resource for Tissue Engineering

2017 ◽  
Vol 14 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Yongsong Liu ◽  
Hong Sun ◽  
Min Hu ◽  
Min Zhu ◽  
Sean Tighe ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Inactivated human platelet lysate with psoralen: a new perspective for mesenchymal stromal cell production in Good Manufacturing Practice conditions

Cytotherapy ◽  
2014 ◽  
Vol 16 (6) ◽  
pp. 750-763 ◽  
Author(s):  
Sara Castiglia ◽  
Katia Mareschi ◽  
Luciana Labanca ◽  
Graziella Lucania ◽  
Marco Leone ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Human Platelet ◽  
Good Manufacturing Practice ◽  
Platelet Lysate ◽  
Cell Production ◽  
Human Platelet Lysate ◽  
New Perspective
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